PMID- 26299331
OWN - NLM
STAT- MEDLINE
DCOM- 20160719
LR  - 20240204
IS  - 0219-1032 (Electronic)
IS  - 1016-8478 (Print)
IS  - 1016-8478 (Linking)
VI  - 38
IP  - 9
DP  - 2015 Sep
TI  - LPS Up-Regulates ICAM-1 Expression in Breast Cancer Cells by Stimulating a 
      MyD88-BLT2-ERK-Linked Cascade, Which Promotes Adhesion to Monocytes.
PG  - 821-8
LID - 10.14348/molcells.2015.0174 [doi]
AB  - Monocytes are the major inflammatory cells that infiltrate most solid tumors in 
      humans. The interaction of tumor cells with infiltrating monocytes and their 
      adhesion to these monocytes play a significant role in altering the tumor to 
      become more aggressive. Recently, exposure to lipopolysaccharide (LPS) was 
      suggested to promote cancer cell adhesion to monocytes; however, little is known 
      about the details of the signaling mechanism involved in this process. In this 
      study, we found that LPS up-regulates ICAM-1 expression in MDA-MB-231 breast 
      cancer cells, which facilitates their adhesion to THP-1 monocytes. In addition, 
      we analyzed the signaling mechanism underlying the up-regulation of ICAM-1 and 
      found that the siRNA-mediated depletion of BLT2 markedly suppressed the 
      LPS-induced expression of ICAM-1 in MDA-MB-231 cells and the subsequent adhesion 
      of these cells to THP-1 monocytes. Moreover, we demonstrated that myeloid 
      differentiation primary response gene 88 (MyD88) lies downstream of LPS/TLR4 and 
      upstream of BLT2 and that this 'MyD88-BLT2' cascade mediates ERK activation and 
      subsequent ICAM-1 expression, which is critical for the adhesion of MDA-MB-231 
      cells to THP-1 monocytes. Taken together, our results demonstrate for the first 
      time that LPS up-regulates ICAM-1 expression in breast cancer cells via a 
      MyD88-BLT2-ERK-linked signaling cascade, leading to the increased adhesion of 
      breast cancer cells to monocytes.
FAU - Park, Geun-Soo
AU  - Park GS
AD  - School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, 
      Korea.
FAU - Kim, Jae-Hong
AU  - Kim JH
AD  - School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, 
      Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150821
PL  - United States
TA  - Mol Cells
JT  - Molecules and cells
JID - 9610936
RN  - 0 (ICAM1 protein, human)
RN  - 0 (LTB4R2 protein, human)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (MYD88 protein, human)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Receptors, Leukotriene B4)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Breast Neoplasms
MH  - Cell Adhesion/drug effects
MH  - Cell Line, Tumor
MH  - Coculture Techniques
MH  - Female
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/genetics/*metabolism
MH  - Lipopolysaccharides/*pharmacology
MH  - MAP Kinase Signaling System
MH  - Monocytes/immunology/*metabolism
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - Receptors, Leukotriene B4/metabolism
MH  - Up-Regulation
PMC - PMC4588726
OTO - NOTNLM
OT  - BLT2
OT  - ICAM-1
OT  - LPS
OT  - MyD88
OT  - beast cancer
EDAT- 2015/08/25 06:00
MHDA- 2016/07/20 06:00
PMCR- 2015/08/21
CRDT- 2015/08/25 06:00
PHST- 2015/06/16 00:00 [received]
PHST- 2015/07/17 00:00 [revised]
PHST- 2015/07/17 00:00 [accepted]
PHST- 2015/08/25 06:00 [entrez]
PHST- 2015/08/25 06:00 [pubmed]
PHST- 2016/07/20 06:00 [medline]
PHST- 2015/08/21 00:00 [pmc-release]
AID - S1016-8478(23)05496-1 [pii]
AID - molce-38-9-821 [pii]
AID - 10.14348/molcells.2015.0174 [doi]
PST - ppublish
SO  - Mol Cells. 2015 Sep;38(9):821-8. doi: 10.14348/molcells.2015.0174. Epub 2015 Aug 
      21.