PMID- 26327218
OWN - NLM
STAT- MEDLINE
DCOM- 20151028
LR  - 20181202
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 102
DP  - 2015 Aug 14
TI  - Analysis of Developing Tooth Germ Innervation Using Microfluidic Co-culture 
      Devices.
PG  - e53114
LID - 10.3791/53114 [doi]
LID - 53114
AB  - Innervation plays a key role in the development, homeostasis and regeneration of 
      organs and tissues. However, the mechanisms underlying these phenomena are not 
      well understood yet. In particular, the role of innervation in tooth development 
      and regeneration is neglected. Several in vivo studies have provided important 
      information about the patterns of innervation of dental tissues during 
      development and repair processes of various animal models. However, most of these 
      approaches are not optimal to highlight the molecular basis of the interactions 
      between nerve fibres and target organs and tissues. Co-cultures constitute a 
      valuable method to investigate and manipulate the interactions between nerve 
      fibres and teeth in a controlled and isolated environment. In the last decades, 
      conventional co-cultures using the same culture medium have been performed for 
      very short periods (e.g., two days) to investigate the attractive or repulsive 
      effects of developing oral and dental tissues on sensory nerve fibres. However, 
      extension of the culture period is required to investigate the effects of 
      innervation on tooth morphogenesis and cytodifferentiation. Microfluidics systems 
      allow co-cultures of neurons and different cell types in their appropriate 
      culture media. We have recently demonstrated that trigeminal ganglia (TG) and 
      teeth are able to survive for a long period of time when co-cultured in 
      microfluidic devices, and that they maintain in these conditions the same 
      innervation pattern that they show in vivo. On this basis, we describe how to 
      isolate and co-culture developing trigeminal ganglia and tooth germs in a 
      microfluidic co-culture system.This protocol describes a simple and flexible way 
      to co-culture ganglia/nerves and target tissues and to study the roles of 
      specific molecules on such interactions in a controlled and isolated environment.
FAU - Pagella, Pierfrancesco
AU  - Pagella P
AD  - Institute of Oral Biology, Unit of Orofacial Development and Regeneration, 
      University of Zurich.
FAU - Miran, Shayee
AU  - Miran S
AD  - Institute of Oral Biology, Unit of Orofacial Development and Regeneration, 
      University of Zurich.
FAU - Mitsiadis, Tim
AU  - Mitsiadis T
AD  - Institute of Oral Biology, Unit of Orofacial Development and Regeneration, 
      University of Zurich; thimios.mitsiadis@zzm.uzh.ch.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20150814
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - Coculture Techniques/instrumentation/*methods
MH  - Female
MH  - Mice
MH  - Microfluidic Analytical Techniques/instrumentation/*methods
MH  - Pregnancy
MH  - Tooth Germ/*cytology/*innervation
MH  - Trigeminal Ganglion/*cytology
PMC - PMC4692442
EDAT- 2015/09/04 06:00
MHDA- 2015/10/29 06:00
PMCR- 2017/08/14
CRDT- 2015/09/02 06:00
PHST- 2015/09/02 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2015/10/29 06:00 [medline]
PHST- 2017/08/14 00:00 [pmc-release]
AID - 53114 [pii]
AID - 10.3791/53114 [doi]
PST - epublish
SO  - J Vis Exp. 2015 Aug 14;(102):e53114. doi: 10.3791/53114.