PMID- 26328014
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150902
LR  - 20201001
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 7
IP  - 7
DP  - 2015
TI  - Characterization of Der f 29, a new allergen from dermatophagoides farinae.
PG  - 1303-13
AB  - More than 30 allergens have been identified from Dermatophagoides farina (D. 
      farina), which is one of the main species of house dust mites. The mite allergens 
      are an important factor contributing to allergic disease in the world. As the 
      detection and identification of new allergens is critical for the diagnosis or 
      treatment of allergic diseases, we sought to characterize the profilin of D. 
      farina (Der f 29) in this study. The results showed that 21% of allergic patients 
      displayed positive results in skin prick test with recombinant Der f 29 (rDer f 
      29) as the specific allergen; specific IgE reactivity to rDer f 29 was shown by 
      Western Blot and ELISA. In addition, rDer f 29 induced bone marrow-derived 
      dendritic cells (DC) to produce T cells immunoglobulin domain and mucin domain 
      protein 4 (TIM4). Moreover, an allergic asthma mouse model was established by 
      challenging with rDer f 29. Airway hyperresponsiveness, serum specific IgE, IgG1, 
      eosinophil infiltration in the allergic mice bronchoalveolar lavage fluid, the 
      cytokines interleukin-4 (IL-4) and interferon-gamma (INF-gamma) from spleen cells were 
      markedly increased; the histology showed severe inflammation in the lung. In 
      conclusion, Der f 29 is identified as a new type of the house dust mite allergen.
FAU - Jiang, Congli
AU  - Jiang C
AD  - Key Laboratory of Respiratory Disease for Allergy at Shengzhen University; School 
      of Medicine, Shenzhen University 3688 Nanhai Ave., Shenzhen 518060, Guangdong, P. 
      R. China.
FAU - Fan, Xiaoqin
AU  - Fan X
AD  - Shenzhen Longgang Central Hospital, ENT Institute Shenzhen, Guangdong, P. R. 
      China.
FAU - Li, Meng
AU  - Li M
AD  - Key Laboratory of Respiratory Disease for Allergy at Shengzhen University; School 
      of Medicine, Shenzhen University 3688 Nanhai Ave., Shenzhen 518060, Guangdong, P. 
      R. China.
FAU - Xing, Peng
AU  - Xing P
AD  - Key Laboratory of Respiratory Disease for Allergy at Shengzhen University; School 
      of Medicine, Shenzhen University 3688 Nanhai Ave., Shenzhen 518060, Guangdong, P. 
      R. China.
FAU - Liu, Xiaoyu
AU  - Liu X
AD  - Key Laboratory of Respiratory Disease for Allergy at Shengzhen University; School 
      of Medicine, Shenzhen University 3688 Nanhai Ave., Shenzhen 518060, Guangdong, P. 
      R. China.
FAU - Wu, Yulan
AU  - Wu Y
AD  - Key Laboratory of Respiratory Disease for Allergy at Shengzhen University; School 
      of Medicine, Shenzhen University 3688 Nanhai Ave., Shenzhen 518060, Guangdong, P. 
      R. China.
FAU - Zhang, Min
AU  - Zhang M
AD  - Key Laboratory of Respiratory Disease for Allergy at Shengzhen University; School 
      of Medicine, Shenzhen University 3688 Nanhai Ave., Shenzhen 518060, Guangdong, P. 
      R. China.
FAU - Yang, Pingchang
AU  - Yang P
AD  - Key Laboratory of Respiratory Disease for Allergy at Shengzhen University; School 
      of Medicine, Shenzhen University 3688 Nanhai Ave., Shenzhen 518060, Guangdong, P. 
      R. China.
FAU - Liu, Zhigang
AU  - Liu Z
AD  - Key Laboratory of Respiratory Disease for Allergy at Shengzhen University; School 
      of Medicine, Shenzhen University 3688 Nanhai Ave., Shenzhen 518060, Guangdong, P. 
      R. China.
LA  - eng
PT  - Journal Article
DEP - 20150715
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC4548322
OTO - NOTNLM
OT  - Profilin
OT  - dendritic cell
OT  - der f 29
OT  - expression
OT  - purification
EDAT- 2015/09/04 06:00
MHDA- 2015/09/04 06:01
PMCR- 2015/07/15
CRDT- 2015/09/02 06:00
PHST- 2015/03/02 00:00 [received]
PHST- 2015/07/11 00:00 [accepted]
PHST- 2015/09/02 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2015/09/04 06:01 [medline]
PHST- 2015/07/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2015 Jul 15;7(7):1303-13. eCollection 2015.