PMID- 26323359
OWN - NLM
STAT- MEDLINE
DCOM- 20160808
LR  - 20190221
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 34
IP  - 5
DP  - 2015 Nov
TI  - Identification of biomarkers with a tumor stage-dependent expression and 
      exploration of the mechanism involved in laryngeal squamous cell carcinoma.
PG  - 2627-35
LID - 10.3892/or.2015.4230 [doi]
AB  - The aim of this study was to identify biomarkers with a tumor stage-dependent 
      expression manner and explore the regulatory mechanisms of laryngeal squamous 
      cell carcinoma (LSCC) progression. Microarray data GSE59102 was used for 
      differential analysis using a limma package. Enrichment analyses were performed 
      for the differentially expressed genes (DEGs) between tumor tissues and normal 
      tissues at different stages. A co-expressed network involving the overlapped DEGs 
      in two stages was established based on Pearson's correlation coefficients. 
      Furthermore, for the tumor stage‑dependent expressed DEGs, a protein‑protein 
      interaction (PPI) network was constructed by mapping the genes using the STRING 
      database. Transcription factors (TFs), oncogenes and tumor‑associated genes 
      (TSGs) among the DEGs were predicted, following a search of the TRANSFAC, 
      tumor-associated gene (TAG) and TSG databases. The CDT database was used to 
      identify LSCC‑associated genes. In total, 696 DEGs from early stage and control 
      samples and 622 DEGs from advanced sttage and control samples were selected, 
      which were mainly enriched in the cell cycle pathway. In the co-expressed 
      network, BUB1, TTK, E2F1 and CEP55 were prominent, with E2F1 being predicted as a 
      TSG and CEP55 as an oncogene. The HOX family members were predicted as TFs. MMP1, 
      MMP9, MMP3 and PLAU were the most evident nodes in the PPI network, where MMP3 
      was connected with MMP1. The ADH family was correlated with LSCC. Several 
      biomarkers with tumor stage-dependent expression were identified including MMP1, 
      MMP3, MMP9, PLAU and ADHs. Additionally, the dysregulated cell cycle pathway 
      involving BUB1, TTK, E2F1 and CEP55, and the mediation of MMP1 by MMP3 as well as 
      the predicted TF HOX, may all play significant roles in LSCC progression.
FAU - Hui, Lian
AU  - Hui L
AD  - Department of Otolaryngology, The First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, P.R. China.
FAU - Yang, Ning
AU  - Yang N
AD  - Department of Otolaryngology, The First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, P.R. China.
FAU - Yang, Huijun
AU  - Yang H
AD  - Department of Otolaryngology, The First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, P.R. China.
FAU - Guo, Xing
AU  - Guo X
AD  - Department of Otolaryngology, The First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, P.R. China.
FAU - Jang, Xuejun
AU  - Jang X
AD  - Department of Otolaryngology, The First Hospital of China Medical University, 
      Shenyang, Liaoning 110001, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150827
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma, Squamous Cell/*genetics/*pathology
MH  - Computational Biology/methods
MH  - Disease Progression
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Regulatory Networks
MH  - Head and Neck Neoplasms/*genetics/*pathology
MH  - Humans
MH  - Neoplasm Staging
MH  - Oligonucleotide Array Sequence Analysis/methods
MH  - Squamous Cell Carcinoma of Head and Neck
EDAT- 2015/09/02 06:00
MHDA- 2016/08/09 06:00
CRDT- 2015/09/02 06:00
PHST- 2015/04/14 00:00 [received]
PHST- 2015/07/02 00:00 [accepted]
PHST- 2015/09/02 06:00 [entrez]
PHST- 2015/09/02 06:00 [pubmed]
PHST- 2016/08/09 06:00 [medline]
AID - 10.3892/or.2015.4230 [doi]
PST - ppublish
SO  - Oncol Rep. 2015 Nov;34(5):2627-35. doi: 10.3892/or.2015.4230. Epub 2015 Aug 27.