PMID- 26304353 OWN - NLM STAT- MEDLINE DCOM- 20160425 LR - 20181202 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 192 DP - 2016 Feb 1 TI - Quantitative analysis of amoxicillin, its major metabolites and ampicillin in eggs by liquid chromatography combined with electrospray ionization tandem mass spectrometry. PG - 313-8 LID - S0308-8146(15)01033-X [pii] LID - 10.1016/j.foodchem.2015.07.028 [doi] AB - In this present study, we developed a simple, rapid and specific method for the quantitative analysis of the contents of amoxicillin (AMO), AMO metabolites and ampicillin (AMP) in eggs. This method uses a simple liquid-liquid extraction with acetonitrile followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The optimized method has been validated according to requirements defined by the European Union and Food and Drug Administration. Extraction recoveries of the target compounds from the egg at 5, 10 and 25 mug/kg were all higher than 80%, with relative standard deviations not exceeding 10.00%. The limits of quantification in eggs were below the maximum residue limits (MRLs). The decision limits (CCalpha) ranged between 11.1 and 11.5 mug/kg, while detection capabilities (CCbeta) from 12.1 to 13.0 mug/kg. These values were very close to the corresponding MRLs. Finally, the new approach was successfully verified for the quantitative determination of these analytes in 40 commercial eggs from local supermarkets. CI - Copyright (c) 2015 Elsevier Ltd. All rights reserved. FAU - Sun, Lirui AU - Sun L AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China. FAU - Jia, Longfei AU - Jia L AD - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People's Republic of China. FAU - Xie, Xing AU - Xie X AD - College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, People's Republic of China. FAU - Xie, Kaizhou AU - Xie K AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China. Electronic address: yzxkz168@163.com. FAU - Wang, Jianfeng AU - Wang J AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China. FAU - Liu, Jianyu AU - Liu J AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China. FAU - Cui, Lulu AU - Cui L AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China. FAU - Zhang, Genxi AU - Zhang G AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China. FAU - Dai, Guojun AU - Dai G AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China. FAU - Wang, Jinyu AU - Wang J AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, People's Republic of China; Key Laboratory for Animal Genetic, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou 225009, People's Republic of China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150708 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 RN - 0 (Acetonitriles) RN - 0 (Veterinary Drugs) RN - 7C782967RD (Ampicillin) RN - 804826J2HU (Amoxicillin) RN - Z072SB282N (acetonitrile) SB - IM MH - Acetonitriles/chemistry MH - Amoxicillin/*analysis/metabolism MH - Ampicillin/analysis/metabolism MH - Animals MH - Chromatography, Liquid/*methods MH - Eggs/*analysis MH - Food Contamination/*analysis MH - Liquid-Liquid Extraction MH - Reproducibility of Results MH - Spectrometry, Mass, Electrospray Ionization/*methods MH - Tandem Mass Spectrometry/*methods MH - United States MH - Veterinary Drugs/*analysis/metabolism OTO - NOTNLM OT - Acetonitrile (PubChem CID:6342) OT - Ammonium acetate (PubChem CID:517165) OT - Amoxicillin OT - Amoxicillin (PubChem CID:33613) OT - Amoxicillin diketopiperazine-2',5'-dione OT - Amoxicillin diketopiperazine-2',5'-dione (PubChem CID:46780379) OT - Amoxicilloic acid OT - Amoxicilloic acid (PubChem CID:71313389) OT - Ampicillin OT - Ampicillin (PubChem CID:6249) OT - Glacial acetic acid (PubChem CID:176) OT - LC-MS/MS OT - Penicillin V (PubChem CID:6869) OT - Residues in eggs EDAT- 2015/08/26 06:00 MHDA- 2016/04/26 06:00 CRDT- 2015/08/26 06:00 PHST- 2014/04/19 00:00 [received] PHST- 2015/04/27 00:00 [revised] PHST- 2015/07/07 00:00 [accepted] PHST- 2015/08/26 06:00 [entrez] PHST- 2015/08/26 06:00 [pubmed] PHST- 2016/04/26 06:00 [medline] AID - S0308-8146(15)01033-X [pii] AID - 10.1016/j.foodchem.2015.07.028 [doi] PST - ppublish SO - Food Chem. 2016 Feb 1;192:313-8. doi: 10.1016/j.foodchem.2015.07.028. Epub 2015 Jul 8.