PMID- 26314340
OWN - NLM
STAT- MEDLINE
DCOM- 20160818
LR  - 20211119
IS  - 1205-7541 (Electronic)
IS  - 0008-4212 (Linking)
VI  - 93
IP  - 11
DP  - 2015 Nov
TI  - The potential anti-proliferative effect of beta-sitosterol on human mast cell line-1 
      cells.
PG  - 979-83
LID - 10.1139/cjpp-2015-0166 [doi]
AB  - Thymic stromal lymphopoietin (TSLP) was reported to induce mast cell 
      proliferation and aggravate allergic reactions through activation of mouse double 
      minute 2 (MDM2). We aimed to ascertain that beta-sitosterol (SI), which is one of 
      the several phytosterols found mostly in foods, would regulate TSLP-induced mast 
      cell proliferation. The results showed that SI significantly decreased the 
      proliferation of human mast cell line (HMC-1) cells promoted by TSLP. SI 
      significantly decreased the mRNA expression of Ki-67 in the TSLP-treated HMC-1 
      cells. SI significantly suppressed the production and mRNA expression of 
      interleukin-13 in the TSLP-treated HMC-1 cells. Furthermore, SI downregulated the 
      expression of MDM2 and phosphorylation of STAT6, whereas it upregulated the 
      expression of p53, activation of caspase-3, and cleavage of poly ADP-ribose 
      polymerase in the TSLP-treated HMC-1 cells. Results of this study suggest that SI 
      may be a potential therapeutic agent for mast cell-mediated allergic diseases.
FAU - Han, Na-Ra
AU  - Han NR
AD  - a Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 
      26, Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
FAU - Kim, Hyung-Min
AU  - Kim HM
AD  - a Department of Pharmacology, College of Korean Medicine, Kyung Hee University, 
      26, Kyungheedae-ro, Dongdaemun-gu, Seoul 130-701, Republic of Korea.
FAU - Jeong, Hyun-Ja
AU  - Jeong HJ
AD  - b Department of Food Technology and Inflammatory Disease Research Center, Hoseo 
      University, 20, Hoseo-ro 79beon-gil, Baebang-eup, Asan 336-795, Republic of 
      Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150508
PL  - Canada
TA  - Can J Physiol Pharmacol
JT  - Canadian journal of physiology and pharmacology
JID - 0372712
RN  - 0 (IL13 protein, human)
RN  - 0 (Interleukin-13)
RN  - 0 (Sitosterols)
RN  - 5LI01C78DD (gamma-sitosterol)
SB  - IM
MH  - Cell Line
MH  - Cell Proliferation/*drug effects/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Interleukin-13/antagonists & inhibitors/metabolism
MH  - Mast Cells/*drug effects/*metabolism
MH  - Sitosterols/*pharmacology
OTO - NOTNLM
OT  - lymphopoietine stromale thymique
OT  - mast cells
OT  - mastocytes
OT  - mouse double minute 2
OT  - proliferation
OT  - proliferation
OT  - thymic stromal lymphopoietin
OT  - beta-sitosterol
OT  - beta-sitosterol
EDAT- 2015/09/01 06:00
MHDA- 2016/08/19 06:00
CRDT- 2015/08/29 06:00
PHST- 2015/08/29 06:00 [entrez]
PHST- 2015/09/01 06:00 [pubmed]
PHST- 2016/08/19 06:00 [medline]
AID - 10.1139/cjpp-2015-0166 [doi]
PST - ppublish
SO  - Can J Physiol Pharmacol. 2015 Nov;93(11):979-83. doi: 10.1139/cjpp-2015-0166. 
      Epub 2015 May 8.