PMID- 26305415
OWN - NLM
STAT- MEDLINE
DCOM- 20160325
LR  - 20191210
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 87
IP  - 18
DP  - 2015 Sep 15
TI  - Portable kit for identification and detection of drugs in human urine using 
      surface-enhanced Raman spectroscopy.
PG  - 9500-6
LID - 10.1021/acs.analchem.5b02899 [doi]
AB  - A portable kit was demonstrated for rapid and reliable surface-enhanced Raman 
      scattering (SERS) detection of drugs in human urine. This kit contains two sealed 
      reagent tubes, a packet of standardized SERS substrates, and a mini Raman device. 
      A 3 min pretreatment for separating amphetamines from human urine was developed 
      with an extraction rate of >80% examined by ultraperformance liquid 
      chromatography (UPLC). Simultaneously, highly reproducible two-dimensional (2D) 
      gold nanorod (GNR) arrays were assembled by the use of 
      methoxymercaptopoly(ethylene glycol) (mPEG-SH) capping. Thirty batches of GNR 
      arrays produced the 1001 cm(-1) intensity of methamphetamine (MA) molecules with 
      a relative standard deviation (RSD) of 7.9%, and a 21 x 21 mum(2) area mapping on 
      a 2D GNR array produced a statistical RSD of <10%, implying an excellent 
      reproducibility and uniformity. The detection limit of amphetamines in human 
      urine was at least 0.1 ppm. Moreover, the portable kit was successfully used for 
      detecting MA, 3,4-methylenedioxymethamphetamine (MDMA), and methcathinone (MC) in 
      30 volunteers' urine samples with various clinical natures, and the dual-analyte 
      detection of MA and MDMA implied a good capability of multiplex analysis. UPLC 
      examination and the SERS recovery test clearly indicated that our pretreatment 
      procedure was sufficient to lower the high background signals caused by complex 
      components in urine and demonstrated the practicability and the resistance to 
      false positives, which is a vital problem for law enforcement applications. The 
      excellent performance of our portable kit promises a great prospective toward a 
      rapid, reliable, and on-spot analyzer, especially for public safety and 
      healthcare.
FAU - Han, Zhenzhen
AU  - Han Z
AD  - Institute of Intelligent Machines and double daggerCancer Hospital, Hefei Institutes of 
      Physical Science , Chinese Academy of Sciences, Hefei 230031, China.
FAU - Liu, Honglin
AU  - Liu H
AD  - Institute of Intelligent Machines and double daggerCancer Hospital, Hefei Institutes of 
      Physical Science , Chinese Academy of Sciences, Hefei 230031, China.
FAU - Meng, Juan
AU  - Meng J
AD  - Institute of Intelligent Machines and double daggerCancer Hospital, Hefei Institutes of 
      Physical Science , Chinese Academy of Sciences, Hefei 230031, China.
FAU - Yang, Liangbao
AU  - Yang L
AD  - Institute of Intelligent Machines and double daggerCancer Hospital, Hefei Institutes of 
      Physical Science , Chinese Academy of Sciences, Hefei 230031, China.
FAU - Liu, Jing
AU  - Liu J
AD  - Institute of Intelligent Machines and double daggerCancer Hospital, Hefei Institutes of 
      Physical Science , Chinese Academy of Sciences, Hefei 230031, China.
FAU - Liu, Jinhuai
AU  - Liu J
AD  - Institute of Intelligent Machines and double daggerCancer Hospital, Hefei Institutes of 
      Physical Science , Chinese Academy of Sciences, Hefei 230031, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150901
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Illicit Drugs)
RN  - 0 (Propiophenones)
RN  - 386QA522QG (monomethylpropion)
RN  - 7440-57-5 (Gold)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - Feasibility Studies
MH  - Gold/chemistry
MH  - Humans
MH  - Illicit Drugs/*urine
MH  - Limit of Detection
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*urine
MH  - Nanotubes/chemistry
MH  - Propiophenones/*urine
MH  - Reproducibility of Results
MH  - *Spectrum Analysis, Raman
MH  - Surface Properties
MH  - Time Factors
MH  - Urinalysis/*instrumentation
EDAT- 2015/08/26 06:00
MHDA- 2016/03/26 06:00
CRDT- 2015/08/26 06:00
PHST- 2015/08/26 06:00 [entrez]
PHST- 2015/08/26 06:00 [pubmed]
PHST- 2016/03/26 06:00 [medline]
AID - 10.1021/acs.analchem.5b02899 [doi]
PST - ppublish
SO  - Anal Chem. 2015 Sep 15;87(18):9500-6. doi: 10.1021/acs.analchem.5b02899. Epub 
      2015 Sep 1.