PMID- 26283971
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150818
LR  - 20201001
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 6
DP  - 2015
TI  - Hypoxanthine-guanine phosphoribosyltransferase and inosine 5'-monophosphate 
      dehydrogenase activities in three mammalian species: aquatic (Mirounga 
      angustirostris), semi-aquatic (Lontra longicaudis annectens) and terrestrial (Sus 
      scrofa).
PG  - 212
LID - 10.3389/fphys.2015.00212 [doi]
LID - 212
AB  - Aquatic and semiaquatic mammals have the capacity of breath hold (apnea) diving. 
      Northern elephant seals (Mirounga angustirostris) have the ability to perform 
      deep and long duration dives; during a routine dive, adults can hold their breath 
      for 25 min. Neotropical river otters (Lontra longicaudis annectens) can hold 
      their breath for about 30 s. Such periods of apnea may result in reduced oxygen 
      concentration (hypoxia) and reduced blood supply (ischemia) to tissues. 
      Production of adenosine 5'-triphosphate (ATP) requires oxygen, and most mammalian 
      species, like the domestic pig (Sus scrofa), are not adapted to tolerate hypoxia 
      and ischemia, conditions that result in ATP degradation. The objective of this 
      study was to explore the differences in purine synthesis and recycling in 
      erythrocytes and plasma of three mammalian species adapted to different 
      environments: aquatic (northern elephant seal) (n = 11), semiaquatic (neotropical 
      river otter) (n = 4), and terrestrial (domestic pig) (n = 11). Enzymatic activity 
      of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) was determined by 
      spectrophotometry, and activity of inosine 5'-monophosphate dehydrogenase (IMPDH) 
      and the concentration of hypoxanthine (HX), inosine 5'-monophosphate (IMP), 
      adenosine 5'-monophosphate (AMP), adenosine 5'-diphosphate (ADP), ATP, guanosine 
      5'-diphosphate (GDP), guanosine 5'-triphosphate (GTP), and xanthosine 
      5'-monophosphate (XMP) were determined by high-performance liquid chromatography 
      (HPLC). The activities of HGPRT and IMPDH and the concentration of HX, IMP, AMP, 
      ADP, ATP, GTP, and XMP in erythrocytes of domestic pigs were higher than in 
      erythrocytes of northern elephant seals and river otters. These results suggest 
      that under basal conditions (no diving, sleep apnea or exercise), aquatic, and 
      semiaquatic mammals have less purine mobilization than their terrestrial 
      counterparts.
FAU - Barjau Perez-Milicua, Myrna
AU  - Barjau Perez-Milicua M
AD  - Programa de Planeacion Ambiental y Conservacion, Laboratorio de Estres Oxidativo, 
      Centro de Investigaciones Biologicas del Noroeste La Paz, Mexico.
FAU - Zenteno-Savin, Tania
AU  - Zenteno-Savin T
AD  - Programa de Planeacion Ambiental y Conservacion, Laboratorio de Estres Oxidativo, 
      Centro de Investigaciones Biologicas del Noroeste La Paz, Mexico.
FAU - Crocker, Daniel E
AU  - Crocker DE
AD  - Department of Biology, Sonoma State University Rohnert Park, CA, USA.
FAU - Gallo-Reynoso, Juan P
AU  - Gallo-Reynoso JP
AD  - Laboratorio de Ecofisiologia, Centro de Investigacion en Alimentacion y 
      Desarrollo Guaymas, Mexico.
LA  - eng
PT  - Journal Article
DEP - 20150729
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC4518566
OTO - NOTNLM
OT  - hypoxanthine-guanine phosphorybosiltransferase
OT  - hypoxia
OT  - inosine 5'-monophosphate dehydrogenase
OT  - purine salvage
OT  - purine synthesis
EDAT- 2015/08/19 06:00
MHDA- 2015/08/19 06:01
PMCR- 2015/07/29
CRDT- 2015/08/19 06:00
PHST- 2015/03/06 00:00 [received]
PHST- 2015/07/14 00:00 [accepted]
PHST- 2015/08/19 06:00 [entrez]
PHST- 2015/08/19 06:00 [pubmed]
PHST- 2015/08/19 06:01 [medline]
PHST- 2015/07/29 00:00 [pmc-release]
AID - 10.3389/fphys.2015.00212 [doi]
PST - epublish
SO  - Front Physiol. 2015 Jul 29;6:212. doi: 10.3389/fphys.2015.00212. eCollection 
      2015.