PMID- 26289744
OWN - NLM
STAT- MEDLINE
DCOM- 20151120
LR  - 20200315
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 48
IP  - 5
DP  - 2015 Oct
TI  - Role of growth hormone in maturation and activation of dendritic cells via 
      miR-200a and the Keap1/Nrf2 pathway.
PG  - 573-81
LID - 10.1111/cpr.12206 [doi]
AB  - OBJECTIVES: Dendritic cells (DCs) are antigen-presenting cells that participate 
      in the immune response; recently, it has been reported that growth hormone (GH) 
      promotes their maturation. The aim of this study was to investigate mechanisms by 
      which GH acts on DC maturation and activation. MATERIALS AND METHODS: Human 
      peripheral blood monocytes (HPBMs) were induced to become immature DCs and 
      treated with GH to obtain mature DCs. An osteosarcoma mouse model was established 
      by injection of LM8 cells to investigate anti-tumour effect of GH-induced DCs 
      in vivo. RESULTS: After administration of GH, DCs reduced miR-200a expression and 
      nuclear Nrf2 accumulation; miR-200a down-regulation inhibited DC maturation. Nrf2 
      ubiquitination level was increased by Keap1 overexpression in murine bone marrow 
      derived dendritic cells (BMDCs), which was cancelled by miR-200a in GH exposed 
      cells. In vivo, tumour volume was significantly reduced by GH-treated DCs and the 
      effect was reversed by overexpression of miR-200a. CONCLUSIONS: GH promoted 
      maturation and activation of DCs, and regulation of miR-200a played a part in 
      this process by modulation of the Keap1/Nrf2 pathway.
CI  - (c) 2015 John Wiley & Sons Ltd.
FAU - Liu, Qiu-Liang
AU  - Liu QL
AD  - Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052, China.
FAU - Zhang, Jiao
AU  - Zhang J
AD  - Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052, China.
FAU - Liu, Xin
AU  - Liu X
AD  - Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052, China.
FAU - Gao, Jing-Yao
AU  - Gao JY
AD  - Department of Pediatric Surgery, The First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, 450052, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150820
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (KEAP1 protein, human)
RN  - 0 (Kelch-Like ECH-Associated Protein 1)
RN  - 0 (MIRN200 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (RNA, Small Interfering)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*drug effects/immunology
MH  - Down-Regulation/drug effects
MH  - Female
MH  - Growth Hormone/*pharmacology
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/antagonists & 
      inhibitors/genetics/metabolism
MH  - Kelch-Like ECH-Associated Protein 1
MH  - Lung Neoplasms/secondary/therapy
MH  - Mice
MH  - Mice, Inbred C3H
MH  - MicroRNAs/antagonists & inhibitors/genetics/*metabolism
MH  - NF-E2-Related Factor 2/metabolism
MH  - Oligonucleotides, Antisense/genetics/metabolism
MH  - Osteoblastoma/pathology/therapy
MH  - RNA, Small Interfering/genetics/metabolism
MH  - Signal Transduction/*drug effects
MH  - Transplantation, Heterologous
MH  - Ubiquitination/drug effects
PMC - PMC6496568
COIS- All authors have no conflict of interest to state.
EDAT- 2015/08/21 06:00
MHDA- 2015/12/15 06:00
PMCR- 2015/08/20
CRDT- 2015/08/21 06:00
PHST- 2015/04/28 00:00 [received]
PHST- 2015/06/15 00:00 [accepted]
PHST- 2015/08/21 06:00 [entrez]
PHST- 2015/08/21 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
PHST- 2015/08/20 00:00 [pmc-release]
AID - CPR12206 [pii]
AID - 10.1111/cpr.12206 [doi]
PST - ppublish
SO  - Cell Prolif. 2015 Oct;48(5):573-81. doi: 10.1111/cpr.12206. Epub 2015 Aug 20.