PMID- 26317310
OWN - NLM
STAT- MEDLINE
DCOM- 20170822
LR  - 20180313
IS  - 1533-4058 (Electronic)
IS  - 1533-4058 (Linking)
VI  - 24
IP  - 7
DP  - 2016 Aug
TI  - HER2 Gene Amplification by Fluorescence In Situ Hybridization (FISH) Compared 
      With Immunohistochemistry (IHC) in 122 Equivocal Gastric Cancer Cases.
PG  - 459-64
LID - 10.1097/PAI.0000000000000219 [doi]
AB  - OBJECTIVE: To determine the frequency of HER2/neu amplification and the 
      relationship between HER2/neu expression and clinicopathologic features in 
      gastric cancers scored immunohistochemistry (IHC) 2+. METHODS: A total of 122 
      gastric cancer cases scored IHC 2+ were retrospectively analyzed for HER2 
      amplification by fluorescence in situ hybridization (FISH). The correlation 
      between HER2/neu expression and clinicopathologic features was analyzed. RESULTS: 
      HER2/neu gene was amplified in 17 out of the 122 gastric cancer samples. The 
      concordance rate between IHC and FISH was 13.9%. HER2/neu status was correlated 
      with the age of patients (P<0.05). Polysomy of CEP17 was demonstrated in 46 
      cases, 11 of which (23.91%) were amplified for HER2/neu. Within the subgroups, a 
      correlation between CEP17 polysomy and the depth of invasion was observed 
      (P<0.05). CONCLUSIONS: The results highlight the necessity of FISH test for 
      further categorization when gastric cancer cases are scored 2+ by IHC. The value 
      of HER2/neu for a potential role as a negative prognostic factor in the equivocal 
      gastric cancer cases is limited.
FAU - Liu, Xiaoyan
AU  - Liu X
AD  - Department of Pathology, The First Affiliated Hospital, College of Medicine, 
      Zhejiang University, Hangzhou, Zhejiang, China.
FAU - Wang, Xiaoling
AU  - Wang X
FAU - Wang, Bo
AU  - Wang B
FAU - Ren, Guoping
AU  - Ren G
FAU - Ding, Wei
AU  - Ding W
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Appl Immunohistochem Mol Morphol
JT  - Applied immunohistochemistry & molecular morphology : AIMM
JID - 100888796
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*diagnosis/pathology
MH  - Female
MH  - Gene Amplification
MH  - Gene Frequency
MH  - Humans
MH  - *Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Receptor, ErbB-2/*genetics
MH  - Retrospective Studies
MH  - Stomach Neoplasms/*diagnosis/pathology
MH  - Tumor Burden
EDAT- 2015/09/01 06:00
MHDA- 2017/08/23 06:00
CRDT- 2015/08/29 06:00
PHST- 2015/08/29 06:00 [entrez]
PHST- 2015/09/01 06:00 [pubmed]
PHST- 2017/08/23 06:00 [medline]
AID - 10.1097/PAI.0000000000000219 [doi]
PST - ppublish
SO  - Appl Immunohistochem Mol Morphol. 2016 Aug;24(7):459-64. doi: 
      10.1097/PAI.0000000000000219.