PMID- 26318305
OWN - NLM
STAT- MEDLINE
DCOM- 20170130
LR  - 20181113
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 37
IP  - 2
DP  - 2016 Feb
TI  - Upregulation of miR-129-5p affects laryngeal cancer cell proliferation, 
      invasiveness, and migration by affecting STAT3 expression.
PG  - 1789-96
LID - 10.1007/s13277-015-3969-y [doi]
AB  - microRNA-129-5p may have a relationship with laryngeal squamous cell carcinoma 
      (LSCC), but the expression and function of miRNA-129-5p are not fully understood. 
      This study was performed to investigate the expression and function of 
      miRNA-129-5p in LSCC and its effects on STAT3. HBE and Hep-2 cells were cultured 
      and analyzed by western blotting and real-time PCR. miR-129-5p expression was 
      detected by real-time PCR. Hep-2 cells were transfected with a miR-129-5p 
      expression vector. Cell proliferation, invasion, migration, cell cycle, and 
      apoptosis assays were used to determine the role of miRNA-129-5p in the growth, 
      invasion, and migration of LSCC cells and to determine the importance of STAT3 in 
      these effects. STAT3 mRNA expression in Hep-2 cells was significantly higher than 
      that in HBE cells (P < 0.05). miR-129-5p expression detected by real-time PCR 
      showed that it was expressed at a lower level in Hep-2 cells than in HBE cells 
      (P < 0.05). Compared with the control cells, the transfected cells showed lower 
      STAT3 mRNA expression. For up to 7 days in culture, the transfected cells showed 
      lower proliferation than the control cells (P < 0.05). After 24 h in culture, the 
      apoptosis rate in miR-129-5p-transfected cells was 3.48 +/- 0.38 %, while the rate 
      in control cells was 0.92 +/- 0.09 % (P = 0.0028), but the statistical significance 
      was lost after 72 h in culture (P = 0.3180). The invasion and migration of the 
      cells were inhibited after 24 and 72 h in culture when the miR-129-5p expression 
      in Hep-2 cells was upregulated (P = 0.0037 and 0.00383, respectively), while 
      there was no statistically significant difference at 48 h (P = 0.0712). STAT3 
      expression could be suppressed by the upregulation of miR-129-5p expression. Both 
      the proliferation and migration of tumor cells were suppressed when the level of 
      STAT3 expression was decreased. The apoptosis rate of tumor cells was also 
      increased. Based on these data, we suggest that miR-129-5p may directly inhibit 
      STAT3 expression and play an important role in the development of LSCC.
FAU - Shen, Na
AU  - Shen N
AD  - Department of Otolaryngology, Zhongshan Hospital, Fudan University, 180 Fenglin 
      Road, Shanghai, 200032, China.
FAU - Huang, Xinsheng
AU  - Huang X
AD  - Department of Otolaryngology, Zhongshan Hospital, Fudan University, 180 Fenglin 
      Road, Shanghai, 200032, China.
FAU - Li, Jun
AU  - Li J
AD  - Department of Otolaryngology, Qingpu Branch of Zhongshan Hospital, Fudan 
      University, 1158 East Gongyuan Road, Qinpu District, Shanghai, 201700, China. 
      lijun_ent@163.com.
LA  - eng
PT  - Journal Article
DEP - 20150829
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn129 microRNA, human)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
SB  - IM
MH  - Apoptosis/genetics
MH  - Carcinoma, Squamous Cell/genetics/pathology
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/*genetics
MH  - Cell Proliferation/*genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness/*genetics/pathology
MH  - RNA, Messenger/genetics
MH  - STAT3 Transcription Factor/*genetics
MH  - Transfection/methods
MH  - Up-Regulation/*genetics
OTO - NOTNLM
OT  - Apoptosis
OT  - Invasion
OT  - Laryngeal squamous cells carcinoma
OT  - STAT3
OT  - miRNA-129-5p
EDAT- 2015/09/01 06:00
MHDA- 2017/01/31 06:00
CRDT- 2015/08/31 06:00
PHST- 2015/07/10 00:00 [received]
PHST- 2015/08/21 00:00 [accepted]
PHST- 2015/08/31 06:00 [entrez]
PHST- 2015/09/01 06:00 [pubmed]
PHST- 2017/01/31 06:00 [medline]
AID - 10.1007/s13277-015-3969-y [pii]
AID - 10.1007/s13277-015-3969-y [doi]
PST - ppublish
SO  - Tumour Biol. 2016 Feb;37(2):1789-96. doi: 10.1007/s13277-015-3969-y. Epub 2015 
      Aug 29.