PMID- 26319788
OWN - NLM
STAT- MEDLINE
DCOM- 20160817
LR  - 20191210
IS  - 1432-2099 (Electronic)
IS  - 0301-634X (Linking)
VI  - 54
IP  - 4
DP  - 2015 Nov
TI  - Verification by the FISH translocation assay of historic doses to Mayak workers 
      from external gamma radiation.
PG  - 445-51
LID - 10.1007/s00411-015-0614-5 [doi]
AB  - The aim of this study was to apply the fluorescence in situ hybridization (FISH) 
      translocation assay in combination with chromosome painting of peripheral blood 
      lymphocytes for retrospective biological dosimetry of Mayak nuclear power plant 
      workers exposed chronically to external gamma radiation. These data were compared 
      with physical dose estimates based on monitoring with badge dosimeters throughout 
      each person's working life. Chromosome translocation yields for 94 workers of the 
      Mayak production association were measured in three laboratories: Southern Urals 
      Biophysics Institute, Leiden University Medical Center and the former Health 
      Protection Agency of the UK (hereinafter Public Health England). The results of 
      the study demonstrated that the FISH-based translocation assay in workers with 
      prolonged (chronic) occupational gamma-ray exposure was a reliable biological 
      dosimeter even many years after radiation exposure. Cytogenetic estimates of red 
      bone marrow doses from external gamma rays were reasonably consistent with dose 
      measurements based on film badge readings successfully validated in dosimetry 
      system "Doses-2005" by FISH, within the bounds of the associated uncertainties.
FAU - Sotnik, Natalia V
AU  - Sotnik NV
AD  - Southern Urals Biophysics Institute (SUBI), 19 Ozyorskoe Shosse, Ozyorsk, 
      Chelyabinsk Region, 456780, Russian Federation. sotnik@subi.su.
FAU - Azizova, Tamara V
AU  - Azizova TV
AD  - Southern Urals Biophysics Institute (SUBI), 19 Ozyorskoe Shosse, Ozyorsk, 
      Chelyabinsk Region, 456780, Russian Federation.
FAU - Darroudi, Firouz
AU  - Darroudi F
AD  - Department of Toxicogenetics, Leiden University Medical Center, Leiden, The 
      Netherlands.
AD  - Department of Health Science, Centre for Human Safety and Environmental Research, 
      College of North Atlantic, Doha, Qatar.
FAU - Ainsbury, Elizabeth A
AU  - Ainsbury EA
AD  - Public Health England, Chilton, Oxfordshire, UK.
FAU - Moquet, Jayne E
AU  - Moquet JE
AD  - Public Health England, Chilton, Oxfordshire, UK.
FAU - Fomina, Janna
AU  - Fomina J
AD  - Department of Toxicogenetics, Leiden University Medical Center, Leiden, The 
      Netherlands.
FAU - Lloyd, David C
AU  - Lloyd DC
AD  - Public Health England, Chilton, Oxfordshire, UK.
FAU - Hone, Pat A
AU  - Hone PA
AD  - Public Health England, Chilton, Oxfordshire, UK.
FAU - Edwards, Alan A
AU  - Edwards AA
AD  - Public Health England, Chilton, Oxfordshire, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20150830
PL  - Germany
TA  - Radiat Environ Biophys
JT  - Radiation and environmental biophysics
JID - 0415677
SB  - IM
MH  - Absorption, Radiation
MH  - Aged
MH  - Biological Assay/*methods
MH  - Chromosome Aberrations/*radiation effects
MH  - Chromosome Painting
MH  - Female
MH  - Gamma Rays
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*physiology/radiation effects
MH  - Male
MH  - Occupational Exposure/*analysis
MH  - Radiation Exposure/*analysis
MH  - Radioactive Hazard Release
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic/radiation effects
MH  - Whole-Body Counting
OTO - NOTNLM
OT  - Badge doses
OT  - Dose validation
OT  - External gamma rays
OT  - FISH
OT  - Translocations
EDAT- 2015/09/01 06:00
MHDA- 2016/08/18 06:00
CRDT- 2015/08/31 06:00
PHST- 2014/12/17 00:00 [received]
PHST- 2015/08/23 00:00 [accepted]
PHST- 2015/08/31 06:00 [entrez]
PHST- 2015/09/01 06:00 [pubmed]
PHST- 2016/08/18 06:00 [medline]
AID - 10.1007/s00411-015-0614-5 [pii]
AID - 10.1007/s00411-015-0614-5 [doi]
PST - ppublish
SO  - Radiat Environ Biophys. 2015 Nov;54(4):445-51. doi: 10.1007/s00411-015-0614-5. 
      Epub 2015 Aug 30.