PMID- 26302057
OWN - NLM
STAT- MEDLINE
DCOM- 20160418
LR  - 20181113
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Print)
IS  - 0019-2805 (Linking)
VI  - 146
IP  - 3
DP  - 2015 Nov
TI  - Protection against Paracoccidioides brasiliensis infection in mice treated with 
      modulated dendritic cells relies on inhibition of interleukin-10 production by 
      CD8+ T cells.
PG  - 486-95
LID - 10.1111/imm.12526 [doi]
AB  - Paracoccidioidomycosis is a systemic infection prevalent in Latin American 
      countries. Disease develops after inhalation of Paracoccidioides brasiliensis 
      conidia followed by an improper immune activation by the host leucocytes. 
      Dendritic cells (DCs) are antigen-presenting cells with the unique ability to 
      direct the adaptive immune response by the time of activation of naive T cells. 
      This study was conducted to test whether extracts of P. brasiliensis would induce 
      maturation of DCs. We found that DCs treated with extracts acquired an 
      inflammatory phenotype and upon adoptive transfer conferred protection to 
      infection. Interestingly, interleukin-10 production by CD8(+) T cells was ablated 
      following DC transfer. Further analyses showed that lymphocytes from infected 
      mice were high producers of interleukin-10, with CD8(+) T cells being the main 
      source. Blockage of cross-presentation to CD8(+) T cells by modulated DCs 
      abolished the protective effect of adoptive transfer. Collectively, our data show 
      that adoptive transfer of P. brasiliensis-modulated DCs is an interesting 
      approach for the control of infection in paracoccidioidomycosis.
CI  - (c) 2015 John Wiley & Sons Ltd.
FAU - da Costa, Thiago Alves
AU  - da Costa TA
AD  - Department of Structural and Functional Biology, Institute of Biology, University 
      of Campinas, Campinas, SP, Brazil.
FAU - Di Gangi, Rosaria
AU  - Di Gangi R
AD  - Department of Structural and Functional Biology, Institute of Biology, University 
      of Campinas, Campinas, SP, Brazil.
FAU - Martins, Paula
AU  - Martins P
AD  - Department of Structural and Functional Biology, Institute of Biology, University 
      of Campinas, Campinas, SP, Brazil.
FAU - Longhini, Ana Leda Figueiredo
AU  - Longhini AL
AD  - Department of Haematology, Faculdade de Ciencias Medicas, University of Campinas, 
      Campinas, SP, Brazil.
FAU - Zanucoli, Fabio
AU  - Zanucoli F
AD  - Department of Structural and Functional Biology, Institute of Biology, University 
      of Campinas, Campinas, SP, Brazil.
FAU - de Oliveira, Alexandre Leite Rodrigues
AU  - de Oliveira AL
AD  - Department of Structural and Functional Biology, Institute of Biology, University 
      of Campinas, Campinas, SP, Brazil.
FAU - Stach-Machado, Dagmar Ruth
AU  - Stach-Machado DR
AD  - Department of Structural and Functional Biology, Institute of Biology, University 
      of Campinas, Campinas, SP, Brazil.
FAU - Burger, Eva
AU  - Burger E
AD  - Instituto de Biociencias, Universidade Federal de Alfenas, Alfenas, MG, Brazil.
FAU - Verinaud, Liana
AU  - Verinaud L
AD  - Department of Structural and Functional Biology, Institute of Biology, University 
      of Campinas, Campinas, SP, Brazil.
FAU - Thome, Rodolfo
AU  - Thome R
AD  - Department of Structural and Functional Biology, Institute of Biology, University 
      of Campinas, Campinas, SP, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150921
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antigens, Fungal)
RN  - 0 (Cytokines)
RN  - 0 (Fungal Vaccines)
RN  - 0 (IL10 protein, mouse)
RN  - 0 (Inflammation Mediators)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Antigens, Fungal/pharmacology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cell Differentiation/immunology
MH  - Cross-Priming
MH  - Cytokines/biosynthesis
MH  - Dendritic Cells/cytology/*immunology/microbiology
MH  - Female
MH  - Fungal Vaccines/immunology/pharmacology
MH  - Inflammation Mediators/metabolism
MH  - Interleukin-10/antagonists & inhibitors/*biosynthesis
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Paracoccidioides/*immunology
MH  - Paracoccidioidomycosis/*immunology/*prevention & control
PMC - PMC4610636
OTO - NOTNLM
OT  - Paracoccidioides brasiliensis
OT  - cell-based vaccine
OT  - dendritic cells
OT  - fungal infection
OT  - immunoregulation
OT  - paracoccidioidomycosis
EDAT- 2015/08/25 06:00
MHDA- 2016/04/19 06:00
PMCR- 2016/11/01
CRDT- 2015/08/25 06:00
PHST- 2015/04/22 00:00 [received]
PHST- 2015/08/08 00:00 [revised]
PHST- 2015/08/18 00:00 [accepted]
PHST- 2015/08/25 06:00 [entrez]
PHST- 2015/08/25 06:00 [pubmed]
PHST- 2016/04/19 06:00 [medline]
PHST- 2016/11/01 00:00 [pmc-release]
AID - 10.1111/imm.12526 [doi]
PST - ppublish
SO  - Immunology. 2015 Nov;146(3):486-95. doi: 10.1111/imm.12526. Epub 2015 Sep 21.