PMID- 26323829
OWN - NLM
STAT- MEDLINE
DCOM- 20160808
LR  - 20220409
IS  - 1791-2431 (Electronic)
IS  - 1021-335X (Linking)
VI  - 34
IP  - 5
DP  - 2015 Nov
TI  - Hypoxia-induced fibroblast growth factor 11 stimulates capillary-like endothelial 
      tube formation.
PG  - 2745-51
LID - 10.3892/or.2015.4223 [doi]
AB  - Low oxygen or hypoxia can be observed in the central region of solid tumors. 
      Hypoxia is a strong stimulus for new blood vessel formation or angiogenesis, 
      which is essential for tumor growth and progression. Fibroblast growth factor 11 
      (FGF11) is an intracellular non-secretory FGF whose function has not yet been 
      fully characterized. In the present study, we demonstrated that FGF11 expression 
      is upregulated under hypoxic conditions in human umbilical vein endothelial cells 
      (HUVECs). FGF11 overexpression stimulated capillary-like tube formation, yet did 
      not affect cell migration. Notably, FGF11 markedly increased the levels of tight 
      junction proteins including occludin, zonula occludens-1 (ZO-1) and claudin-5 in 
      HUVECs. The FGF11 promoter contains hypoxia response elements (HREs), and 
      hypoxia-inducible factor-1 (HIF-1) binds to HREs to activate hypoxia-related 
      genes. We demonstrated that hypoxia or HIF-1 expression under normoxic conditions 
      increased the luciferase activity driven by the FGF11 promoter. However, deletion 
      of the HREs from the FGF11 promoter rendered reporter gene activity unresponsive 
      to hypoxia or HIF-1. Taken together, we propose that FGF11 may be involved in the 
      stabilization of capillary-like tube structures associated with angiogenesis and 
      may act as a modulator of hypoxia-induced pathological processes such as 
      tumorigenesis.
FAU - Yang, Jimin
AU  - Yang J
AD  - Biomedical Research Institute and IRICT, Seoul National University Hospital, 
      Seoul, Republic of Korea.
FAU - Kim, Woo Jean
AU  - Kim WJ
AD  - National Research Laboratory of Regenerative Sexual Medicine, Department of 
      Urology, Inha University School of Medicine, Incheon, Republic of Korea.
FAU - Jun, Hyoung Oh
AU  - Jun HO
AD  - Biomedical Research Institute, Seoul National University Hospital, Seoul, 
      Republic of Korea.
FAU - Lee, Eun Ju
AU  - Lee EJ
AD  - Biomedical Research Institute and IRICT, Seoul National University Hospital, 
      Seoul, Republic of Korea.
FAU - Lee, Kyeong Won
AU  - Lee KW
AD  - Marine Biotechnology Research Group, Korea Institute of Ocean Science and 
      Technology, Ansan, Republic of Korea.
FAU - Jeong, Jae-Yeon
AU  - Jeong JY
AD  - Marine Biotechnology Research Group, Korea Institute of Ocean Science and 
      Technology, Ansan, Republic of Korea.
FAU - Lee, Sae-Won
AU  - Lee SW
AD  - Biomedical Research Institute and IRICT, Seoul National University Hospital, 
      Seoul, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150824
PL  - Greece
TA  - Oncol Rep
JT  - Oncology reports
JID - 9422756
RN  - 0 (CLDN5 protein, human)
RN  - 0 (Claudin-5)
RN  - 0 (FGF11 protein, human)
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (TJP1 protein, human)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 62031-54-3 (Fibroblast Growth Factors)
SB  - IM
MH  - Binding Sites
MH  - Cell Hypoxia
MH  - Cell Movement
MH  - Claudin-5/metabolism
MH  - Endothelial Cells/*cytology/metabolism
MH  - Fibroblast Growth Factors/chemistry/*genetics/*metabolism
MH  - HEK293 Cells
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*metabolism
MH  - Promoter Regions, Genetic
MH  - Up-Regulation
MH  - Zonula Occludens-1 Protein/metabolism
EDAT- 2015/09/02 06:00
MHDA- 2016/08/09 06:00
CRDT- 2015/09/02 06:00
PHST- 2015/06/04 00:00 [received]
PHST- 2015/07/06 00:00 [accepted]
PHST- 2015/09/02 06:00 [entrez]
PHST- 2015/09/02 06:00 [pubmed]
PHST- 2016/08/09 06:00 [medline]
AID - 10.3892/or.2015.4223 [doi]
PST - ppublish
SO  - Oncol Rep. 2015 Nov;34(5):2745-51. doi: 10.3892/or.2015.4223. Epub 2015 Aug 24.