PMID- 26334306 OWN - NLM STAT- MEDLINE DCOM- 20160404 LR - 20201215 IS - 1553-7374 (Electronic) IS - 1553-7366 (Print) IS - 1553-7366 (Linking) VI - 11 IP - 9 DP - 2015 Sep TI - Distinct but Spatially Overlapping Intestinal Niches for Vancomycin-Resistant Enterococcus faecium and Carbapenem-Resistant Klebsiella pneumoniae. PG - e1005132 LID - 10.1371/journal.ppat.1005132 [doi] LID - e1005132 AB - Antibiotic resistance among enterococci and gamma-proteobacteria is an increasing problem in healthcare settings. Dense colonization of the gut by antibiotic-resistant bacteria facilitates their spread between patients and also leads to bloodstream and other systemic infections. Antibiotic-mediated destruction of the intestinal microbiota and consequent loss of colonization resistance are critical factors leading to persistence and spread of antibiotic-resistant bacteria. The mechanisms underlying microbiota-mediated colonization resistance remain incompletely defined and are likely distinct for different antibiotic-resistant bacterial species. It is unclear whether enterococci or gamma-proteobacteria, upon expanding to high density in the gut, confer colonization resistance against competing bacterial species. Herein, we demonstrate that dense intestinal colonization with vancomycin-resistant Enterococcus faecium (VRE) does not reduce in vivo growth of carbapenem-resistant Klebsiella pneumoniae. Reciprocally, K. pneumoniae does not impair intestinal colonization by VRE. In contrast, transplantation of a diverse fecal microbiota eliminates both VRE and K. pneumoniae from the gut. Fluorescence in situ hybridization demonstrates that VRE and K. pneumoniae localize to the same regions in the colon but differ with respect to stimulation and invasion of the colonic mucus layer. While VRE and K. pneumoniae occupy the same three-dimensional space within the gut lumen, their independent growth and persistence in the gut suggests that they reside in distinct niches that satisfy their specific in vivo metabolic needs. FAU - Caballero, Silvia AU - Caballero S AD - Immunology Program and Infectious Disease Service, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America; Immunology and Microbial Pathogenesis Program, Weill Cornell Graduate School of Medical Sciences, New York, New York, United States of America. FAU - Carter, Rebecca AU - Carter R AD - Immunology Program and Infectious Disease Service, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America. FAU - Ke, Xu AU - Ke X AD - Molecular Cytology Core Facility, Sloan-Kettering Institute, New York, New York, United States of America. FAU - Susac, Boze AU - Susac B AD - Immunology Program and Infectious Disease Service, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America. FAU - Leiner, Ingrid M AU - Leiner IM AD - Immunology Program and Infectious Disease Service, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America. FAU - Kim, Grace J AU - Kim GJ AD - Lucille Castori Center for Microbes, Inflammation and Cancer, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America. FAU - Miller, Liza AU - Miller L AD - Lucille Castori Center for Microbes, Inflammation and Cancer, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America. FAU - Ling, Lilan AU - Ling L AD - Lucille Castori Center for Microbes, Inflammation and Cancer, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America. FAU - Manova, Katia AU - Manova K AD - Molecular Cytology Core Facility, Sloan-Kettering Institute, New York, New York, United States of America. FAU - Pamer, Eric G AU - Pamer EG AD - Immunology Program and Infectious Disease Service, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America; Immunology and Microbial Pathogenesis Program, Weill Cornell Graduate School of Medical Sciences, New York, New York, United States of America; Lucille Castori Center for Microbes, Inflammation and Cancer, Memorial Sloan-Kettering Cancer Center, New York, New York, United States of America. LA - eng GR - R01 AI42135/AI/NIAID NIH HHS/United States GR - HHMI/Howard Hughes Medical Institute/United States GR - R01 AI042135/AI/NIAID NIH HHS/United States GR - P30 CA008748/CA/NCI NIH HHS/United States GR - AI95706/AI/NIAID NIH HHS/United States GR - R01 AI095706/AI/NIAID NIH HHS/United States PT - Comparative Study PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't DEP - 20150903 PL - United States TA - PLoS Pathog JT - PLoS pathogens JID - 101238921 RN - 0 (Anti-Bacterial Agents) RN - 0 (Carbapenems) RN - 7C782967RD (Ampicillin) SB - IM MH - Ampicillin/adverse effects MH - Animals MH - Anti-Bacterial Agents/adverse effects/pharmacology MH - Carbapenems/pharmacology MH - Colony Count, Microbial MH - Drug Resistance, Bacterial MH - Enteritis/*microbiology/pathology/prevention & control MH - Enterococcus faecium/drug effects/growth & development/isolation & purification/*physiology MH - Fecal Microbiota Transplantation MH - Feces/microbiology MH - Female MH - Gastrointestinal Microbiome/drug effects MH - Gram-Positive Bacterial Infections/*microbiology/pathology/prevention & control MH - Host-Pathogen Interactions MH - In Situ Hybridization, Fluorescence MH - Intestinal Mucosa/drug effects/*microbiology/pathology MH - Klebsiella Infections/*microbiology/pathology/prevention & control MH - Klebsiella pneumoniae/drug effects/growth & development/isolation & purification/*physiology MH - Mice, Inbred C57BL MH - Microbial Interactions MH - Specific Pathogen-Free Organisms MH - Vancomycin-Resistant Enterococci/drug effects/growth & development/isolation & purification/*physiology PMC - PMC4559429 COIS- The authors have declared that no competing interests exist. EDAT- 2015/09/04 06:00 MHDA- 2016/04/05 06:00 PMCR- 2015/09/03 CRDT- 2015/09/04 06:00 PHST- 2015/05/19 00:00 [received] PHST- 2015/08/05 00:00 [accepted] PHST- 2015/09/04 06:00 [entrez] PHST- 2015/09/04 06:00 [pubmed] PHST- 2016/04/05 06:00 [medline] PHST- 2015/09/03 00:00 [pmc-release] AID - PPATHOGENS-D-15-01166 [pii] AID - 10.1371/journal.ppat.1005132 [doi] PST - epublish SO - PLoS Pathog. 2015 Sep 3;11(9):e1005132. doi: 10.1371/journal.ppat.1005132. eCollection 2015 Sep.