PMID- 26332363
OWN - NLM
STAT- MEDLINE
DCOM- 20170123
LR  - 20190221
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 45
IP  - 3
DP  - 2016 Mar
TI  - Anticancer activity of Ashwagandha against human head and neck cancer cell lines.
PG  - 193-201
LID - 10.1111/jop.12353 [doi]
AB  - BACKGROUND: The aim of this study was to determine the apoptotic activity of 
      methanol extract of Ashwagandha (MEAG) and in human head and neck squamous cell 
      carcinoma (HNSCC) cells and to investigate the underlying mechanisms. METHODS: We 
      investigated the effects of MEAG on programmed cell death in HNSCC cells using a 
      Live/Dead assay, detection of nuclear morphologic changes, Mitotracker, siRNA 
      knockdown, and RT-PCR. RESULTS: Treatment with MEAG showed dose-dependent 
      growth-inhibitory activity that attribute to caspase-dependent apoptosis. Loss of 
      mitochondrial membrane potential, release of cytochrome c, and activation of 
      caspase 9 suggested that MEAG leads to activation of mitochondria-mediated 
      apoptosis. MEAG selectively upregulated the expression of Bim protein at the 
      transcriptional level and induced the translocation of Bim into the mitochondria. 
      Knockdown of Bim by siRNA partially blocked MEAG-mediated apoptosis. MEAG also 
      caused an increase in truncated Bid (t-Bid), cleaved caspase-8, and death 
      receptor 5 (DR5). Interestingly, withaferin A (WA), a bioactive component of 
      MEAG, clearly induced apoptosis accompanied by upregulation of Bim, t-Bid, 
      caspase-8, and DR5 similar to the effects of MEAG. CONCLUSIONS: These suggest 
      that MEAG and WA may be potential natural materials for the treatment of HNSCC.
CI  - (c) 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Lee, Haeng-Eun
AU  - Lee HE
AD  - Department of Oral Pathology, School of Dentistry and Institute of Biodegradable 
      Material, Institute of Oral Bioscience, Brain Korea 21 Project, Chonbuk National 
      University, Jeon-ju, Korea.
FAU - Shin, Ji-Ae
AU  - Shin JA
AD  - Department of Oral Pathology, School of Dentistry and Institute of Biodegradable 
      Material, Institute of Oral Bioscience, Brain Korea 21 Project, Chonbuk National 
      University, Jeon-ju, Korea.
FAU - Jeong, Joseph H
AU  - Jeong JH
AD  - Department of Dermatology and Biochemistry, Medical College of Wisconsin, 
      Milwaukee, WI, USA.
FAU - Jeon, Jae-Gyu
AU  - Jeon JG
AD  - Department of Preventive Dentistry, School of Dentistry and Institute of Oral 
      Bioscience, Chonbuk National University, Jeonju, Korea.
FAU - Lee, Min-Ho
AU  - Lee MH
AD  - Department of Dental Biomaterials and Institute of Biodegradable Material, 
      Institute of Oral Bioscience and BK21 Plus Project, School of Dentistry, Chonbuk 
      National University, Jeonju, Korea.
FAU - Cho, Sung-Dae
AU  - Cho SD
AD  - Department of Oral Pathology, School of Dentistry and Institute of Biodegradable 
      Material, Institute of Oral Bioscience, Brain Korea 21 Project, Chonbuk National 
      University, Jeon-ju, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150831
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Bcl-2-Like Protein 11)
RN  - 0 (Plant Extracts)
RN  - 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand)
RN  - 0 (TNFRSF10B protein, human)
RN  - 0 (Withanolides)
RN  - 9007-43-6 (Cytochromes c)
RN  - EC 3.4.22.- (Caspase 8)
RN  - EC 3.4.22.- (Caspase 9)
RN  - L6DO3QW4K5 (withaferin A)
RN  - V038D626IF (Ashwagandha)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis/drug effects
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Bcl-2-Like Protein 11/deficiency/genetics/metabolism
MH  - Carcinoma, Squamous Cell/*drug therapy/metabolism/pathology
MH  - Caspase 8/metabolism
MH  - Caspase 9/drug effects/metabolism
MH  - Cell Line, Tumor
MH  - Cytochromes c/metabolism
MH  - Enzyme Activation/drug effects
MH  - Head and Neck Neoplasms/*drug therapy/metabolism/pathology
MH  - Humans
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mitochondrial Membranes/drug effects
MH  - Mouth Neoplasms/drug therapy
MH  - Plant Extracts/*pharmacology
MH  - Receptors, TNF-Related Apoptosis-Inducing Ligand/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck
MH  - Up-Regulation
MH  - Withanolides/pharmacology
OTO - NOTNLM
OT  - Ashwagandha
OT  - apoptosis
OT  - head and neck cancer
EDAT- 2015/09/04 06:00
MHDA- 2017/01/24 06:00
CRDT- 2015/09/03 06:00
PHST- 2015/07/27 00:00 [accepted]
PHST- 2015/09/03 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2017/01/24 06:00 [medline]
AID - 10.1111/jop.12353 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2016 Mar;45(3):193-201. doi: 10.1111/jop.12353. Epub 2015 Aug 
      31.