PMID- 26315473
OWN - NLM
STAT- MEDLINE
DCOM- 20160815
LR  - 20221207
IS  - 1432-0711 (Electronic)
IS  - 0932-0067 (Linking)
VI  - 293
IP  - 4
DP  - 2016 Apr
TI  - Simultaneous detection of human papillomavirus integration and c-MYC gene 
      amplification in cervical lesions: an emerging marker for the risk to 
      progression.
PG  - 857-63
LID - 10.1007/s00404-015-3870-3 [doi]
AB  - PURPOSE: The persistence of high-risk oncogenic human papillomavirus (HR-HPV) 
      infection and its integration into the host genome are key steps in the induction 
      of malignant alterations. c-MYC chromosome region is a frequent localization for 
      HPV insertion that has been observed in chromosome band 8q24 by fluorescence in 
      situ hybridization (FISH). We report the HPV viral integration and amplification 
      patterns of the c-MYC gene in cytological smears with FISH as a potential 
      biomarker for the progression of squamous intraepithelial lesions (SIL). METHODS: 
      HPV detection and genotyping by polymerase chain reaction (PCR) and FISH analysis 
      by "Vysis Cervical FISH Probe" kit (ABBOTT Molecular Inc.) were performed in 37 
      cervical samples including 8 NILM, 7 ASC-US, 7 LSIL, 3 ASC-H, 7 HSIL and 5 SCC. 
      RESULTS: The results show concordance between FISH and PCR techniques for HPV 
      detection. The majority of the samples contained HR-HPV, the majority being -16 
      and -18 genotypes. HPV integration as determined by FISH was most frequent in 
      high-risk lesions. The c-MYC gene amplification was found only in HPV-positive 
      samples and was detected primarily in high-risk lesions and in cells with an 
      integrated form of HPV. CONCLUSIONS: HPV integration and c-MYC gene amplification 
      detected by FISH could be an important biomarker for use in clinical practice to 
      determine SIL with a risk of progression.
FAU - Gimenes, Fabricia
AU  - Gimenes F
AD  - Laboratory of Clinical Cytology and Semen Analysis, Department of Clinical 
      Analysis and Biomedicine, State University of Maringa, Maringa, PR, 87020900, 
      Brazil.
FAU - Souza, Raquel Pantarotto
AU  - Souza RP
AD  - Laboratory of Clinical Cytology and Semen Analysis, Department of Clinical 
      Analysis and Biomedicine, State University of Maringa, Maringa, PR, 87020900, 
      Brazil.
FAU - de Abreu, Andre Luelsdorf Pimenta
AU  - de Abreu AL
AD  - Laboratory of Clinical Cytology and Semen Analysis, Department of Clinical 
      Analysis and Biomedicine, State University of Maringa, Maringa, PR, 87020900, 
      Brazil.
FAU - Pereira, Monalisa Wolski
AU  - Pereira MW
AD  - Laboratory of Clinical Cytology and Semen Analysis, Department of Clinical 
      Analysis and Biomedicine, State University of Maringa, Maringa, PR, 87020900, 
      Brazil.
FAU - Consolaro, Marcia Edilaine Lopes
AU  - Consolaro ME
AD  - Laboratory of Clinical Cytology and Semen Analysis, Department of Clinical 
      Analysis and Biomedicine, State University of Maringa, Maringa, PR, 87020900, 
      Brazil.
FAU - da Silva, Vania Ramos Sela
AU  - da Silva VR
AD  - Laboratory of Clinical Cytology and Semen Analysis, Department of Clinical 
      Analysis and Biomedicine, State University of Maringa, Maringa, PR, 87020900, 
      Brazil. vaniasela@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150828
PL  - Germany
TA  - Arch Gynecol Obstet
JT  - Archives of gynecology and obstetrics
JID - 8710213
SB  - IM
MH  - Adult
MH  - Disease Progression
MH  - Female
MH  - *Gene Amplification
MH  - Genes, myc/*genetics
MH  - Genotype
MH  - Human papillomavirus 16/genetics
MH  - Human papillomavirus 18/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Middle Aged
MH  - Papillomaviridae/*genetics
MH  - Papillomavirus Infections/*diagnosis/genetics/virology
MH  - Polymerase Chain Reaction/methods
MH  - Squamous Intraepithelial Lesions of the Cervix/diagnosis/*genetics/virology
MH  - Uterine Cervical Dysplasia/diagnosis/genetics/virology
MH  - Uterine Cervical Neoplasms/diagnosis/*genetics/virology
OTO - NOTNLM
OT  - Biomarkers
OT  - Cervical cancer
OT  - Human papillomavirus
OT  - Integration
OT  - c-MYC
EDAT- 2015/09/01 06:00
MHDA- 2016/08/16 06:00
CRDT- 2015/08/29 06:00
PHST- 2015/06/16 00:00 [received]
PHST- 2015/08/18 00:00 [accepted]
PHST- 2015/08/29 06:00 [entrez]
PHST- 2015/09/01 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
AID - 10.1007/s00404-015-3870-3 [pii]
AID - 10.1007/s00404-015-3870-3 [doi]
PST - ppublish
SO  - Arch Gynecol Obstet. 2016 Apr;293(4):857-63. doi: 10.1007/s00404-015-3870-3. Epub 
      2015 Aug 28.