PMID- 26336584
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150904
LR  - 20240109
IS  - 1976-9148 (Print)
IS  - 2005-4483 (Electronic)
IS  - 1976-9148 (Linking)
VI  - 23
IP  - 5
DP  - 2015 Sep
TI  - Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in 
      vitro and in vivo.
PG  - 442-8
LID - 10.4062/biomolther.2015.045 [doi]
AB  - We evaluated the chondroprotective effects of wogonin by investigating its 
      effects on the gene expression and production of matrix metalloproteinase-3 
      (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on 
      production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured 
      in a monolayer, and RT-PCR was used to measure interleukin-1beta (IL-1beta)-induced 
      expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with 
      thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular 
      chondrocytes, the effects of wogonin on IL-1beta-induced production and proteolytic 
      activity of MMP-3 were investigated using western blot analysis and casein 
      zymography, respectively. The effect of wogonin on MMP-3 protein production was 
      also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the 
      expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of 
      type II collagen. Furthermore, wogonin inhibited the production and proteolytic 
      activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. 
      These results suggest that wogonin can regulate the gene expression and 
      production of MMP-3, by directly acting on articular chondrocytes.
FAU - Park, Jin Sung
AU  - Park JS
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
FAU - Lee, Hyun Jae
AU  - Lee HJ
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 301-131, Republic of Korea.
FAU - Lee, Dong Yeong
AU  - Lee DY
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
FAU - Jo, Ho Seung
AU  - Jo HS
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
FAU - Jeong, Jin Hoon
AU  - Jeong JH
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
FAU - Kim, Dong Hee
AU  - Kim DH
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
FAU - Nam, Dae Cheol
AU  - Nam DC
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
FAU - Lee, Choong Jae
AU  - Lee CJ
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 301-131, Republic of Korea.
FAU - Hwang, Sun-Chul
AU  - Hwang SC
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 660-701, Republic of 
      Korea.
LA  - eng
PT  - Journal Article
DEP - 20150901
PL  - Korea (South)
TA  - Biomol Ther (Seoul)
JT  - Biomolecules & therapeutics
JID - 101472832
PMC - PMC4556204
OTO - NOTNLM
OT  - Chondrocyte
OT  - Metalloproteinase
OT  - Osteoarthritis
OT  - Wogonin
EDAT- 2015/09/04 06:00
MHDA- 2015/09/04 06:01
PMCR- 2015/09/01
CRDT- 2015/09/04 06:00
PHST- 2015/04/23 00:00 [received]
PHST- 2015/05/26 00:00 [revised]
PHST- 2015/06/09 00:00 [accepted]
PHST- 2015/09/04 06:00 [entrez]
PHST- 2015/09/04 06:00 [pubmed]
PHST- 2015/09/04 06:01 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - bt-23-442 [pii]
AID - 10.4062/biomolther.2015.045 [doi]
PST - ppublish
SO  - Biomol Ther (Seoul). 2015 Sep;23(5):442-8. doi: 10.4062/biomolther.2015.045. Epub 
      2015 Sep 1.