PMID- 26339395
OWN - NLM
STAT- MEDLINE
DCOM- 20160607
LR  - 20181113
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 8
IP  - 7
DP  - 2015
TI  - Cerebellar neuronal apoptosis in heroin-addicted rats and its molecular 
      mechanism.
PG  - 8260-7
AB  - BACKGROUND: The overall objective of this study was to investigate neuronal 
      apoptosis and expression of apoptosis related proteins (c-jun, cytc and Bax) in 
      the cerebellum of rates with heroin addiction. MATERIAL/METHODS: 40 adult male 
      Sprague-Dawley rats which weighing 200-220 g were randomly divided into 5 groups 
      (n = 8 per group): control group, 10-day heroin-addicted group, 20-day 
      heroin-addicted group, 30-day heroin-addicted group and 40-day heroin-addicted 
      group. Rats in the control group were treated with normal saline. Rats in the 
      addiction groups (20 d, 30 d, 40 d) were all given subcutaneous injection with 
      heroin for 15 days to induce heroin addiction. After injected with heroin for 15 
      days, rats were treated with naloxone at a dose of 5 mg/kg to induce abstinence 
      for 30 mins to examine the addiction of rats. They were then continued to be 
      treated with heroin for another 10 days, 20 days, 30 days, and 40 days 
      respectively to establish heroin-addicted models. Terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) was employed to identify 
      apoptotic cells [6]. Immunohistochemistry and Western blot assay were also used 
      in the study to examine the protein expressions of c-jun, cytc and Bax in the 
      cerebellum. RESULTS: Compared with the control group, the proportion of apoptotic 
      neurons increased significantly in the heroin addiction groups (10 d, 20 d, 30 d, 
      40 d) (P < 0.05), also accompanied by markedly increased expressions of c-jun, 
      cytc and Bax (P < 0.05) depending on doses of heroin in the cerebellum. Thus, the 
      significant differences were observed in heroin addiction groups (10 d, 20 d,30 
      d, 40 d) and control group (P < 0.05). CONCLUSION: Long-term use of heroin may 
      induce neuronal apoptosis in the cerebellum by raising the expressions of 
      pro-apoptotic c-jun, cytc and Bax, which might be one of mechanisms underlying 
      the heroin-induced cerebellum neuronal damage.
FAU - Pu, Hongwei
AU  - Pu H
AD  - Department of Science and Research Education Center, First Affiliated Hospital of 
      Xinjiang Medical University Urumqi, China.
FAU - Wang, Xuemei
AU  - Wang X
AD  - Xinjiang Key Laboratory of Medical animal Model Research, First Affiliated 
      Hospital of Xinjiang Medical University Urumqi, China.
FAU - Zhang, Jianlong
AU  - Zhang J
AD  - College of Basic Medicine, Xinjiang Medical University Urumqi, China.
FAU - Ma, Chuang
AU  - Ma C
AD  - Department of Micro-Reconstructive Surgery of Orthopedics Center, First 
      Affiliated Hospital of Xinjiang Medical University Urumqi, China.
FAU - Su, Yinxia
AU  - Su Y
AD  - Clinical Research Institute of The First Affiliated Hospital of Xinjiang Medical 
      University Urumqi, China.
FAU - Li, Xiujuan
AU  - Li X
AD  - College of Basic Medicine, Xinjiang Medical University Urumqi, China.
FAU - Liu, Xiaoshan
AU  - Liu X
AD  - Department of Forensic Science, Zhongshan School of Medicine, Sun Yat-Sen 
      University Guangzhou, China.
FAU - Su, Liping
AU  - Su L
AD  - Department of Pathology, First Affiliated Hospital of Xinjiang Medical University 
      Urumqi, China.
LA  - eng
PT  - Journal Article
DEP - 20150701
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (BAX protein, human)
RN  - 0 (Biomarkers)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 9007-43-6 (Cytochromes c)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Biomarkers/metabolism
MH  - Blotting, Western
MH  - Cerebellum/metabolism/*pathology
MH  - Cytochromes c/metabolism
MH  - Disease Models, Animal
MH  - Heroin Dependence/metabolism/*pathology
MH  - Immunohistochemistry
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Neurons/metabolism/*pathology
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - bcl-2-Associated X Protein/metabolism
PMC - PMC4555723
OTO - NOTNLM
OT  - Bax
OT  - Heroin addiction
OT  - apoptosis
OT  - c-jun
OT  - cerebellum
OT  - cytc
EDAT- 2015/09/05 06:00
MHDA- 2016/06/09 06:00
PMCR- 2015/07/01
CRDT- 2015/09/05 06:00
PHST- 2015/05/06 00:00 [received]
PHST- 2015/06/23 00:00 [accepted]
PHST- 2015/09/05 06:00 [entrez]
PHST- 2015/09/05 06:00 [pubmed]
PHST- 2016/06/09 06:00 [medline]
PHST- 2015/07/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2015 Jul 1;8(7):8260-7. eCollection 2015.