PMID- 26330558
OWN - NLM
STAT- MEDLINE
DCOM- 20160208
LR  - 20210205
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 290
IP  - 42
DP  - 2015 Oct 16
TI  - Myosin IIb-dependent Regulation of Actin Dynamics Is Required for 
      N-Methyl-D-aspartate Receptor Trafficking during Synaptic Plasticity.
PG  - 25395-410
LID - 10.1074/jbc.M115.644229 [doi]
AB  - N-Methyl-d-aspartate receptor (NMDAR) synaptic incorporation changes the number 
      of NMDARs at synapses and is thus critical to various NMDAR-dependent brain 
      functions. To date, the molecules involved in NMDAR trafficking and the 
      underlying mechanisms are poorly understood. Here, we report that myosin IIb is 
      an essential molecule in NMDAR synaptic incorporation during PKC- or theta burst 
      stimulation-induced synaptic plasticity. Moreover, we demonstrate that myosin 
      light chain kinase (MLCK)-dependent actin reorganization contributes to NMDAR 
      trafficking. The findings from additional mutual occlusion experiments 
      demonstrate that PKC and MLCK share a common signaling pathway in NMDAR-mediated 
      synaptic regulation. Because myosin IIb is the primary substrate of MLCK and can 
      regulate actin dynamics during synaptic plasticity, we propose that the MLCK- and 
      myosin IIb-dependent regulation of actin dynamics is required for NMDAR 
      trafficking during synaptic plasticity. This study provides important insights 
      into a mechanical framework for understanding NMDAR trafficking associated with 
      synaptic plasticity.
CI  - (c) 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
FAU - Bu, Yunfei
AU  - Bu Y
AD  - From the Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu 
      Province 210029, China.
FAU - Wang, Ning
AU  - Wang N
AD  - From the Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu 
      Province 210029, China, the Key Laboratory of Developmental Genes and Human 
      Disease of the Ministry of Education of China, Institute of Life Sciences, 
      Southeast University, Nanjing, Jiangsu Province 210096, China.
FAU - Wang, Shaoli
AU  - Wang S
AD  - From the Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu 
      Province 210029, China, the Key Laboratory of Developmental Genes and Human 
      Disease of the Ministry of Education of China, Institute of Life Sciences, 
      Southeast University, Nanjing, Jiangsu Province 210096, China.
FAU - Sheng, Tao
AU  - Sheng T
AD  - From the Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu 
      Province 210029, China, the Key Laboratory of Developmental Genes and Human 
      Disease of the Ministry of Education of China, Institute of Life Sciences, 
      Southeast University, Nanjing, Jiangsu Province 210096, China.
FAU - Tian, Tian
AU  - Tian T
AD  - From the Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu 
      Province 210029, China.
FAU - Chen, Linlin
AU  - Chen L
AD  - From the Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu 
      Province 210029, China.
FAU - Pan, Weiwei
AU  - Pan W
AD  - From the Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu 
      Province 210029, China.
FAU - Zhu, Minsheng
AU  - Zhu M
AD  - the Key Laboratory of Model Animal for Disease Study of the Ministry of Education 
      of China, Model Animal Research Center, Nanjing University, Nanjing, Jiangsu 
      Province 210063, China, and.
FAU - Luo, Jianhong
AU  - Luo J
AD  - the Department of Neurobiology, Key Laboratory of Medical Neurobiology of the 
      Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology, 
      Zhejiang University School of Medicine, Hangzhou, Zhejiang Province 310058, 
      China.
FAU - Lu, Wei
AU  - Lu W
AD  - From the Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu 
      Province 210029, China, the Key Laboratory of Developmental Genes and Human 
      Disease of the Ministry of Education of China, Institute of Life Sciences, 
      Southeast University, Nanjing, Jiangsu Province 210096, China, the Co-innovation 
      Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province 
      226001, China, luwei@seu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150901
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Actins)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.18 (Myosin-Light-Chain Kinase)
RN  - EC 3.6.1.- (Nonmuscle Myosin Type IIB)
SB  - IM
MH  - Actins/*physiology
MH  - Animals
MH  - Enzyme Activation
MH  - Hippocampus/physiology
MH  - In Vitro Techniques
MH  - Mice
MH  - Mice, Knockout
MH  - Myosin-Light-Chain Kinase/genetics/metabolism
MH  - *Neuronal Plasticity
MH  - Nonmuscle Myosin Type IIB/*physiology
MH  - Protein Kinase C/metabolism
MH  - Protein Transport
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
PMC - PMC4646188
OTO - NOTNLM
OT  - NMDA receptor
OT  - actin
OT  - myosin
OT  - myosin IIb
OT  - patch clamp
OT  - protein kinase C (PKC)
OT  - synaptic plasticity
OT  - trafficking
EDAT- 2015/09/04 06:00
MHDA- 2016/02/09 06:00
PMCR- 2016/10/16
CRDT- 2015/09/03 06:00
PHST- 2015/02/10 00:00 [received]
PHST- 2015/09/03 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2016/02/09 06:00 [medline]
PHST- 2016/10/16 00:00 [pmc-release]
AID - S0021-9258(20)44559-4 [pii]
AID - M115.644229 [pii]
AID - 10.1074/jbc.M115.644229 [doi]
PST - ppublish
SO  - J Biol Chem. 2015 Oct 16;290(42):25395-410. doi: 10.1074/jbc.M115.644229. Epub 
      2015 Sep 1.