PMID- 26303819
OWN - NLM
STAT- MEDLINE
DCOM- 20160624
LR  - 20181202
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 119
IP  - 5
DP  - 2015 Nov
TI  - Difference of microbial community stressed in artificial pit muds for 
      Luzhou-flavour liquor brewing revealed by multiphase culture-independent 
      technology.
PG  - 1345-56
LID - 10.1111/jam.12943 [doi]
AB  - AIMS: Artificial pit muds (APMs) is produced by peats, aged pit muds, yellow and 
      black clays etc. and is one of essential factors for Luzhou-flavour liquor 
      production. The microbial community of APMs significantly influence the quality 
      of Luzhou-flavour liquor. The aim of this study was to investigate the 
      differences in bacterial, archaeal and fungal community of APMs, starters and 
      materials. METHODS AND RESULTS: Multiphase culture-independent technology were 
      employed in this study, including nested PCR-denaturing gradient gel 
      electrophoresis (nested PCR-DGGE), phospholipid fatty acid (PLFA), phospholipid 
      ether lipids (PLEL) and fluorescence in situ hybridization (FISH) analysis. 
      Results suggested that the microbial diversity significantly changed under 
      environmental stress and different culture patterns during APMs cultivation. The 
      dominant bacteria in APMs mainly fell into Clostridiales, Lactobacillales, 
      Bacteroidales and Rhizobiales, Archaea affiliated with Methanomicrobiales and 
      Methanosarcinales, and fungi belonged to Saccharomycetales and Eurotiales. 
      Furthermore, the microbial community structures of APMs cultured by ground pile 
      pattern were more similar with that of aged pit muds, meanwhile, the relative 
      bands intensities of microbes, which are the main contributors for liquor 
      brewing, increased with the culture times. CONCLUSIONS: Not only the niche 
      selection and biogeochemical properties of APMs, but also the mutual 
      collaboration and constraint between different microbes may result in enriching 
      different liquor-brewing microbes into APMs. SIGNIFICANCE AND IMPACT OF THE 
      STUDY: APM cultivation technology was necessary to promote enriching functional 
      liquor-brewing microbes into APMs. These results may facilitate understanding the 
      microbial succession during APMs manufacture.
CI  - (c) 2015 The Society for Applied Microbiology.
FAU - Zhang, L
AU  - Zhang L
AD  - College of Light Industry, Textile & Food Engineering, Sichuan University, 
      Chengdu, China.
AD  - Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, 
      Sichuan University, Chengdu, China.
FAU - Zhou, R
AU  - Zhou R
AD  - College of Light Industry, Textile & Food Engineering, Sichuan University, 
      Chengdu, China.
AD  - Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, 
      Sichuan University, Chengdu, China.
AD  - National Engineering Research Centre of Solid-State Brewing, Luzhou, China.
FAU - Niu, M
AU  - Niu M
AD  - College of Light Industry, Textile & Food Engineering, Sichuan University, 
      Chengdu, China.
AD  - Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, 
      Sichuan University, Chengdu, China.
FAU - Zheng, J
AU  - Zheng J
AD  - College of Light Industry, Textile & Food Engineering, Sichuan University, 
      Chengdu, China.
AD  - Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, 
      Sichuan University, Chengdu, China.
FAU - Wu, C
AU  - Wu C
AD  - College of Light Industry, Textile & Food Engineering, Sichuan University, 
      Chengdu, China.
AD  - Key Laboratory for Leather Chemistry and Engineering of the Education Ministry, 
      Sichuan University, Chengdu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151012
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (Aluminum Silicates)
RN  - T1FAD4SS2M (Clay)
SB  - IM
MH  - Alcoholic Beverages/*microbiology
MH  - Aluminum Silicates/*chemistry
MH  - Archaea/classification/genetics/growth & development/*isolation & purification
MH  - Bacteria/classification/genetics/growth & development/*isolation & purification
MH  - Clay
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Fermentation
MH  - Fungi/classification/genetics/growth & development/*isolation & purification
MH  - In Situ Hybridization, Fluorescence
MH  - Polymerase Chain Reaction
OTO - NOTNLM
OT  - FISH analysis
OT  - PCR-DGGE
OT  - PLFA and PLEL analysis
OT  - artificial pit muds
OT  - microbial community
EDAT- 2015/08/26 06:00
MHDA- 2016/06/25 06:00
CRDT- 2015/08/26 06:00
PHST- 2015/06/16 00:00 [received]
PHST- 2015/08/05 00:00 [revised]
PHST- 2015/08/14 00:00 [accepted]
PHST- 2015/08/26 06:00 [entrez]
PHST- 2015/08/26 06:00 [pubmed]
PHST- 2016/06/25 06:00 [medline]
AID - 10.1111/jam.12943 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2015 Nov;119(5):1345-56. doi: 10.1111/jam.12943. Epub 2015 Oct 
      12.