PMID- 26332577
OWN - NLM
STAT- MEDLINE
DCOM- 20160325
LR  - 20150923
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 63
IP  - 37
DP  - 2015 Sep 23
TI  - Study on the Immunoreactivity of Triticum monococcum (Einkorn) Wheat in Patients 
      with Wheat-Dependent Exercise-Induced Anaphylaxis for the Production of 
      Hypoallergenic Foods.
PG  - 8299-306
LID - 10.1021/acs.jafc.5b02648 [doi]
AB  - Wheat [Triticum aestivum (T.a.)] ingestion can cause a specific allergic 
      reaction, which is called wheat-dependent exercise-induced anaphylaxis (WDEIA). 
      The major allergen involved is omega-5 gliadin, a gluten protein coded by genes 
      located on the B genome. Our aim was to study the immunoreactivity of proteins in 
      Triticum monococcum (einkorn, T.m.), a diploid ancestral wheat lacking B 
      chromosomes, for possible use in the production of hypoallergenic foods. A total 
      of 14 patients with a clear history of WDEIA and specific immunoglobulin E (IgE) 
      to omega-5 gliadin were enrolled. Skin prick test (SPT) with a commercial wheat 
      extract and an in-house T.a. gluten diagnostic solution tested positive for 43 
      and 100% of the cases, respectively. No reactivity in patients tested with 
      solutions prepared from four T.m. accessions was observed. The immunoblotting of 
      T.m. gluten proteins performed with the sera of patients showed different 
      IgE-binding profiles with respect to T.a., confirming the absence of omega-5 gliadin. 
      A general lower immunoreactivity of T.m. gluten proteins with scarce 
      cross-reactivity to omega-5 gliadin epitopes was assessed by an enzyme-linked 
      immunosorbent assay (ELISA). Given the absence of reactivity by SPT and the 
      limited cross-reactivity with omega-5 gliadin, T.m. might represent a potential 
      candidate in the production of hypoallergenic bakery products for patients 
      sensitized to omega-5 gliadin. Further analyses need to be carried out regarding its 
      safety.
FAU - Lombardo, Carla
AU  - Lombardo C
FAU - Bolla, Michela
AU  - Bolla M
AD  - Department of Biotechnology, University of Verona , 37134 Verona, Italy.
FAU - Chignola, Roberto
AU  - Chignola R
AD  - Department of Biotechnology, University of Verona , 37134 Verona, Italy.
FAU - Senna, Gianenrico
AU  - Senna G
FAU - Rossin, Giacomo
AU  - Rossin G
AD  - Department of Biotechnology, University of Verona , 37134 Verona, Italy.
FAU - Caruso, Beatrice
AU  - Caruso B
FAU - Tomelleri, Carlo
AU  - Tomelleri C
AD  - Department of Biotechnology, University of Verona , 37134 Verona, Italy.
FAU - Cecconi, Daniela
AU  - Cecconi D
AD  - Department of Biotechnology, University of Verona , 37134 Verona, Italy.
FAU - Brandolini, Andrea
AU  - Brandolini A
AD  - CRA-SCV, Sant'Angelo Lodigiano , 26866 Lodi, Italy.
FAU - Zoccatelli, Gianni
AU  - Zoccatelli G
AD  - Department of Biotechnology, University of Verona , 37134 Verona, Italy.
LA  - eng
PT  - Journal Article
DEP - 20150911
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Allergens)
RN  - 0 (Antigens, Plant)
RN  - 0 (Epitopes)
RN  - 0 (Plant Extracts)
RN  - 0 (omega-5 gliadin, wheat)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 8002-80-0 (Glutens)
RN  - 9007-90-3 (Gliadin)
SB  - IM
MH  - Adult
MH  - Allergens/analysis/immunology
MH  - Anaphylaxis/*immunology
MH  - Antigens, Plant/genetics
MH  - Chromosomes, Plant
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epitopes/immunology
MH  - *Exercise
MH  - Female
MH  - Flour/analysis
MH  - Gliadin/genetics
MH  - Glutens/analysis/*immunology
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Male
MH  - Middle Aged
MH  - Plant Extracts/immunology
MH  - Skin Tests
MH  - Triticum/genetics/*immunology
MH  - Wheat Hypersensitivity/*immunology/*prevention & control
OTO - NOTNLM
OT  - Triticum monococcum
OT  - allergy
OT  - wheat
OT  - wheat-dependent exercise-induced anaphylaxis (WDEIA)
OT  - omega gliadin
EDAT- 2015/09/04 06:00
MHDA- 2016/03/26 06:00
CRDT- 2015/09/03 06:00
PHST- 2015/09/03 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2016/03/26 06:00 [medline]
AID - 10.1021/acs.jafc.5b02648 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2015 Sep 23;63(37):8299-306. doi: 10.1021/acs.jafc.5b02648. 
      Epub 2015 Sep 11.