PMID- 26334937
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20220321
IS  - 1573-6776 (Electronic)
IS  - 0141-5492 (Linking)
VI  - 38
IP  - 1
DP  - 2016 Jan
TI  - Pretreatments with injured microenvironmental signals altered the characteristics 
      of human umbilical cord mesenchymal stem cells.
PG  - 157-65
LID - 10.1007/s10529-015-1946-6 [doi]
AB  - OBJECTIVE: Human umbilical cord mesenchymal stem cells (hUCMSCs) have 
      renoprotective effects but the influence of the microenvironment on 
      characteristics of hUCMSCs has not been well studied. Here, we investigate the 
      effects of injury conditions on properties of hUCMSCs. RESULTS: hUCMSCs were 
      treated in vitro under conditions mimicking the injury microenvironment of acute 
      kidney injury. Cells stimulated with factor-treated medium proliferated slowly at 
      first but quickly afterwards their morphology subsequently changed from spindle 
      to stellate shape. Increased number of cells with strong expression of thymine-1 
      (Thy-1) or alpha-smooth muscle actin (alpha-SMA) was detected at 1 or 2 weeks after 
      stimulation. Hepatocyte growth factor (HGF) level markedly increased after 
      culture for 6 h under hypoxia condition. The expressions of HGF and insulin 
      growth factor-1 (IGF-1) were significantly up-regulated from 0.22 +/- 0.03 to 0.9 +/- 
      0.02 and 0.07 +/- 0.03 to 0.19 +/- 0.01 in H/R-treated hUCMSCs respectively. 
      Co-culture with injured renal tubular epithelial cells significantly promoted the 
      expression of HGF (1.19 +/- 0.21) and IGF-1 (0.24 +/- 0.03) in hUCMSCs. CONCLUSION: 
      The characteristics of hUCMSCs change in response to inured conditions, which may 
      enhance the efficacy of stem cell therapy and provide novel strategies in 
      maximizing biological and functional properties of hUCMSCs.
FAU - Cao, Huiling
AU  - Cao H
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nanjing University of 
      Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, 
      Nanjing, Jiangsu, 210029, People's Republic of China.
FAU - Hui, Qian
AU  - Hui Q
AD  - Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of 
      Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of 
      China.
FAU - Yan, Yongmin
AU  - Yan Y
AD  - Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of 
      Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of 
      China.
FAU - Zhang, Chunbing
AU  - Zhang C
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nanjing University of 
      Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, 
      Nanjing, Jiangsu, 210029, People's Republic of China.
FAU - Yang, Xuewen
AU  - Yang X
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nanjing University of 
      Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, 
      Nanjing, Jiangsu, 210029, People's Republic of China.
FAU - Ge, Liang
AU  - Ge L
AD  - Department of Laboratory Medicine, Affiliated Hospital of Nanjing University of 
      Chinese Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, 
      Nanjing, Jiangsu, 210029, People's Republic of China.
FAU - Xu, Wenrong
AU  - Xu W
AD  - Jiangsu Key Laboratory of Medical Science and Laboratory Medicine, School of 
      Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, People's Republic of 
      China. iclsxu@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150903
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (ACTA2 protein, human)
RN  - 0 (Actins)
RN  - 0 (Culture Media)
RN  - 0 (HGF protein, human)
RN  - 0 (IGF1 protein, human)
RN  - 0 (Thy-1 Antigens)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Actins/metabolism
MH  - Acute Kidney Injury/therapy
MH  - Animals
MH  - Cell Hypoxia
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Culture Media/*pharmacology
MH  - Epithelial Cells/*cytology
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Hepatocyte Growth Factor/metabolism
MH  - Humans
MH  - In Vitro Techniques
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Kidney Tubules/*cytology
MH  - Mesenchymal Stem Cells/cytology/*drug effects
MH  - Pregnancy
MH  - Rats
MH  - Thy-1 Antigens/metabolism
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Hepatocyte growth factor
OT  - Insulin growth factor
OT  - Kidney injury
OT  - Mesenchymal stem cells
OT  - Pretreatment
OT  - Stem cell therapy
OT  - Umbilical cord stem cells
EDAT- 2015/09/04 06:00
MHDA- 2016/10/14 06:00
CRDT- 2015/09/04 06:00
PHST- 2015/06/23 00:00 [received]
PHST- 2015/08/26 00:00 [accepted]
PHST- 2015/09/04 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
AID - 10.1007/s10529-015-1946-6 [pii]
AID - 10.1007/s10529-015-1946-6 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2016 Jan;38(1):157-65. doi: 10.1007/s10529-015-1946-6. Epub 2015 
      Sep 3.