PMID- 26286503
OWN - NLM
STAT- MEDLINE
DCOM- 20161020
LR  - 20181113
IS  - 1432-0983 (Electronic)
IS  - 0172-8083 (Linking)
VI  - 62
IP  - 1
DP  - 2016 Feb
TI  - Evolution and tinkering: what do a protein kinase, a transcriptional regulator 
      and chromosome segregation/cell division proteins have in common?
PG  - 67-70
LID - 10.1007/s00294-015-0513-y [doi]
AB  - In this study, we focus on functional interactions among multi-domain proteins 
      which share a common evolutionary origin. The examples we develop are four 
      Bacillus subtilis proteins, which all possess an ATP-binding Walker motif: the 
      bacterial tyrosine kinase (BY-kinase) PtkA, the chromosome segregation protein 
      Soj (ParA), the cell division protein MinD and a transcription regulator SalA. 
      These proteins have arisen via duplication of the ancestral ATP-binding domain, 
      which has undergone fusions with other functional domains in the process of 
      divergent evolution. We point out that these four proteins, despite having very 
      different physiological roles, engage in an unusually high number of binary 
      functional interactions. Namely, MinD attracts Soj and PtkA to the cell pole, and 
      in addition, activates the kinase function of PtkA. SalA also activates the 
      kinase function of PtkA, and it gets phosphorylated by PtkA as well. The 
      consequence of this phosphorylation is the activation of SalA as a 
      transcriptional repressor. We hypothesize that these functional interactions 
      remain preserved during divergent evolution and represent a constraint on the 
      process of evolutionary "tinkering", brought about by fusions of different 
      functional domains.
FAU - Derouiche, Abderahmane
AU  - Derouiche A
AD  - Systems and Synthetic Biology, Department of Biology and Biological Engineering, 
      Chalmers University of Technology, Goteborg, Sweden.
FAU - Shi, Lei
AU  - Shi L
AD  - Systems and Synthetic Biology, Department of Biology and Biological Engineering, 
      Chalmers University of Technology, Goteborg, Sweden.
FAU - Kalantari, Aida
AU  - Kalantari A
AD  - Systems and Synthetic Biology, Department of Biology and Biological Engineering, 
      Chalmers University of Technology, Goteborg, Sweden.
FAU - Mijakovic, Ivan
AU  - Mijakovic I
AD  - Systems and Synthetic Biology, Department of Biology and Biological Engineering, 
      Chalmers University of Technology, Goteborg, Sweden. ivan.mijakovic@chalmers.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20150819
PL  - United States
TA  - Curr Genet
JT  - Current genetics
JID - 8004904
RN  - 0 (Bacterial Proteins)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - EC 2.7.- (Protein Kinases)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Bacillus subtilis/genetics/metabolism
MH  - Bacterial Proteins/genetics/metabolism
MH  - *Biological Evolution
MH  - *Cell Division
MH  - *Chromosome Segregation
MH  - *Gene Expression Regulation
MH  - Protein Binding
MH  - Protein Interaction Domains and Motifs
MH  - Protein Kinases/chemistry/*metabolism
MH  - *Transcription, Genetic
OTO - NOTNLM
OT  - ATPase
OT  - Cell division
OT  - DNA-binding domain
OT  - Divergent evolution
OT  - Protein phosphorylation
OT  - Transcriptional regulation
EDAT- 2015/08/20 06:00
MHDA- 2016/10/21 06:00
CRDT- 2015/08/20 06:00
PHST- 2015/08/06 00:00 [received]
PHST- 2015/08/10 00:00 [accepted]
PHST- 2015/08/08 00:00 [revised]
PHST- 2015/08/20 06:00 [entrez]
PHST- 2015/08/20 06:00 [pubmed]
PHST- 2016/10/21 06:00 [medline]
AID - 10.1007/s00294-015-0513-y [pii]
AID - 10.1007/s00294-015-0513-y [doi]
PST - ppublish
SO  - Curr Genet. 2016 Feb;62(1):67-70. doi: 10.1007/s00294-015-0513-y. Epub 2015 Aug 
      19.