PMID- 26323510
OWN - NLM
STAT- MEDLINE
DCOM- 20170427
LR  - 20181113
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Print)
IS  - 1021-335X (Linking)
VI  - 34
IP  - 5
DP  - 2015 Nov
TI  - Immunotherapy for Lewis lung carcinoma utilizing dendritic cells infected with 
      CK19 gene recombinant adenoviral vectors.
PG  - 2289-95
LID - 10.3892/or.2015.4231 [doi]
AB  - Dendritic cells (DCs) as 'professional' antigen-presenting cells (APCs) initiate 
      and regulate immune responses to various antigens. DC-based vaccines have become 
      a promising modality in cancer immunotherapy. Cytokeratin 19 (CK19) protein is 
      expressed at high levels in lung cancer and many other tumor cells, suggesting 
      CK19 as a potential tumor‑specific target for cancer immune therapy. We 
      constructed a recombinant adenoviral vector containing the CK19 gene (rAd-CK19). 
      DCs transfected with rAd-CK19 were used to vaccinate C57BL/6 mice bearing 
      xenografts derived from Lewis lung carcinoma (LLC) cells. The transfected DCs 
      gave rise to potent CK19-specific cytotoxic T lymphocytes (CTLs) capable of 
      lysing LLC cells. Mice immunized with the rAd‑CK19-DCs exhibited significantly 
      attenuated tumor growth (including tumor volume and weight) when compared to the 
      tumor growth of mice immunized with rAd-c DCs or DCs during the 24-day 
      observation period (P<0.05). The results revealed that the mice vaccinated with 
      the rAd-CK19-DCs exhibited a potent protective and therapeutic antitumor immunity 
      to LLC cells in the subcutaneous model along with an inhibitive effect on tumor 
      growth compared to the mice vaccinated with the rAd-c DCs or DCs alone. The 
      present study proposes a meaningful mode of action utilizing rAd-CK19 DCs in lung 
      cancer immunotherapy.
FAU - Sun, Q F
AU  - Sun QF
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
FAU - Zhao, X N
AU  - Zhao XN
AD  - Department of Comprehensive Health Ⅱ, Provincial Hospital Affiliated to Shandong 
      University, Jinan, Shandong, P.R. China.
FAU - Peng, C L
AU  - Peng CL
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
FAU - Hao, Y T
AU  - Hao YT
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
FAU - Zhao, Y P
AU  - Zhao YP
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
FAU - Jiang, N
AU  - Jiang N
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
FAU - Xue, H
AU  - Xue H
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
FAU - Guo, J Z
AU  - Guo JZ
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
FAU - Yun, C H
AU  - Yun CH
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
FAU - Cong, B
AU  - Cong B
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
FAU - Zhao, X G
AU  - Zhao XG
AD  - Department of Thoracic Surgery, Second Hospital of Shandong University, Jinan, 
      Shandong, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150827
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (Cancer Vaccines)
RN  - 0 (Keratin-19)
SB  - IM
MH  - Adenoviridae/genetics
MH  - Animals
MH  - Antibody-Dependent Cell Cytotoxicity
MH  - Cancer Vaccines/genetics/*immunology
MH  - Carcinoma, Lewis Lung/immunology/*therapy
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Dendritic Cells/immunology/*transplantation
MH  - Gene Expression
MH  - Genetic Vectors
MH  - HEK293 Cells
MH  - Humans
MH  - Keratin-19/biosynthesis/genetics/*immunology
MH  - Mice, Inbred C57BL
MH  - Neoplasm Transplantation
MH  - T-Lymphocytes, Cytotoxic/immunology
MH  - Transduction, Genetic
PMC - PMC4583529
EDAT- 2015/09/02 06:00
MHDA- 2017/04/28 06:00
PMCR- 2015/08/27
CRDT- 2015/09/02 06:00
PHST- 2015/06/05 00:00 [received]
PHST- 2015/08/03 00:00 [accepted]
PHST- 2015/09/02 06:00 [entrez]
PHST- 2015/09/02 06:00 [pubmed]
PHST- 2017/04/28 06:00 [medline]
PHST- 2015/08/27 00:00 [pmc-release]
AID - or-34-05-2289 [pii]
AID - 10.3892/or.2015.4231 [doi]
PST - ppublish
SO  - Oncol Rep. 2015 Nov;34(5):2289-95. doi: 10.3892/or.2015.4231. Epub 2015 Aug 27.