PMID- 26337432
OWN - NLM
STAT- MEDLINE
DCOM- 20160726
LR  - 20181202
IS  - 1435-4373 (Electronic)
IS  - 0934-9723 (Linking)
VI  - 34
IP  - 11
DP  - 2015 Nov
TI  - Rapid detection of carbapenemase activity: benefits and weaknesses of MALDI-TOF 
      MS.
PG  - 2225-34
LID - 10.1007/s10096-015-2473-z [doi]
AB  - Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass 
      spectrometry (MS) has been introduced as an identification procedure for bacteria 
      and fungi. The MALDI-TOF MS-based analysis of resistance to beta-lactam antibiotics 
      has been applied to detect hydrolysis of carbapenems by different bacterial 
      strains. However, the detection of enzymatic carbapenem degradation by MALDI-TOF 
      MS lacks well-standardized protocols and several methods and models of 
      interpretation using different calculations of ratio-of-peak intensities have 
      been described in the literature. Here, we used faropenem and ertapenem 
      hydrolysis as model compounds. In an attempt to propose a universal protocol, the 
      hydrolysis was regularly monitored during 24 h using well-characterized bacterial 
      strains producing different types of carbapenemases (KPC, IMP, NDM, VIM, and 
      OXA-48). Variable responses and different timing for detectable hydrolysis, 
      depending on the enzyme produced, were observed. KPC degrades its template 
      antibiotics very quickly (15 min for some KPC producers) compared to other types 
      of enzymes (more than 90 min for other enzymes). Prior bacterial lysis was shown 
      to be of no interest in the modulation or optimization of the hydrolytic 
      kinetics. The adequate detection of carbapenem hydrolysis would, therefore, 
      require several MALDI-TOF MS readouts for the timely detection of rapid 
      hydrolysis without missing slow hydrolysis. This enzymatic constraint limits the 
      implementation of a standard protocol in routine microbiology laboratories.
FAU - Mirande, C
AU  - Mirande C
AD  - Microbiology Innovation Unit, bioMerieux SA, 3 route de Port Michaud, La 
      Balme-les-Grottes, France. caroline.mirande@biomerieux.com.
FAU - Canard, I
AU  - Canard I
AD  - Microbiology Innovation Unit, bioMerieux SA, 3 route de Port Michaud, La 
      Balme-les-Grottes, France.
FAU - Buffet Croix Blanche, S
AU  - Buffet Croix Blanche S
AD  - Microbiology Innovation Unit, bioMerieux SA, 3 route de Port Michaud, La 
      Balme-les-Grottes, France.
FAU - Charrier, J-P
AU  - Charrier JP
AD  - TRD-Innovation Unit, bioMerieux SA, Marcy L'Etoile, France.
FAU - van Belkum, A
AU  - van Belkum A
AD  - R&D Microbiology, bioMerieux SA, La Balme-les-Grottes, France.
FAU - Welker, M
AU  - Welker M
AD  - R&D Microbiology, bioMerieux SA, La Balme-les-Grottes, France.
FAU - Chatellier, S
AU  - Chatellier S
AD  - Microbiology Innovation Unit, bioMerieux SA, 3 route de Port Michaud, La 
      Balme-les-Grottes, France.
LA  - eng
PT  - Journal Article
DEP - 20150904
PL  - Germany
TA  - Eur J Clin Microbiol Infect Dis
JT  - European journal of clinical microbiology & infectious diseases : official 
      publication of the European Society of Clinical Microbiology
JID - 8804297
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Proteins)
RN  - 0 (beta-Lactams)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - EC 3.5.2.6 (carbapenemase)
RN  - F52Y83BGH3 (fropenem)
RN  - G32F6EID2H (Ertapenem)
SB  - IM
MH  - Anti-Bacterial Agents/*metabolism
MH  - Bacterial Proteins/*analysis
MH  - Ertapenem
MH  - Fungi
MH  - Humans
MH  - Hydrolysis
MH  - Kinetics
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods
MH  - Time Factors
MH  - beta-Lactamases/*analysis
MH  - beta-Lactams/metabolism
EDAT- 2015/09/05 06:00
MHDA- 2016/07/28 06:00
CRDT- 2015/09/05 06:00
PHST- 2015/08/11 00:00 [received]
PHST- 2015/08/16 00:00 [accepted]
PHST- 2015/09/05 06:00 [entrez]
PHST- 2015/09/05 06:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
AID - 10.1007/s10096-015-2473-z [pii]
AID - 10.1007/s10096-015-2473-z [doi]
PST - ppublish
SO  - Eur J Clin Microbiol Infect Dis. 2015 Nov;34(11):2225-34. doi: 
      10.1007/s10096-015-2473-z. Epub 2015 Sep 4.