PMID- 26309577
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150828
LR  - 20200930
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 8
IP  - 6
DP  - 2015
TI  - Mitochondrial and cytoskeletal alterations are involved in the pathogenesis of 
      hydronephrosis in ICR/Mlac-hydro mice.
PG  - 9192-204
AB  - The pathogenesis of congenital hydronephrosis in laboratory animals has been 
      studied for many years, yet little is known about the underlying mechanism of 
      this disease. In this study, we investigated a MS-based comparative proteomics 
      approach to characterize the differently expressed proteins between kidney tissue 
      samples of ICR/Mlac-hydro and wild-type mice. Interestingly, proteomic results 
      exhibited several mitochondrial protein alterations especially the up-regulation 
      of 60 kDa heat shock protein (Hsp60), stress-70 protein (GRP75) dysfunction, and 
      down-regulation of voltage-dependent anion-selective channel protein 1 (VDAC-1). 
      The results demonstrated that mitochondrial alteration may lead to inadequate 
      energy-supply to maintain normal water reabsorption from the renal tubule, 
      causing hydronephrosis. Moreover, the alteration of cytoskeleton proteins in the 
      renal tubule, in particular the up-regulation of tubulin beta-4B chain (Tb4B) and 
      N-myc downstream-regulated gene 1 protein (Ndr-1) may also be related due to 
      their fundamental roles in maintaining cell morphology and tissue stability. In 
      addition, cytoskeletal alterations may consequence to the reduction of 
      glyceraldehydes-3-phosphate dehydrogenase (GAPDH), cytoplasmic enzyme, which 
      modulates the capacity of structural proteins. Our findings highlight a number of 
      target proteins that may play a crucial role in congenital hydronephrosis and 
      emphasize that the disorder of mitochondria and cytoskeleton proteins may be 
      involved.
FAU - Isarangkul, Duangnate
AU  - Isarangkul D
AD  - Department of Microbiology, Faculty of Science, Mahidol University 272, Rama VI 
      Road, Ratchathewi, Bangkok 10400, Thailand.
FAU - Wiyakrutta, Suthep
AU  - Wiyakrutta S
AD  - Department of Microbiology, Faculty of Science, Mahidol University 272, Rama VI 
      Road, Ratchathewi, Bangkok 10400, Thailand.
FAU - Kengkoom, Kanchana
AU  - Kengkoom K
AD  - Office of Academic Services, National Laboratory Animal Center, Mahidol 
      University 999, Phutthamonthon 4 Road, Salaya, Phutthamonthon, Nakhon Pathom 
      73170, Thailand.
FAU - Reamtong, Onrapak
AU  - Reamtong O
AD  - Department of Molecular Tropical Medicine and Genetic, Faculty of Tropical 
      Medicine, Mahidol University 420/6, Ratchawithi Road, Ratchathewi, Bangkok 10400, 
      Thailand.
FAU - Ampawong, Sumate
AU  - Ampawong S
AD  - Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol 
      University 420/6, Ratchawithi Road, Ratchathewi, Bangkok 10400, Thailand.
LA  - eng
PT  - Journal Article
DEP - 20150615
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4538032
OTO - NOTNLM
OT  - Cytoskeleton
OT  - ICR/Mlac-hydro mice
OT  - hydronephrosis
OT  - mitochondria
OT  - pathogenesis
OT  - proteomic
EDAT- 2015/08/27 06:00
MHDA- 2015/08/27 06:01
PMCR- 2015/06/15
CRDT- 2015/08/27 06:00
PHST- 2015/04/08 00:00 [received]
PHST- 2015/06/07 00:00 [accepted]
PHST- 2015/08/27 06:00 [entrez]
PHST- 2015/08/27 06:00 [pubmed]
PHST- 2015/08/27 06:01 [medline]
PHST- 2015/06/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2015 Jun 15;8(6):9192-204. eCollection 2015.