PMID- 26311539
OWN - NLM
STAT- MEDLINE
DCOM- 20160623
LR  - 20181113
IS  - 1435-232X (Electronic)
IS  - 1434-5161 (Print)
IS  - 1434-5161 (Linking)
VI  - 60
IP  - 11
DP  - 2015 Nov
TI  - The impact of next-generation sequencing technologies on HLA research.
PG  - 665-73
LID - 10.1038/jhg.2015.102 [doi]
AB  - In the past decade, the development of next-generation sequencing (NGS) has paved 
      the way for whole-genome analysis in individuals. Research on the human leukocyte 
      antigen (HLA), an extensively studied molecule involved in immunity, has 
      benefitted from NGS technologies. The HLA region, a 3.6-Mb segment of the human 
      genome at 6p21, has been associated with more than 100 different diseases, 
      primarily autoimmune diseases. Recently, the HLA region has received much 
      attention because severe adverse effects of various drugs are associated with 
      particular HLA alleles. Owing to the complex nature of the HLA genes, classical 
      direct sequencing methods cannot comprehensively elucidate the genomic makeup of 
      HLA genes. Thus far, several high-throughput HLA-typing methods using NGS have 
      been developed. In HLA research, NGS facilitates complete HLA sequencing and is 
      expected to improve our understanding of the mechanisms through which HLA genes 
      are modulated, including transcription, regulation of gene expression and 
      epigenetics. Most importantly, NGS may also permit the analysis of HLA-omics. In 
      this review, we summarize the impact of NGS on HLA research, with a focus on the 
      potential for clinical applications.
FAU - Hosomichi, Kazuyoshi
AU  - Hosomichi K
AD  - Department of Bioinformatics and Genomics, Graduate School of Medical Sciences, 
      Kanazawa University, Ishikawa, Japan.
FAU - Shiina, Takashi
AU  - Shiina T
AD  - Department of Molecular Life Science, Division of Basic Medical Science and 
      Molecular Medicine, Tokai University School of Medicine, Kanagawa, Japan.
FAU - Tajima, Atsushi
AU  - Tajima A
AUID- ORCID: 0000-0001-6808-5491
AD  - Department of Bioinformatics and Genomics, Graduate School of Medical Sciences, 
      Kanazawa University, Ishikawa, Japan.
FAU - Inoue, Ituro
AU  - Inoue I
AD  - Division of Human Genetics, National Institute of Genetics, Shizuoka, Japan.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20150827
PL  - England
TA  - J Hum Genet
JT  - Journal of human genetics
JID - 9808008
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Alleles
MH  - Genome, Human
MH  - Genome-Wide Association Study
MH  - HLA Antigens/*genetics
MH  - High-Throughput Nucleotide Sequencing/*methods
MH  - Histocompatibility Testing/methods
MH  - Humans
MH  - Sequence Analysis, DNA/methods
MH  - Software
PMC - PMC4660052
EDAT- 2015/08/28 06:00
MHDA- 2016/06/24 06:00
PMCR- 2015/11/26
CRDT- 2015/08/28 06:00
PHST- 2015/05/18 00:00 [received]
PHST- 2015/06/10 00:00 [revised]
PHST- 2015/07/21 00:00 [accepted]
PHST- 2015/08/28 06:00 [entrez]
PHST- 2015/08/28 06:00 [pubmed]
PHST- 2016/06/24 06:00 [medline]
PHST- 2015/11/26 00:00 [pmc-release]
AID - jhg2015102 [pii]
AID - 10.1038/jhg.2015.102 [doi]
PST - ppublish
SO  - J Hum Genet. 2015 Nov;60(11):665-73. doi: 10.1038/jhg.2015.102. Epub 2015 Aug 27.