PMID- 26340061
OWN - NLM
STAT- MEDLINE
DCOM- 20160726
LR  - 20221207
IS  - 1879-2448 (Electronic)
IS  - 0043-1354 (Linking)
VI  - 85
DP  - 2015 Nov 15
TI  - Improvement of the trace metal composition of medium for nitrite-dependent 
      anaerobic methane oxidation bacteria: Iron (II) and copper (II) make a 
      difference.
PG  - 235-43
LID - S0043-1354(15)30191-3 [pii]
LID - 10.1016/j.watres.2015.08.040 [doi]
AB  - Nitrite-dependent anaerobic methane oxidation (n-damo) is a potential bioprocess 
      for treating nitrogen-containing wastewater. This process uses methane, an 
      inexpensive and nontoxic end-product of anaerobic digestion, as an external 
      electron donor. However, the low turnover rate and slow growth rate of n-damo 
      functional bacteria limit the practical application of this process. In the 
      present study, the short- and long-term effects of variations in trace metal 
      concentrations on n-damo bacteria were investigated, and the concentrations of 
      trace metal elements of medium were improved. The results were subsequently 
      verified by a group of long-term inoculations (90 days) and were applied in a 
      sequencing batch reactor (SBR) (84 days). The results indicated that iron 
      (Fe(II)) and copper (Cu(II)) (20 and 10 mumol L(-1), respectively) significantly 
      stimulated the activity and the growth of n-damo bacteria, whereas other trace 
      metal elements, including zinc (Zn), molybdenum (Mo), cobalt (Co), manganese 
      (Mn), and nickel (Ni), had no significant effect on n-damo bacteria in the tested 
      concentration ranges. Interestingly, fluorescence in situ hybridization (FISH) 
      showed that a large number of dense, large aggregates (10-50 mum) of n-damo 
      bacteria were formed by cell adhesion in the SBR reactor after using the improved 
      medium, and to our knowledge this is the first discovery of large aggregates of 
      n-damo bacteria.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - He, Zhanfei
AU  - He Z
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - Geng, Sha
AU  - Geng S
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - Pan, Yawei
AU  - Pan Y
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - Cai, Chaoyang
AU  - Cai C
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - Wang, Jiaqi
AU  - Wang J
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - Wang, Liqiao
AU  - Wang L
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - Liu, Shuai
AU  - Liu S
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - Zheng, Ping
AU  - Zheng P
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - Xu, Xinhua
AU  - Xu X
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China.
FAU - Hu, Baolan
AU  - Hu B
AD  - Department of Environmental Engineering, Zhejiang University, Hangzhou, 310058, 
      China. Electronic address: blhu@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150824
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (Metals)
RN  - 0 (Waste Water)
RN  - 789U1901C5 (Copper)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Anaerobiosis
MH  - Bioreactors
MH  - Copper/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Iron/*metabolism
MH  - Metals/*metabolism
MH  - Methylococcaceae/*metabolism
MH  - Waste Disposal, Fluid/*methods
MH  - Wastewater
OTO - NOTNLM
OT  - Biological nitrogen removal
OT  - Medium improvement
OT  - Nitrite-dependent anaerobic methane oxidation (n-damo)
OT  - Transition metal
EDAT- 2015/09/05 06:00
MHDA- 2016/07/28 06:00
CRDT- 2015/09/05 06:00
PHST- 2015/04/24 00:00 [received]
PHST- 2015/08/18 00:00 [revised]
PHST- 2015/08/22 00:00 [accepted]
PHST- 2015/09/05 06:00 [entrez]
PHST- 2015/09/05 06:00 [pubmed]
PHST- 2016/07/28 06:00 [medline]
AID - S0043-1354(15)30191-3 [pii]
AID - 10.1016/j.watres.2015.08.040 [doi]
PST - ppublish
SO  - Water Res. 2015 Nov 15;85:235-43. doi: 10.1016/j.watres.2015.08.040. Epub 2015 
      Aug 24.