PMID- 26308756
OWN - NLM
STAT- MEDLINE
DCOM- 20151001
LR  - 20150828
IS  - 1078-6791 (Print)
IS  - 1078-6791 (Linking)
VI  - 21 Suppl 2
DP  - 2015
TI  - Hepatoprotective Effects of Grape Seed Procyanidin B2 in Rats With Carbon 
      Tetrachloride-induced Hepatic Fibrosis.
PG  - 12-21
AB  - CONTEXT: Infectious hepatitis is a serious problem affecting millions of people 
      worldwide, particularly in China and other developing countries, and it is the 
      major risk factor for hepatic cirrhosis. To date, the pathogenesis of hepatic 
      cirrhosis is complex and unclear. Traditional Chinese medicine (TCM) has long 
      been used in its treatment; however, little is known to date about the effects of 
      grape seed procyanidin B2 (GSPB2) on liver fibrosis. OBJECTIVES: Using a rat 
      model of carbon tetrachloride (CCl4)-induced hepatic fibrosis, the study intended 
      to investigate the hepatoprotective effects of GSPB2 and to determine the 
      possible pathway by which GSPB2 exerts its activities. Design * Thirty-six male, 
      Sprague-Dawley rats were used in the study. Rats in a model (CCl4 only) group and 
      the GSPB2 group were given CCl4 to induce hepatic fibrosis. Simultaneously, 
      animals in the GSPB2 group were treated with GSPB2 by intragastric administration 
      for 12 wk. In addition, the rat's Kupffer cells were cultured with CCl4 and 
      GSPB2. SETTING: The study took place at the central laboratory of Qilu Hospital 
      at Shandong University in Jinan, China. OUTCOME MEASURES: The following 
      parameters were investigated: (1) hepatic function; (2) the liver fibrosis 
      index-serum hyaluronic acid (HA), laminin (LN), type 3 procollagen (PC-3), 
      collagen 4, and hepatic hydroxyproline; (3) the expression in the liver of 
      transforming growth factor beta-1 (TGF-beta1); (4) inflammatory cytokines in the liver 
      and cell culture medium-tumor necrosis factor alpha (TNF-alpha), interleukin (IL) 1-beta 
      (IL-1beta), IL-6, and IL-17; (5) oxidative stress markers in the liver and cell 
      culture medium-malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), total 
      superoxide dismutase (T-SOD), and total antioxidant capacity (T-AOC); and (6) 
      levels of angiotensin 2 (Ang 2) in the liver. RESULTS: The CCl4 induced (1) 
      significant hepatic-function damage; (2) elevated levels of the measures of the 
      liver fibrosis index, TGF-beta1, inflammatory cytokines, MDA, and 8-OHdG; (3) a 
      reduction in the activities of T-SOD and T-AOC; and (4) no effect on the level of 
      expression of hepatic Ang 2. GSPB2 treatment partially reversed the changes 
      induced by CCl4. The cell culture also showed that CCl4 elevated the levels of 
      inflammatory cytokines and MDA in the Kupffer cell culture medium, whereas it 
      reduced the activities of T-SOD and T-AOC in the medium. GSPB2 treatment 
      partially reversed the changes induced by CCl4. CONCLUSIONS: GSPB2 had 
      hepatoprotective effects on CCl4-induced hepatic fibrosis in Sprague-Dawley rats 
      and inhibited the inflammatory response and oxidative stress in vivo and in 
      vitro.
FAU - Wang, Zhenli
AU  - Wang Z
FAU - Zhang, Zemin
AU  - Zhang Z
FAU - Du, Ning
AU  - Du N
FAU - Wang, Kai
AU  - Wang K
FAU - Li, Lei
AU  - Li L
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Altern Ther Health Med
JT  - Alternative therapies in health and medicine
JID - 9502013
RN  - 0 (Antioxidants)
RN  - 0 (Biflavonoids)
RN  - 0 (Cytokines)
RN  - 0 (Grape Seed Extract)
RN  - 0 (Proanthocyanidins)
RN  - 29106-49-8 (procyanidin B2)
RN  - 8R1V1STN48 (Catechin)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Animals
MH  - Antioxidants/chemistry/*pharmacology
MH  - Biflavonoids/chemistry/*pharmacology
MH  - Carbon Tetrachloride/toxicity
MH  - Catechin/chemistry/*pharmacology
MH  - Cytokines/metabolism
MH  - Grape Seed Extract/chemistry/pharmacology
MH  - Liver/chemistry/*drug effects
MH  - Liver Cirrhosis/chemically induced/*drug therapy/metabolism
MH  - Male
MH  - Oxidative Stress/drug effects
MH  - Proanthocyanidins/chemistry/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Seeds/chemistry
MH  - Vitis/*chemistry
EDAT- 2015/08/27 06:00
MHDA- 2015/10/02 06:00
CRDT- 2015/08/27 06:00
PHST- 2015/08/27 06:00 [entrez]
PHST- 2015/08/27 06:00 [pubmed]
PHST- 2015/10/02 06:00 [medline]
PST - ppublish
SO  - Altern Ther Health Med. 2015;21 Suppl 2:12-21.