PMID- 26293030
OWN - NLM
STAT- MEDLINE
DCOM- 20160401
LR  - 20191210
IS  - 1873-2623 (Electronic)
IS  - 0041-1345 (Linking)
VI  - 47
IP  - 6
DP  - 2015 Jul-Aug
TI  - Carnosol Is a Potent Lung Protective Agent: Experimental Study on Mice.
PG  - 1657-61
LID - S0041-1345(15)00518-7 [pii]
LID - 10.1016/j.transproceed.2015.05.004 [doi]
AB  - INTRODUCTION: Oxidative stress has been implicated in various disease states and 
      ischemia/reperfusion injury is a direct consequence of oxidative stress in lung 
      transplantation. Because the success rate of organ transplantation in which 
      ischemia/reperfusion is inevitable is highly influenced by oxidative stress, 
      development of strategies to control oxidative stress would be beneficial. Here 
      we identified natural compounds to reduce oxidative stresses in isolated mouse 
      lungs. METHODS: We screened compounds associated with antioxidative stress in 200 
      plant extracts by monitoring the activities of nuclear factor erythroid 2-related 
      factor 2 (NRF2). Compounds found to ameliorate antioxidative stress were enriched 
      and mice were administered the extract orally every day for 1 week. Then, the 
      lungs were isolated and cultured in the culture medium at 37  degrees C. Lung damage was 
      monitored by lactate dehydrogenase (LDH) released in the culture medium. Arterial 
      (left ventricle) blood gas levels were also monitored after hilar clamping. 
      RESULTS: We found that Callicarpa longissima extract was rich in NRF2 activators. 
      The responsible compounds were carnosic acid and its oxidative product, carnosol. 
      Carnosol induced heme-oxygenase 1 (HO-1) expression, which is downstream of NRF2, 
      more efficiently than carnosic acid. CONCLUSIONS: Lungs from mice treated with C 
      longissima extract were less damaged than those from control mice and accompanied 
      by HO-1 induction. These results suggest that carnosol is a candidate compound to 
      increase the success rate of lung transplantation.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Kawamura, T
AU  - Kawamura T
AD  - Department of General Thoracic Surgery, Osaka University Graduate School of 
      Medicine, Osaka, Japan; Laboratory of Cell Signaling & Metabolic Disease. 
      Electronic address: tkawamu@thoracic.med.osaka-u.ac.jp.
FAU - Momozane, T
AU  - Momozane T
AD  - Department of General Thoracic Surgery, Osaka University Graduate School of 
      Medicine, Osaka, Japan; Laboratory of Cell Signaling & Metabolic Disease.
FAU - Sanosaka, M
AU  - Sanosaka M
AD  - Laboratory of Cell Signaling & Metabolic Disease.
FAU - Sugimura, K
AU  - Sugimura K
AD  - Research Center for Medicinal Plant Resources, National Institute of Biomedical 
      Innovation, Osaka, Japan.
FAU - Iida, O
AU  - Iida O
AD  - Research Center for Medicinal Plant Resources, National Institute of Biomedical 
      Innovation, Osaka, Japan.
FAU - Fuchino, H
AU  - Fuchino H
AD  - Research Center for Medicinal Plant Resources, National Institute of Biomedical 
      Innovation, Osaka, Japan.
FAU - Funaki, S
AU  - Funaki S
AD  - Department of General Thoracic Surgery, Osaka University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Shintani, Y
AU  - Shintani Y
AD  - Department of General Thoracic Surgery, Osaka University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Inoue, M
AU  - Inoue M
AD  - Department of General Thoracic Surgery, Osaka University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Minami, M
AU  - Minami M
AD  - Department of General Thoracic Surgery, Osaka University Graduate School of 
      Medicine, Osaka, Japan.
FAU - Kawahara, N
AU  - Kawahara N
AD  - Research Center for Medicinal Plant Resources, National Institute of Biomedical 
      Innovation, Osaka, Japan.
FAU - Takemori, H
AU  - Takemori H
AD  - Laboratory of Cell Signaling & Metabolic Disease.
FAU - Okumura, M
AU  - Okumura M
AD  - Department of General Thoracic Surgery, Osaka University Graduate School of 
      Medicine, Osaka, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - 0 (Abietanes)
RN  - 0 (Antioxidants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Plant Extracts)
RN  - 483O455CKD (carnosol)
RN  - EC 1.1.- (Lactate Dehydrogenases)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - LI791SXT24 (salvin)
SB  - IM
MH  - Abietanes/*pharmacology
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Heme Oxygenase-1/metabolism
MH  - Lactate Dehydrogenases/metabolism
MH  - Lung/*drug effects/metabolism/pathology
MH  - Lung Injury/metabolism/pathology
MH  - Lung Transplantation/adverse effects
MH  - Male
MH  - Mice
MH  - NF-E2-Related Factor 2/metabolism
MH  - Oxidation-Reduction
MH  - Oxidative Stress/*drug effects
MH  - Plant Extracts/*pharmacology
MH  - Reperfusion Injury/metabolism/pathology
EDAT- 2015/08/22 06:00
MHDA- 2016/04/02 06:00
CRDT- 2015/08/22 06:00
PHST- 2015/02/15 00:00 [received]
PHST- 2015/05/11 00:00 [revised]
PHST- 2015/05/14 00:00 [accepted]
PHST- 2015/08/22 06:00 [entrez]
PHST- 2015/08/22 06:00 [pubmed]
PHST- 2016/04/02 06:00 [medline]
AID - S0041-1345(15)00518-7 [pii]
AID - 10.1016/j.transproceed.2015.05.004 [doi]
PST - ppublish
SO  - Transplant Proc. 2015 Jul-Aug;47(6):1657-61. doi: 
      10.1016/j.transproceed.2015.05.004.