PMID- 26333092
OWN - NLM
STAT- MEDLINE
DCOM- 20160523
LR  - 20240327
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 9
DP  - 2015
TI  - Fluorescence In Situ Hybridization (FISH) Assays for Diagnosing Malaria in 
      Endemic Areas.
PG  - e0136726
LID - 10.1371/journal.pone.0136726 [doi]
LID - e0136726
AB  - Malaria is a responsible for approximately 600 thousand deaths worldwide every 
      year. Appropriate and timely treatment of malaria can prevent deaths but is 
      dependent on accurate and rapid diagnosis of the infection. Currently, 
      microscopic examination of the Giemsa stained blood smears is the method of 
      choice for diagnosing malaria. Although it has limited sensitivity and 
      specificity in field conditions, it still remains the gold standard for the 
      diagnosis of malaria. Here, we report the development of a fluorescence in situ 
      hybridization (FISH) based method for detecting malaria infection in blood smears 
      and describe the use of an LED light source that makes the method suitable for 
      use in resource-limited malaria endemic countries. The Plasmodium Genus (P-Genus) 
      FISH assay has a Plasmodium genus specific probe that detects all five species of 
      Plasmodium known to cause the disease in humans. The P. falciparum (PF) FISH 
      assay and P. vivax (PV) FISH assay detect and differentiate between P. falciparum 
      and P. vivax respectively from other Plasmodium species. The FISH assays are more 
      sensitive than Giemsa. The sensitivities of P-Genus, PF and PV FISH assays were 
      found to be 98.2%, 94.5% and 98.3%, respectively compared to 89.9%, 83.3% and 
      87.9% for the detection of Plasmodium, P. falciparum and P. vivax by Giemsa 
      staining respectively.
FAU - Shah, Jyotsna
AU  - Shah J
AD  - ID-FISH Technology Inc., Palo Alto, California, United States of America; IGeneX 
      Inc. Palo Alto, California, United States of America.
FAU - Mark, Olivia
AU  - Mark O
AD  - ID-FISH Technology Inc., Palo Alto, California, United States of America; IGeneX 
      Inc. Palo Alto, California, United States of America.
FAU - Weltman, Helena
AU  - Weltman H
AD  - ID-FISH Technology Inc., Palo Alto, California, United States of America; IGeneX 
      Inc. Palo Alto, California, United States of America.
FAU - Barcelo, Nicolas
AU  - Barcelo N
AD  - ID-FISH Technology Inc., Palo Alto, California, United States of America.
FAU - Lo, Wai
AU  - Lo W
AD  - IGeneX Inc. Palo Alto, California, United States of America.
FAU - Wronska, Danuta
AU  - Wronska D
AD  - IGeneX Inc. Palo Alto, California, United States of America.
FAU - Kakkilaya, Srinivas
AU  - Kakkilaya S
AD  - Nova Meditech Pvt. Ltd., Mangalore, India.
FAU - Rao, Aravinda
AU  - Rao A
AD  - Nova Meditech Pvt. Ltd., Mangalore, India.
FAU - Bhat, Shalia T
AU  - Bhat ST
AD  - Kasturba Medical College Hospital, Mangalore, India.
FAU - Sinha, Ruchi
AU  - Sinha R
AD  - Kasturba Medical College Hospital, Mangalore, India.
FAU - Omar, Sabah
AU  - Omar S
AD  - Kenya Medical Research Institute, Nairobi, Kenya.
FAU - O'bare, Peter
AU  - O'bare P
AD  - Walter Reed Project, Kisumu, Kenya.
FAU - Moro, Manuel
AU  - Moro M
AD  - National Institute of Health, Bethesda, Maryland, United States of America.
FAU - Gilman, Robert H
AU  - Gilman RH
AD  - Johns Hopkins University, Baltimore, Maryland, United States of America.
FAU - Harris, Nick
AU  - Harris N
AD  - ID-FISH Technology Inc., Palo Alto, California, United States of America; IGeneX 
      Inc. Palo Alto, California, United States of America.
LA  - eng
GR  - A1056785/PHS HHS/United States
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20150902
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Child
MH  - Child, Preschool
MH  - Hematologic Tests/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant
MH  - Malaria/*blood/*diagnosis
MH  - Middle Aged
MH  - Plasmodium/*isolation & purification
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Young Adult
PMC - PMC4558036
COIS- Competing Interests: JS, OM, HW, DW and NH are authors on patents listed below. 
      JS and NH have stocks in ID-FISH andIGeneX. HW and DW were employees and OM is an 
      employee of IGeneX and ID-FISH. They do not have any stock in these companies. AR 
      and SK are employees of and have financial interest in Nova Meditech Pvt. Ltd. 
      This study was supported by NIH SBIR Phase I and Phase II Grants # A1056785. 1. 
      NUCLEIC ACID PROBES AND METHODS FOR DETECTING PLASMODIUM PARASITES Nick S. 
      Harris, Monterey, California (United States); Suzanne Scherini-Ward, Santa Clara, 
      California (United States); Olivia Mark, San Jose, California (United States); 
      Jyotsna S. Shah, Santa Clara, California (United States); Helena Weltman Los 
      Altos, California (United States); Danuta Wronska Raleigh, North Carolina (United 
      States). Patent Number: US 007,927,801; Issue Date: 4/19/2011. 2. NUCLEIC ACID 
      PROBES AND METHODS FOR DETECTING PLASMODIUM PARASITES (54) (75) Inventors: 
      Jyotsna S. Shah, Santa Clara, California (United States); Danuta Wronska, 
      Raleigh, North Carolina (United States); Helena Weltman, Los Altos, California 
      (United States); Nick S. Harris, Monterey, California (United States); Suzanne 
      Scherini-Ward, Santa Clara, California (United States); Oliva Mark, San Jose, 
      California. Patent Number: US 008,323,901; Issue Date: 12/4/2012. 3. NUCLEIC ACID 
      PROBES AND METHODS FOR DETECTING PLASMODIUM PARASITES (54) (75) Inventors: 
      Jyotsna S. Shah, Santa Clara, California (United States); Danuta Wronska, 
      Raleigh, North Carolina (United States); Helena Weltman, Los Altos, California 
      (United States); Nick S. Harris, Monterey, California (United States); Suzanne 
      Scherini-Ward, Santa Clara, California (United States); Oliva Mark, San Jose, 
      California. Patent Number: US 008592568B2; Issue Date: 11/26/2013. The authors 
      confirm that this does not alter their adherence to all PLOS ONE policies on 
      sharing data and materials, as detailed online in our guide for authors 
      http://www.PLOSone.org/static/editorial.action#competing.
EDAT- 2015/09/04 06:00
MHDA- 2016/05/24 06:00
PMCR- 2015/09/02
CRDT- 2015/09/03 06:00
PHST- 2014/12/26 00:00 [received]
PHST- 2015/08/08 00:00 [accepted]
PHST- 2015/09/03 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2016/05/24 06:00 [medline]
PHST- 2015/09/02 00:00 [pmc-release]
AID - PONE-D-14-53523 [pii]
AID - 10.1371/journal.pone.0136726 [doi]
PST - epublish
SO  - PLoS One. 2015 Sep 2;10(9):e0136726. doi: 10.1371/journal.pone.0136726. 
      eCollection 2015.