PMID- 26305422
OWN - NLM
STAT- MEDLINE
DCOM- 20160726
LR  - 20191210
IS  - 1502-7686 (Electronic)
IS  - 0036-5513 (Linking)
VI  - 75
IP  - 7
DP  - 2015 Nov
TI  - Hypo- and hypernatremia results in inaccurate erythrocyte mean corpuscular volume 
      measurement in vitro, when using Sysmex XE 2100.
PG  - 588-94
LID - 10.3109/00365513.2015.1062534 [doi]
AB  - INTRODUCTION: Automated hematology analyzers dilute patient erythrocytes with an 
      isoosmotic diluent before quantitating the erythrocyte mean cell volume (MCV). 
      However, if patient plasma osmolality differs from the diluent, water will cross 
      the erythrocytes membrane and establish a new equilibrium across the membrane. 
      Since the new equilibrium is reached before the measurement of the MCV, the 
      measured MCV may not reflect the true MCV in vivo. AIM: Calculation of the 
      theoretical change in MCV at changed P-Sodium/P-Osmolality and to investigate if 
      the automated blood cell counter Sysmex XE 2100 measures MCV correctly in hypo- 
      and hyperosmolality and hypo-and hypernatremia. In addition, to examine whether 
      the theoretically calculated change in MCV corresponds with the experimentally 
      determined MCV change. METHOD: Theoretical calculation of the MCV inaccuracy at 
      hypo- and hypernatremia, as well as at hypo- and hyperosmolality. Experimental 
      studies with comparison of MCV measured at Sysmex XE 2100 to MCV found by using 
      the manual measured packed cell volume method. RESULTS AND CONCLUSION: 
      Measurement of MCV in hypo- and hypernatremia patients using the automated blood 
      cell counter Sysmex XE 2100 resulted in inaccurate MCV. The experimental results 
      also revealed a strong correlation between P-Osmolality/P-Sodium and MCV 
      inaccuracy (R(2) = 0.70/0.85) similar to the theoretically calculated MCV 
      inaccuracy. We suggest using mean cellular Hb (MCH) instead of MCV, mean 
      corpuscular Hb concentration (MCHC) and B-Erythrocyte volume fraction (EVF). 
      Alternatively, we suggest standardizing the measured MCV to a normal P-Sodium 
      e.g. 140 mmol/L to estimate the in vivo MCV.
FAU - Philipsen, Jens Peter
AU  - Philipsen JP
AD  - a Nordsjaellands Hospital, Biomedical Laboratory , Hillerod , Denmark.
FAU - Madsen, Kirsten Vikkelso
AU  - Madsen KV
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20150814
PL  - England
TA  - Scand J Clin Lab Invest
JT  - Scandinavian journal of clinical and laboratory investigation
JID - 0404375
SB  - IM
MH  - *Erythrocyte Indices
MH  - Female
MH  - Hematologic Tests/*standards
MH  - Humans
MH  - Hypernatremia/blood/*complications
MH  - Hyponatremia/blood/*complications
MH  - Male
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Anemias
OT  - cell size
OT  - erythrocytes
OT  - hematology
OT  - hyponatremia
EDAT- 2015/08/26 06:00
MHDA- 2016/07/28 06:00
CRDT- 2015/08/26 06:00
PHST- 2015/08/26 06:00 [entrez]
PHST- 2015/08/26 06:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
AID - 10.3109/00365513.2015.1062534 [doi]
PST - ppublish
SO  - Scand J Clin Lab Invest. 2015 Nov;75(7):588-94. doi: 
      10.3109/00365513.2015.1062534. Epub 2015 Aug 14.