PMID- 26318571
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20211203
IS  - 1878-0180 (Electronic)
IS  - 1878-0180 (Linking)
VI  - 53
DP  - 2016 Jan
TI  - Adhesive strength of bone-implant interfaces and in-vivo degradation of PHB 
      composites for load-bearing applications.
PG  - 104-118
LID - S1751-6161(15)00275-1 [pii]
LID - 10.1016/j.jmbbm.2015.08.004 [doi]
AB  - Aim of this study was to evaluate the response of bone to novel biodegradable 
      polymeric composite implants in the femora of growing rats. Longitudinal 
      observation of bone reaction at the implant site (BV/TV) as well as resorption of 
      the implanted pins were monitored using in vivo micro-focus computed tomography 
      (microCT). After 12, 24 and 36 weeks femora containing the implants were explanted, 
      scanned with high resolution ex vivo microCT, and the surface roughness of the 
      implants was measured to conclude on the ingrowth capability for bone tissue. 
      Scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDX) 
      were used to observe changes on the surface of Polyhydroxybutyrate (PHB) during 
      degradation and cell ingrowth. Four different composites with zirconium dioxide 
      (ZrO2) and Herafill((R)) were compared. After 36 weeks in vivo, none of the 
      implants did show significant degradation. The PHB composite with ZrO2 and a high 
      percentage (30%) of Herafill(R) as well as the Mg-alloy WZ21 showed the highest 
      values of bone accumulation (increased BV/TV) around the implant. The lowest 
      value was measured in PHB with 3% ZrO2 containing no Herafill(R). Roughness 
      measurements as well as EDX and SEM imaging could not reveal any changes on the 
      PHB composites׳ surfaces. Biomechanical parameters, such as the adhesion strength 
      between bone and implant were determined by measuring the shear strength as well 
      as push-out energy of the bone-implant interface. The results showed that 
      improvement of these mechanical properties of the studied PHBs P3Z, P3Z10H and 
      P3Z30H is necessary in order to obtain appropriate load-bearing material. The 
      moduli of elasticity, tensile strength and strain properties of the PHB 
      composites are close to that of bone and thus promising. Compared to clinically 
      used PLGA, PGA and PLA materials, their additional benefit is an unchanged local 
      pH value during degradation, which makes them well tolerated by cells and immune 
      system. They might be used successfully for personalized 3D printed implants or 
      as coatings of rapidly dissolving implants.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Meischel, M
AU  - Meischel M
AD  - University of Natural Resources and Life Sciences, Institute of Physics and 
      Materials Science, Peter-Jordan-Strasse 82, 1190 Vienna, Austria.
FAU - Eichler, J
AU  - Eichler J
AD  - Medical University of Graz, Department of Orthopedic Surgery, Auenbruggerplatz 5, 
      8036 Graz, Austria.
FAU - Martinelli, E
AU  - Martinelli E
AD  - Medical University of Graz, Department of Orthopedic Surgery, Auenbruggerplatz 5, 
      8036 Graz, Austria.
FAU - Karr, U
AU  - Karr U
AD  - University of Natural Resources and Life Sciences, Institute of Physics and 
      Materials Science, Peter-Jordan-Strasse 82, 1190 Vienna, Austria.
FAU - Weigel, J
AU  - Weigel J
AD  - Medical University of Graz, Department of Orthopedic Surgery, Auenbruggerplatz 5, 
      8036 Graz, Austria.
FAU - Schmoller, G
AU  - Schmoller G
AD  - Medical University of Graz, Department of Orthopedic Surgery, Auenbruggerplatz 5, 
      8036 Graz, Austria.
FAU - Tschegg, E K
AU  - Tschegg EK
AD  - Vienna Technical University, Faculty of Physics, Institute of Solid State 
      Physics, Karlsplatz 13, 1040 Wien, Austria.
FAU - Fischerauer, S
AU  - Fischerauer S
AD  - Medical University of Graz, Department of Orthopedic Surgery, Auenbruggerplatz 5, 
      8036 Graz, Austria.
FAU - Weinberg, A M
AU  - Weinberg AM
AD  - Medical University of Graz, Department of Orthopedic Surgery, Auenbruggerplatz 5, 
      8036 Graz, Austria.
FAU - Stanzl-Tschegg, S E
AU  - Stanzl-Tschegg SE
AD  - University of Natural Resources and Life Sciences, Institute of Physics and 
      Materials Science, Peter-Jordan-Strasse 82, 1190 Vienna, Austria. Electronic 
      address: stefanie.tschegg@boku.ac.at.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150812
PL  - Netherlands
TA  - J Mech Behav Biomed Mater
JT  - Journal of the mechanical behavior of biomedical materials
JID - 101322406
RN  - 0 (Hydroxybutyrates)
RN  - 0 (Phb protein, rat)
RN  - 0 (Polyesters)
RN  - 0 (Prohibitins)
RN  - 26063-00-3 (poly-beta-hydroxybutyrate)
SB  - IM
MH  - Adhesiveness
MH  - Animals
MH  - *Bone-Implant Interface
MH  - Drug Stability
MH  - Femur/diagnostic imaging/metabolism
MH  - Hydroxybutyrates/*chemistry/*metabolism
MH  - Male
MH  - Materials Testing
MH  - Polyesters/*chemistry/*metabolism
MH  - Prohibitins
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Shear Strength
MH  - Weight-Bearing
MH  - X-Ray Microtomography
OTO - NOTNLM
OT  - Bone-implant interface
OT  - Bone-cell ingrowth
OT  - In vivo degradation
OT  - Polymeric bone implants
OT  - Surface roughness
OT  - microCT
EDAT- 2015/09/01 06:00
MHDA- 2016/09/16 06:00
CRDT- 2015/08/31 06:00
PHST- 2015/05/12 00:00 [received]
PHST- 2015/07/28 00:00 [revised]
PHST- 2015/08/04 00:00 [accepted]
PHST- 2015/08/31 06:00 [entrez]
PHST- 2015/09/01 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
AID - S1751-6161(15)00275-1 [pii]
AID - 10.1016/j.jmbbm.2015.08.004 [doi]
PST - ppublish
SO  - J Mech Behav Biomed Mater. 2016 Jan;53:104-118. doi: 10.1016/j.jmbbm.2015.08.004. 
      Epub 2015 Aug 12.