PMID- 26308792
OWN - NLM
STAT- MEDLINE
DCOM- 20160808
LR  - 20151007
IS  - 1945-0257 (Electronic)
IS  - 1945-0257 (Linking)
VI  - 19
IP  - 10
DP  - 2015 Oct
TI  - Qualitative and quantitative evaluation of the BCR-ABL fusion gene in chronic 
      myelogenous leukemia by flourescence in situ hybridization and molecular genetic 
      methods.
PG  - 584-8
LID - 10.1089/gtmb.2015.0056 [doi]
AB  - AIMS: Fluorescence in situ hybridization (FISH) and quantitative real-time 
      polymerase chain reaction (QRT-PCR) were used to diagnose or screen for minimal 
      residual disease (MRD) in Philadelphia (Ph) chromosome-positive leukemia. We 
      compared the diagnostic utility of FISH and QRT-PCR at various time points in the 
      course of chronic myelogenous leukemia (CML) and to determine the mean initial 
      values for patients whose QRT-PCR results were not known at the time of 
      diagnosis. RESULTS: We analyzed 135 results for 78 CML patients tested by FISH 
      and QRT-PCR for the Ph chromosomal translocation. All newly diagnosed cases were 
      positive by both methods. On follow-up following treatment, 1 case was FISH 
      positive and QRT-PCR negative; 61 cases were FISH negative and QRT-PCR positive. 
      Overall concordance was 54.1%. There was good concordance between QRT-PCR results 
      and cytogenetic response categories. CONCLUSIONS: We confirmed that QRT-PCR 
      allows precise measurement of low levels of BCR-ABL transcripts and can serve as 
      a sensitive indicator of MRD. We also demonstrated 100% correlation between 
      QRT-PCR and FISH in newly diagnosed CML.
FAU - Ozemri Sag, Sebnem
AU  - Ozemri Sag S
AD  - 1 Department of Medical Genetics, Faculty of Medicine, Uludag University , Bursa, 
      Turkey .
FAU - Yakut, Tahsin
AU  - Yakut T
AD  - 1 Department of Medical Genetics, Faculty of Medicine, Uludag University , Bursa, 
      Turkey .
FAU - Gorukmez, Ozlem
AU  - Gorukmez O
AD  - 2 Department of Medical Genetics, Sevket Yilmaz Education and Research Hospital , 
      Bursa, Turkey .
FAU - Gorukmez, Orhan
AU  - Gorukmez O
AD  - 2 Department of Medical Genetics, Sevket Yilmaz Education and Research Hospital , 
      Bursa, Turkey .
FAU - Ture, Mehmet
AU  - Ture M
AD  - 1 Department of Medical Genetics, Faculty of Medicine, Uludag University , Bursa, 
      Turkey .
FAU - Karkucak, Mutlu
AU  - Karkucak M
AD  - 3 Department of Medical Genetics, Sakarya Education and Research Hospital , 
      Sakarya, Turkey .
FAU - Gulten, Tuna
AU  - Gulten T
AD  - 1 Department of Medical Genetics, Faculty of Medicine, Uludag University , Bursa, 
      Turkey .
FAU - Ali, Ridvan
AU  - Ali R
AD  - 4 Division of Hematology, Department of Internal Medicine, Faculty of Medicine, 
      Uludag University , Bursa, Turkey .
LA  - eng
PT  - Journal Article
DEP - 20150826
PL  - United States
TA  - Genet Test Mol Biomarkers
JT  - Genetic testing and molecular biomarkers
JID - 101494210
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Female
MH  - Fusion Proteins, bcr-abl/blood/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/blood/*genetics
MH  - Male
MH  - Middle Aged
MH  - Neoplasm, Residual
MH  - Real-Time Polymerase Chain Reaction/methods
MH  - Reproducibility of Results
EDAT- 2015/08/27 06:00
MHDA- 2016/08/09 06:00
CRDT- 2015/08/27 06:00
PHST- 2015/08/27 06:00 [entrez]
PHST- 2015/08/27 06:00 [pubmed]
PHST- 2016/08/09 06:00 [medline]
AID - 10.1089/gtmb.2015.0056 [doi]
PST - ppublish
SO  - Genet Test Mol Biomarkers. 2015 Oct;19(10):584-8. doi: 10.1089/gtmb.2015.0056. 
      Epub 2015 Aug 26.