PMID- 26327113
OWN - NLM
STAT- MEDLINE
DCOM- 20160815
LR  - 20151103
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 47
IP  - 1
DP  - 2015 Nov
TI  - Flavonoid fraction of guava leaf extract attenuates lipopolysaccharide-induced 
      inflammatory response via blocking of NF-kappaB signalling pathway in Labeo rohita 
      macrophages.
PG  - 85-92
LID - S1050-4648(15)30121-2 [pii]
LID - 10.1016/j.fsi.2015.08.031 [doi]
AB  - Psidium guajava L. is a well-known traditional medicinal plant widely used in 
      folk medicine. To explore the anti-inflammatory activity of the flavonoid 
      fraction of guava leaf extract (FGLE), we investigated its ability to suppress 
      the levels of inflammatory mediators elevated by lipopolysaccharide (LPS) in 
      Labeo rohita head-kidney (HK) macrophages. HK macrophages of L. rohita were 
      treated with LPS in the presence or absence of the FGLE. We examined the 
      inhibitory effect of FGLE on LPS-induced nitric oxide (NO) and prostaglandin E2 
      (PGE2) production. The inhibitory effect of FGLE on nitric oxide synthase (iNOS) 
      and cyclooxygenase-2 (COX-2) were investigated by RT-PCR and western blot. The 
      effect of FGLE on proinflammatory cytokines tumour necrosis factor alpha (TNF-alpha) 
      or interleukin-1beta (IL-1beta) was also investigated by ELISA and RT-PCR. The 
      phosphorylation of three mitogen activated protein kinases (MAPK) molecules ERK, 
      JNK and p38 was analysed by western blot analysis. FGLE inhibited LPS-induced NO 
      and PGE2 production. It also effectively inhibited TNF-alpha, IL-1beta, IL-10, iNOS, and 
      COX-2 production in a concentration-dependent manner. In addition, FGLE 
      suppressed the mRNA expression levels of TNF-alpha and IL-1beta in LPS-stimulated HK 
      macrophages. RT-PCR and western blot analysis showed that FGLE decreased both the 
      mRNA and protein expression levels of LPS-induced iNOS and COX-2 in HK 
      macrophages. FGLE suppresses the phosphorylation of MAPK molecules in 
      LPS-stimulated HK macrophages. FGLE also significantly inhibited LPS-induced 
      NF-kappaB transcriptional activity. The molecular mechanism by which FGLE suppresses 
      the expression of inflammatory mediators appears to involve the inhibition of 
      NF-kappaB activation, through the suppression of LPS-induced IkappaB-alpha degradation. 
      Together these results suggest that FGLE contains potential therapeutic agent(s), 
      which regulate NF-kappaB activation, for the treatment of inflammatory conditions in 
      L. rohita macrophages.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Sen, Shib Sankar
AU  - Sen SS
AD  - School of Life Sciences, Jawharlal Nehru University, New Delhi-110067 Delhi, 
      India. Electronic address: shibsankar.iicb@gmail.com.
FAU - Sukumaran, V
AU  - Sukumaran V
AD  - Dept. of Biotechnology, Periyar Maniammai University, Thanjavur-613403, Tamil 
      Nadu, India. Electronic address: drvsukumar@gmail.com.
FAU - Giri, Sib Sankar
AU  - Giri SS
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul-151742, South 
      Korea. Electronic address: giribiotek@gmail.com.
FAU - Park, Se Chang
AU  - Park SC
AD  - Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research 
      Institute for Veterinary Science, Seoul National University, Seoul-151742, South 
      Korea. Electronic address: parksec@snu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150829
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Flavonoids)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Animals
MH  - *Cyprinidae
MH  - Fish Diseases/genetics/*immunology/microbiology
MH  - Flavonoids/*pharmacology
MH  - Head Kidney/immunology
MH  - Inflammation/microbiology/*veterinary
MH  - Lipopolysaccharides/*physiology
MH  - Macrophages/immunology
MH  - NF-kappa B/*genetics/metabolism
MH  - Plant Extracts/pharmacology
MH  - Plant Leaves/chemistry
MH  - Psidium/*chemistry
MH  - Signal Transduction
OTO - NOTNLM
OT  - FGLE
OT  - Labeo rohita
OT  - Lipopolysaccharide
OT  - Macrophages
OT  - NF-kappaB signalling pathway
EDAT- 2015/09/04 06:00
MHDA- 2016/08/16 06:00
CRDT- 2015/09/02 06:00
PHST- 2015/05/07 00:00 [received]
PHST- 2015/08/21 00:00 [revised]
PHST- 2015/08/26 00:00 [accepted]
PHST- 2015/09/02 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2016/08/16 06:00 [medline]
AID - S1050-4648(15)30121-2 [pii]
AID - 10.1016/j.fsi.2015.08.031 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2015 Nov;47(1):85-92. doi: 10.1016/j.fsi.2015.08.031. 
      Epub 2015 Aug 29.