PMID- 26337896
OWN - NLM
STAT- MEDLINE
DCOM- 20160907
LR  - 20151127
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 99
IP  - 24
DP  - 2015 Dec
TI  - Sugarcane bagasse as support for immobilization of Bacillus pumilus HZ-2 and its 
      use in bioremediation of mesotrione-contaminated soils.
PG  - 10839-51
LID - 10.1007/s00253-015-6935-0 [doi]
AB  - The degrading microorganisms isolated from environment usually fail to degrade 
      pollutants when used for bioremediation of contaminated soils; thus, additional 
      treatments are needed to enhance biodegradation. In the present study, the 
      potential of sugarcane bagasse as bacteria-immobilizing support was investigated 
      in mesotrione biodegradation. A novel isolate Bacillus pumilus HZ-2 was applied 
      in bacterial immobilization, which was capable of degrading over 95 % of 
      mesotrione at initial concentrations ranging from 25 to 200 mg L(-1) within 
      4 days in flask-shaking tests. Scanning electron microscope (SEM) images showed 
      that the bacterial cells were strongly absorbed and fully dispersed on bagasse 
      surface after immobilization. Specially, 86.5 and 82.9 % of mesotrione was 
      eliminated by bacteria immobilized on bagasse of 100 and 60 mesh, respectively, 
      which indicated that this immobilization was able to maintain a high degrading 
      activity of the bacteria. Analysis of the degradation products determined 
      2-amino-4-methylsulfonylbenzoic acid (AMBA) and 4-methylsulfonyl-2-nitrobenzoic 
      acid (MNBA) as the main metabolites in the biodegradation pathway of mesotrione. 
      In the sterile soil, approximately 90 % of mesotrione was degraded after 
      supplementing 5.0 % of molasses in bacteria-bagasse composite, which greatly 
      enhanced microbial adaptability and growth in the soil environment. In the field 
      tests, over 75 % of mesotrione in soil was degraded within 14 days. The 
      immobilized preparation demonstrated that mesotrione could be degraded at a wide 
      range of pH values (5.0-8.0) and temperatures (25-35  degrees C), especially at low 
      concentrations of mesotrione (5 to 20 mg kg(-1)). These results showed that 
      sugarcane bagasse might be a good candidate as bacteria-immobilizing support to 
      enhance mesotrione degradation by Bacillus p. HZ-2 in contaminated soils.
FAU - Liu, Jie
AU  - Liu J
AD  - Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, 
      and Lab of Insect Toxicology, South China Agricultural University, Guangzhou, 
      510642, People's Republic of China.
FAU - Chen, Shaohua
AU  - Chen S
AD  - Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South 
      China Agricultural University, Guangzhou, 510642, People's Republic of China.
FAU - Ding, Jie
AU  - Ding J
AD  - Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, 
      and Lab of Insect Toxicology, South China Agricultural University, Guangzhou, 
      510642, People's Republic of China.
FAU - Xiao, Ying
AU  - Xiao Y
AD  - Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, 
      and Lab of Insect Toxicology, South China Agricultural University, Guangzhou, 
      510642, People's Republic of China.
FAU - Han, Haitao
AU  - Han H
AD  - Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, 
      and Lab of Insect Toxicology, South China Agricultural University, Guangzhou, 
      510642, People's Republic of China.
FAU - Zhong, Guohua
AU  - Zhong G
AD  - Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, 
      and Lab of Insect Toxicology, South China Agricultural University, Guangzhou, 
      510642, People's Republic of China. guohuazhong@scau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150904
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Cyclohexanones)
RN  - 0 (Soil Pollutants)
RN  - 48TR68G21T (mesotrione)
RN  - 9004-34-6 (Cellulose)
RN  - 9006-97-7 (bagasse)
SB  - IM
MH  - Bacillus/metabolism/*physiology
MH  - *Bacterial Adhesion
MH  - *Biodegradation, Environmental
MH  - Cellulose/*metabolism
MH  - Cyclohexanones/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Microscopy, Electron, Scanning
MH  - Saccharum/*metabolism
MH  - Soil Microbiology
MH  - Soil Pollutants/*metabolism
MH  - Temperature
OTO - NOTNLM
OT  - Bioremediation
OT  - Immobilization
OT  - Mesotrione
OT  - Metabolites
OT  - Sugarcane bagasse
EDAT- 2015/09/05 06:00
MHDA- 2016/09/08 06:00
CRDT- 2015/09/05 06:00
PHST- 2015/05/01 00:00 [received]
PHST- 2015/08/11 00:00 [accepted]
PHST- 2015/07/21 00:00 [revised]
PHST- 2015/09/05 06:00 [entrez]
PHST- 2015/09/05 06:00 [pubmed]
PHST- 2016/09/08 06:00 [medline]
AID - 10.1007/s00253-015-6935-0 [pii]
AID - 10.1007/s00253-015-6935-0 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2015 Dec;99(24):10839-51. doi: 
      10.1007/s00253-015-6935-0. Epub 2015 Sep 4.