PMID- 26305287
OWN - NLM
STAT- MEDLINE
DCOM- 20160617
LR  - 20211203
IS  - 1607-8888 (Electronic)
IS  - 1025-3890 (Print)
IS  - 1025-3890 (Linking)
VI  - 18
IP  - 6
DP  - 2015
TI  - Long-term effects of early-life caregiving experiences on brain-derived 
      neurotrophic factor histone acetylation in the adult rat mPFC.
PG  - 607-15
LID - 10.3109/10253890.2015.1071790 [doi]
AB  - Infant-caregiver experiences are major contributing factors to neural and 
      behavioral development. Research indicates that epigenetic mechanisms provide a 
      way in which infant-caregiver experiences affect gene activity and other 
      downstream processes in the brain that influence behavioral development. Our 
      laboratory previously demonstrated in a rodent model that exposure to 
      maltreatment alters methylation of DNA associated with the brain-derived 
      neurotrophic factor (bdnf) and reelin genes as well as mRNA of key epigenetic 
      regulatory genes in the medial prefrontal cortex (mPFC). In the current study, we 
      characterized patterns of histone acetylation at bdnf and reelin gene loci after 
      our caregiver manipulations. Using a within-litter design (n = 8-10/group from 
      eight litters), pups were exposed to adverse (maltreatment condition: exposure to 
      a stressed caregiver) or nurturing (cross-foster condition: exposure to a 
      nurturing caregiver) caregiving environments outside the home cage for 30 min 
      daily during the first postnatal week. Remaining pups in a litter were left with 
      the biological mother during each session (providing normal care controls). We 
      then used chromatin immunoprecipitation (ChIP) and quantitative RT-PCR to measure 
      histone 3 lysine 9/14 acetylation associated with bdnf promoters I and IV and the 
      reelin promoter in the adult mPFC. Maltreated females had decreased acetylation 
      at bdnf IV, while neither males nor females exhibited histone acetylation 
      alterations at bdnf I or reelin. These data demonstrate the ability of 
      maltreatment to have long-term consequences on histone acetylation in the mPFC, 
      and provide further evidence of the epigenetic susceptibility of bdnf IV to the 
      quality of infant-caregiver experiences.
FAU - Blaze, Jennifer
AU  - Blaze J
AD  - a Department of Psychological and Brain Sciences , University of Delaware , 
      Newark , DE , USA.
FAU - Asok, Arun
AU  - Asok A
AD  - a Department of Psychological and Brain Sciences , University of Delaware , 
      Newark , DE , USA.
FAU - Roth, Tania L
AU  - Roth TL
AD  - a Department of Psychological and Brain Sciences , University of Delaware , 
      Newark , DE , USA.
LA  - eng
GR  - P20 GM103653/GM/NIGMS NIH HHS/United States
GR  - 1P20GM103653/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20150818
PL  - England
TA  - Stress
JT  - Stress (Amsterdam, Netherlands)
JID - 9617529
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cell Adhesion Molecules, Neuronal)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Histones)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reelin Protein)
RN  - 0 (Reln protein, rat)
RN  - EC 3.4.21.- (Serine Endopeptidases)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cell Adhesion Molecules, Neuronal/genetics/metabolism
MH  - *Epigenesis, Genetic
MH  - Extracellular Matrix Proteins/genetics/metabolism
MH  - Female
MH  - Histones/genetics/*metabolism
MH  - Male
MH  - Maternal Behavior
MH  - Nerve Tissue Proteins/genetics/metabolism
MH  - Prefrontal Cortex/*metabolism
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Reelin Protein
MH  - Serine Endopeptidases/genetics/metabolism
PMC - PMC4879775
MID - NIHMS787835
OTO - NOTNLM
OT  - Development
OT  - H3K9
OT  - epigenetic
OT  - maltreatment
OT  - maternal
OT  - reelin
COIS- Declaration of Interest The authors report no conflicts of interest. This work 
      was supported by a grant from the National Institute of General Medical Sciences 
      (1P20GM103653).
EDAT- 2015/08/26 06:00
MHDA- 2016/06/18 06:00
PMCR- 2016/05/25
CRDT- 2015/08/26 06:00
PHST- 2015/08/26 06:00 [entrez]
PHST- 2015/08/26 06:00 [pubmed]
PHST- 2016/06/18 06:00 [medline]
PHST- 2016/05/25 00:00 [pmc-release]
AID - 10.3109/10253890.2015.1071790 [doi]
PST - ppublish
SO  - Stress. 2015;18(6):607-15. doi: 10.3109/10253890.2015.1071790. Epub 2015 Aug 18.