PMID- 26296763
OWN - NLM
STAT- MEDLINE
DCOM- 20151215
LR  - 20200305
IS  - 1473-5644 (Electronic)
IS  - 0022-2615 (Linking)
VI  - 64
IP  - 9
DP  - 2015 Sep
TI  - SCCmec IX in meticillin-resistant Staphylococcus aureus and meticillin-resistant 
      coagulase-negative staphylococci from pigs and workers at pig farms in Khon Kaen, 
      Thailand.
PG  - 1087-1093
LID - 10.1099/jmm.0.000119 [doi]
AB  - Livestock-associated meticillin-resistant Staphylococcus aureus, clonal complex 
      (CC) 398, has been reported in Europe, whereas CC9 MRSA has mostly been found in 
      Asia. Therefore, we aimed to detect MRSA on pig farms in north-eastern Thailand. 
      A total of 257 nasal swabs (159 samples from pigs and 98 from pig-farm workers) 
      were collected from three pig farms in north-eastern Thailand from 2010 to 2011. 
      MRSA isolates were confirmed for femA and mecA genes by PCR. The MICs of eight 
      antimicrobials, namely vancomycin (VA), cefazolin (CZ), ofloxacin (OF), 
      tetracycline (TET), erythromycin (ER), oxacillin (OX), cefoxitin (FOX) and 
      gentamicin (GN), were tested by agar dilution method. The virulence genes for 
      Panton-Valentine leukocidin toxin (lukSF-PV), toxic shock syndrome toxin-1 (tst) 
      and alpha-haemolysin (hla) were detected by PCR. Strain typing was performed by 
      staphylococcal cassette chromosome (SCC) mec, agr, spa and multilocus sequence 
      typing. Four MRSA were isolated: three from workers and one from a pig. All the 
      MRSA isolates were resistant to OX, GN, ER, TET and CZ, and they all carried hla 
      only. Two MRSA from humans carried SCCmec II-sequence type (ST)764-agrII, whereas 
      the two remaining MRSA (one each from a human and a pig) contained SCCmec 
      IX-ST9-agrII. Interestingly, meticillin-resistant coagulase-negative 
      Staphylococcus isolates carrying SCCmec IX were also obtained from five workers 
      and three pigs. This study suggests that the SCCmec IX element is distributed 
      among the Staphylococcus found in pigs and pig-farm workers, and pigs may be a 
      reservoir for MRSA in the community.
FAU - Sinlapasorn, Siwaporn
AU  - Sinlapasorn S
AD  - Graduate School, Faculty of Associated Medical Sciences, Khon Kaen University, 
      Khon Kaen 40002, Thailand.
FAU - Lulitanond, Aroonlug
AU  - Lulitanond A
AD  - Centre for Research and Development of Medical Diagnostic Laboratories, Faculty 
      of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
FAU - Angkititrakul, Sunpetch
AU  - Angkititrakul S
AD  - Research Group for Preventive Technology in Livestock, Department of Veterinary 
      Public Health, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 
      40002, Thailand.
FAU - Chanawong, Aroonwadee
AU  - Chanawong A
AD  - Centre for Research and Development of Medical Diagnostic Laboratories, Faculty 
      of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
FAU - Wilailuckana, Chotechana
AU  - Wilailuckana C
AD  - Centre for Research and Development of Medical Diagnostic Laboratories, Faculty 
      of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
FAU - Tavichakorntrakool, Ratree
AU  - Tavichakorntrakool R
AD  - Centre for Research and Development of Medical Diagnostic Laboratories, Faculty 
      of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
FAU - Chindawong, Kanoksak
AU  - Chindawong K
AD  - Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Seelaget, Charinya
AU  - Seelaget C
AD  - Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Krasaesom, Mana
AU  - Krasaesom M
AD  - Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Chartchai, Sarawut
AU  - Chartchai S
AD  - Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
FAU - Wonglakorn, Lumyai
AU  - Wonglakorn L
AD  - Clinical Microbiology Laboratory, Srinagarind Hospital, Faculty of Medicine, Khon 
      Kaen University, Khon Kaen, Thailand.
FAU - Sribenjalux, Pipat
AU  - Sribenjalux P
AD  - Centre for Research and Development of Medical Diagnostic Laboratories, Faculty 
      of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150630
PL  - England
TA  - J Med Microbiol
JT  - Journal of medical microbiology
JID - 0224131
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - Q91FH1328A (Methicillin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Animals
MH  - Anti-Bacterial Agents/pharmacology
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Methicillin/pharmacology
MH  - Methicillin Resistance/*genetics
MH  - Methicillin-Resistant Staphylococcus aureus/genetics/*metabolism
MH  - Microbial Sensitivity Tests
MH  - Middle Aged
MH  - Occupational Exposure
MH  - Staphylococcal Infections/epidemiology/microbiology/*veterinary
MH  - Swine
MH  - Swine Diseases/epidemiology/*microbiology
MH  - Thailand/epidemiology
MH  - Young Adult
EDAT- 2015/08/25 06:00
MHDA- 2015/12/17 06:00
CRDT- 2015/08/23 06:00
PHST- 2015/08/23 06:00 [entrez]
PHST- 2015/08/25 06:00 [pubmed]
PHST- 2015/12/17 06:00 [medline]
AID - 10.1099/jmm.0.000119 [doi]
PST - ppublish
SO  - J Med Microbiol. 2015 Sep;64(9):1087-1093. doi: 10.1099/jmm.0.000119. Epub 2015 
      Jun 30.