PMID- 26342308
OWN - NLM
STAT- MEDLINE
DCOM- 20161025
LR  - 20191210
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 408
IP  - 7
DP  - 2016 Mar
TI  - Molecularly imprinted polymer beads for clean-up and preconcentration of 
      beta-lactamase-resistant penicillins in milk.
PG  - 1843-54
LID - 10.1007/s00216-015-8941-6 [doi]
AB  - This work describes the development and application of class-selective 
      molecularly imprinted polymers (MIPs) for the analysis of 
      beta-lactamase-resistant penicillins, namely cloxacillin (CLOXA), oxacillin 
      (OXA), and dicloxacillin (DICLOXA), in milk samples. Our method is based on 
      molecularly imprinted solid-phase extraction (MISPE) coupled to high-performance 
      liquid chromatography (HPLC) with diode-array detection (DAD). 
      2-Biphenylylpenicillin (2BPEN), a surrogate with a close resemblance to 
      beta-lactamase-resistant penicillins in terms of size, shape, hydrophobicity, and 
      functionality, was synthesized and used as the template for the polymer 
      synthesis. A MIP library was prepared and screened to select the optimum 
      functional monomer, N-(2-aminoethyl)methacrylamide, and cross-linker, 
      trimethylolpropane trimethacrylate, that provided the best recognition for the 
      target antibiotics. For the MISPE application, the MIPs were prepared in the form 
      of microspheres, using porous silica beads (40-75 mum) as sacrificial scaffolds. 
      The developed MISPE method enables efficient extraction from aqueous samples and 
      analysis of the antimicrobials, when followed by a selective washing with 2 mL 
      acetonitrile-water (20:80 v/v) and elution with 1 mL 0.05 mol L(-1) 
      tetrabutylammonium in methanol. The analytical method was validated according to 
      EU guideline 2002/657/EC. The limits of quantification (S/N = 10) were in the 
      5.3-6.3 mug kg(-1) range, well below the maximum residue limits (MRLs) currently 
      established. Inter-day mean recoveries were in the range 99-102 % with RSDs below 
      9 %, improving on the performance of previously reported MISPE methods for the 
      analysis of CLOXA, OXA, or DICLOXA in milk samples.
FAU - Urraca, Javier L
AU  - Urraca JL
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense 
      de Madrid, 28040, Madrid, Spain. jurracar@quim.ucm.es.
AD  - CEI Campus Moncloa, UCM-UPM, Avda Complutense s/n, 28040, Madrid, Spain. 
      jurracar@quim.ucm.es.
FAU - Chamorro-Mendiluce, Raquel
AU  - Chamorro-Mendiluce R
AD  - Department of Organic Chemistry, Faculty of Chemistry, Universidad Complutense de 
      Madrid, 28040, Madrid, Spain.
FAU - Orellana, Guillermo
AU  - Orellana G
AD  - Department of Organic Chemistry, Faculty of Chemistry, Universidad Complutense de 
      Madrid, 28040, Madrid, Spain.
FAU - Moreno-Bondi, Maria C
AU  - Moreno-Bondi MC
AD  - Department of Analytical Chemistry, Faculty of Chemistry, Universidad Complutense 
      de Madrid, 28040, Madrid, Spain. mcmbondi@quim.ucm.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20150905
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Penicillins)
RN  - 0 (Polymers)
RN  - COF19H7WBK (Dicloxacillin)
RN  - EC 3.5.2.6 (beta-Lactamases)
RN  - O6X5QGC2VB (Cloxacillin)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*isolation & purification
MH  - Chromatography, High Pressure Liquid/methods
MH  - Cloxacillin/isolation & purification
MH  - Dicloxacillin/isolation & purification
MH  - Limit of Detection
MH  - Milk/*chemistry
MH  - Molecular Imprinting/*methods
MH  - Oxacillin/isolation & purification
MH  - Penicillins/*isolation & purification
MH  - Polymers/*chemistry
MH  - Solid Phase Extraction/*methods
MH  - beta-Lactamases/metabolism
OTO - NOTNLM
OT  - MISPE
OT  - Method validation
OT  - Milk
OT  - Molecularly imprinted polymers
OT  - Penicillin
OT  - Surrogate template
EDAT- 2015/09/08 06:00
MHDA- 2016/10/26 06:00
CRDT- 2015/09/07 06:00
PHST- 2015/06/07 00:00 [received]
PHST- 2015/07/25 00:00 [accepted]
PHST- 2015/07/21 00:00 [revised]
PHST- 2015/09/07 06:00 [entrez]
PHST- 2015/09/08 06:00 [pubmed]
PHST- 2016/10/26 06:00 [medline]
AID - 10.1007/s00216-015-8941-6 [pii]
AID - 10.1007/s00216-015-8941-6 [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2016 Mar;408(7):1843-54. doi: 10.1007/s00216-015-8941-6. Epub 
      2015 Sep 5.