PMID- 26322102
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150831
LR  - 20200930
IS  - 1734-1922 (Print)
IS  - 1896-9151 (Electronic)
IS  - 1734-1922 (Linking)
VI  - 11
IP  - 4
DP  - 2015 Aug 12
TI  - Different methods for inducing adipose-derived stem cells to differentiate into 
      Schwann-like cells.
PG  - 886-92
LID - 10.5114/aoms.2015.53310 [doi]
AB  - INTRODUCTION: The aim of the study was to explore an effective method to induce 
      adipose-derived stem cells (ADSCs) to differentiate into Schwann-like cells in 
      vitro. MATERIAL AND METHODS: Reagents were applied in two different ways (Dezawa 
      inducing method and modified inducing method) in which inducers including 
      beta-mercaptoethanol (beta-ME), all-trans-retinoic acid (ATRA), type I collagenase, 
      forskolin, heregulin, basic fibroblast growth factor (BFGF) and brain-derived 
      neurotrophic factor (BDNF) were used in different ways to induce ADSCs of rats to 
      differentiate into Schwann-like cells. After induction, the cell morphologic 
      characteristics and the cellular immunohistochemical staining positive rate of 
      anti-S100 and anti-GFAP (glial fibrillary acidic protein) antibodies and the gray 
      value of immunocytochemical dye with anti-S100 and anti-GFAP antibodies and cell 
      activity measured by the MTT method were compared with each other to evaluate the 
      induction effects. RESULTS: Both methods can induce differentiation of ADSCs of 
      rats into Schwann-like cells, but the cellular morphology of the modified method 
      was more similar to Schwann cells than that of the Dezawa inducing method, there 
      was a higher cellular immunohistochemical staining positive rate and staining 
      grey value in immunocytochemical dye with anti-S100 and anti-GFAP antibodies, and 
      less damage in the cell activity of the modified inducing method than that of the 
      Dezawa inducing method. CONCLUSIONS: The effect of the modified method to induce 
      ADSCs to differentiate into Schwann-like cells in vitro is superior to that of 
      the Dezawa inducing method.
FAU - Gao, Songtao
AU  - Gao S
AD  - Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Zheng, Yan
AU  - Zheng Y
AD  - Department of Radiology, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Cai, Qiqing
AU  - Cai Q
AD  - Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Wu, Xuejian
AU  - Wu X
AD  - Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Yao, Weitao
AU  - Yao W
AD  - Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, 
      Zhengzhou, China.
FAU - Wang, Jiaqiang
AU  - Wang J
AD  - Department of Orthopedics, The Affiliated Tumor Hospital of Zhengzhou University, 
      Zhengzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20150811
PL  - Poland
TA  - Arch Med Sci
JT  - Archives of medical science : AMS
JID - 101258257
PMC - PMC4548042
OTO - NOTNLM
OT  - MTT
OT  - S100
OT  - Schwann-like cells
OT  - adipose-derived stem cells
OT  - glial fibrillary acidic protein
EDAT- 2015/09/01 06:00
MHDA- 2015/09/01 06:01
PMCR- 2015/08/12
CRDT- 2015/09/01 06:00
PHST- 2013/07/16 00:00 [received]
PHST- 2013/08/12 00:00 [revised]
PHST- 2013/08/26 00:00 [accepted]
PHST- 2015/09/01 06:00 [entrez]
PHST- 2015/09/01 06:00 [pubmed]
PHST- 2015/09/01 06:01 [medline]
PHST- 2015/08/12 00:00 [pmc-release]
AID - 25590 [pii]
AID - 10.5114/aoms.2015.53310 [doi]
PST - ppublish
SO  - Arch Med Sci. 2015 Aug 12;11(4):886-92. doi: 10.5114/aoms.2015.53310. Epub 2015 
      Aug 11.