PMID- 26300874
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150824
LR  - 20201001
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 6
DP  - 2015
TI  - Comparative genomic analysis of multiple strains of two unusual plant pathogens: 
      Pseudomonas corrugata and Pseudomonas mediterranea.
PG  - 811
LID - 10.3389/fmicb.2015.00811 [doi]
LID - 811
AB  - The non-fluorescent pseudomonads, Pseudomonas corrugata (Pcor) and P. 
      mediterranea (Pmed), are closely related species that cause pith necrosis, a 
      disease of tomato that causes severe crop losses. However, they also show strong 
      antagonistic effects against economically important pathogens, demonstrating 
      their potential for utilization as biological control agents. In addition, their 
      metabolic versatility makes them attractive for the production of commercial 
      biomolecules and bioremediation. An extensive comparative genomics study is 
      required to dissect the mechanisms that Pcor and Pmed employ to cause disease, 
      prevent disease caused by other pathogens, and to mine their genomes for genes 
      that encode proteins involved in commercially important chemical pathways. Here, 
      we present the draft genomes of nine Pcor and Pmed strains from different 
      geographical locations. This analysis covered significant genetic heterogeneity 
      and allowed in-depth genomic comparison. All examined strains were able to 
      trigger symptoms in tomato plants but not all induced a hypersensitive-like 
      response in Nicotiana benthamiana. Genome-mining revealed the absence of type III 
      secretion system and known type III effector-encoding genes from all examined 
      Pcor and Pmed strains. The lack of a type III secretion system appears to be 
      unique among the plant pathogenic pseudomonads. Several gene clusters coding for 
      type VI secretion system were detected in all genomes. Genome-mining also 
      revealed the presence of gene clusters for biosynthesis of siderophores, 
      polyketides, non-ribosomal peptides, and hydrogen cyanide. A highly conserved 
      quorum sensing system was detected in all strains, although species specific 
      differences were observed. Our study provides the basis for in-depth 
      investigations regarding the molecular mechanisms underlying virulence strategies 
      in the battle between plants and microbes.
FAU - Trantas, Emmanouil A
AU  - Trantas EA
AD  - Plant Biochemistry and Biotechnology Laboratory, Department of Agriculture, 
      School of Agriculture and Food Technology, Technological Educational Institute of 
      Crete Heraklion, Greece.
FAU - Licciardello, Grazia
AU  - Licciardello G
AD  - Science and Technology Park of Sicily Catania, Italy.
FAU - Almeida, Nalvo F
AU  - Almeida NF
AD  - School of Computing, Federal University of Mato Grosso do Sul Campo Grande, 
      Brazil.
FAU - Witek, Kamil
AU  - Witek K
AD  - The Sainsbury Laboratory, John Innes Centre Norwich, UK.
FAU - Strano, Cinzia P
AU  - Strano CP
AD  - Department of Agriculture, Food and Environment, University of Catania Catania, 
      Italy.
FAU - Duxbury, Zane
AU  - Duxbury Z
AD  - The Sainsbury Laboratory, John Innes Centre Norwich, UK.
FAU - Ververidis, Filippos
AU  - Ververidis F
AD  - Plant Biochemistry and Biotechnology Laboratory, Department of Agriculture, 
      School of Agriculture and Food Technology, Technological Educational Institute of 
      Crete Heraklion, Greece.
FAU - Goumas, Dimitrios E
AU  - Goumas DE
AD  - Plant Biochemistry and Biotechnology Laboratory, Department of Agriculture, 
      School of Agriculture and Food Technology, Technological Educational Institute of 
      Crete Heraklion, Greece ; Plant Pathology and Bacteriology Laboratory, Department 
      of Agriculture, School of Agriculture and Food Technology, Technological 
      Educational Institute of Crete Heraklion, Greece.
FAU - Jones, Jonathan D G
AU  - Jones JD
AD  - The Sainsbury Laboratory, John Innes Centre Norwich, UK.
FAU - Guttman, David S
AU  - Guttman DS
AD  - Centre for the Analysis of Genome Evolution & Function, University of Toronto 
      Toronto, ON, Canada.
FAU - Catara, Vittoria
AU  - Catara V
AD  - Department of Agriculture, Food and Environment, University of Catania Catania, 
      Italy.
FAU - Sarris, Panagiotis F
AU  - Sarris PF
AD  - Plant Biochemistry and Biotechnology Laboratory, Department of Agriculture, 
      School of Agriculture and Food Technology, Technological Educational Institute of 
      Crete Heraklion, Greece ; The Sainsbury Laboratory, John Innes Centre Norwich, 
      UK.
LA  - eng
PT  - Journal Article
DEP - 20150807
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC4528175
OTO - NOTNLM
OT  - comparative genomics
OT  - effectors
OT  - non-ribosomal peptides
OT  - pith necrosis
OT  - polyketides
OT  - siderophores
OT  - type III secretion system
OT  - type VI secretion system
EDAT- 2015/08/25 06:00
MHDA- 2015/08/25 06:01
PMCR- 2015/08/07
CRDT- 2015/08/25 06:00
PHST- 2015/04/23 00:00 [received]
PHST- 2015/07/22 00:00 [accepted]
PHST- 2015/08/25 06:00 [entrez]
PHST- 2015/08/25 06:00 [pubmed]
PHST- 2015/08/25 06:01 [medline]
PHST- 2015/08/07 00:00 [pmc-release]
AID - 10.3389/fmicb.2015.00811 [doi]
PST - epublish
SO  - Front Microbiol. 2015 Aug 7;6:811. doi: 10.3389/fmicb.2015.00811. eCollection 
      2015.