PMID- 26323841 OWN - NLM STAT- MEDLINE DCOM- 20160316 LR - 20220331 IS - 1738-0006 (Electronic) IS - 0023-4001 (Print) IS - 0023-4001 (Linking) VI - 53 IP - 4 DP - 2015 Aug TI - Toll-Like Receptor Gene Expression during Trichinella spiralis Infection. PG - 431-8 LID - 10.3347/kjp.2015.53.4.431 [doi] AB - In Trichinella spiralis infection, type 2 helper T (Th2) cell-related and regulatory T (Treg) cell-related immune responses are the most important immune events. In order to clarify which Toll-like receptors (TLRs) are closely associated with these responses, we analyzed the expression of mouse TLR genes in the small intestine and muscle tissue during T. spiralis infection. In addition, the expression of several chemokine- and cytokine-encoding genes, which are related to Th2 and Treg cell mediated immune responses, were analyzed in mouse embryonic fibroblasts (MEFs) isolated from myeloid differentiation factor 88 (MyD88)/TIR-associated proteins (TIRAP) and Toll receptor-associated activator of interferons (TRIF) adapter protein deficient and wild type (WT) mice. The results showed significantly increased TLR4 and TLR9 gene expression in the small intestine after 2 weeks of T. spiralis infection. In the muscle, TLR1, TLR2, TLR5, and TLR9 gene expression significantly increased after 4 weeks of infection. Only the expression of the TLR4 and TLR9 genes was significantly elevated in WT MEF cells after treatment with excretory-secretory (ES) proteins. Gene expression for Th2 chemokine genes were highly enhanced by ES proteins in WT MEF cells, while this elevation was slightly reduced in MyD88/TIRAP(-/-) MEF cells, and quite substantially decreased in TRIF(-/-) MEF cells. In contrast, IL-10 and TGF-beta expression levels were not elevated in MyD88/TIRAP(-/-) MEF cells. In conclusion, we suggest that TLR4 and TLR9 might be closely linked to Th2 cell and Treg cell mediated immune responses, although additional data are needed to convincingly prove this observation. FAU - Kim, Sin AU - Kim S AD - Department of Parasitology, School of Medicine, Pusan National University, Yangsan 626-770, Korea. AD - Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Yangsan 626-770, Korea. FAU - Park, Mi Kyung AU - Park MK AD - Department of Parasitology, School of Medicine, Pusan National University, Yangsan 626-770, Korea. AD - Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Yangsan 626-770, Korea. FAU - Yu, Hak Sun AU - Yu HS AD - Department of Parasitology, School of Medicine, Pusan National University, Yangsan 626-770, Korea. AD - Immunoregulatory Therapeutics Group in Brain Busan 21 Project, Yangsan 626-770, Korea. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150825 PL - Korea (South) TA - Korean J Parasitol JT - The Korean journal of parasitology JID - 9435800 RN - 0 (Toll-Like Receptors) RN - 130068-27-8 (Interleukin-10) SB - IM MH - Animals MH - Gene Expression MH - Humans MH - Interleukin-10/genetics MH - Mice MH - Mice, Knockout MH - Th2 Cells/metabolism MH - Toll-Like Receptors/*genetics/metabolism MH - Trichinella spiralis/genetics/*physiology MH - Trichinellosis/genetics/metabolism/*parasitology PMC - PMC4566501 OTO - NOTNLM OT - Toll-like receptor 9 OT - Trichinella spiralis OT - intestinal phase OT - muscle phase COIS- We have no conflict of interest related to this work. EDAT- 2015/09/02 06:00 MHDA- 2016/03/17 06:00 PMCR- 2015/08/01 CRDT- 2015/09/02 06:00 PHST- 2015/04/21 00:00 [received] PHST- 2015/06/22 00:00 [revised] PHST- 2015/07/12 00:00 [accepted] PHST- 2015/09/02 06:00 [entrez] PHST- 2015/09/02 06:00 [pubmed] PHST- 2016/03/17 06:00 [medline] PHST- 2015/08/01 00:00 [pmc-release] AID - kjp-53-4-431 [pii] AID - 10.3347/kjp.2015.53.4.431 [doi] PST - ppublish SO - Korean J Parasitol. 2015 Aug;53(4):431-8. doi: 10.3347/kjp.2015.53.4.431. Epub 2015 Aug 25.