PMID- 26342466
OWN - NLM
STAT- MEDLINE
DCOM- 20160202
LR  - 20151023
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 485
DP  - 2015 Nov
TI  - Chimeric porcine reproductive and respiratory syndrome virus containing shuffled 
      multiple envelope genes confers cross-protection in pigs.
PG  - 402-13
LID - S0042-6822(15)00378-5 [pii]
LID - 10.1016/j.virol.2015.08.021 [doi]
AB  - The extensive genetic diversity of porcine reproductive and respiratory syndrome 
      virus (PRRSV) strains is a major obstacle for vaccine development. We previously 
      demonstrated that chimeric PRRSVs in which a single envelope gene (ORF3, ORF4, 
      ORF5 or ORF6) was shuffled via DNA shuffling had an improved heterologous 
      cross-neutralizing ability. In this study, we incorporate all of the 
      individually-shuffled envelope genes together in different combinations into an 
      infectious clone backbone of PRRSV MLV Fostera((R)) PRRS. Five viable progeny 
      chimeric viruses were rescued, and their growth characteristics were 
      characterized in vitro. In a pilot pig study, two chimeric viruses 
      (FV-SPDS-VR2,FV-SPDS-VR5) were found to induce cross-neutralizing antibodies 
      against heterologous strains. A subsequent vaccination/challenge study in 72 pigs 
      revealed that chimeric virus FV-SPDS-VR2 and parental virus conferred partial 
      cross-protection when challenged with heterologous strains NADC20 or MN184B. The 
      results have important implications for future development of an effective PRRSV 
      vaccine that confers heterologous protection.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Tian, Debin
AU  - Tian D
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA.
FAU - Ni, Yan-Yan
AU  - Ni YY
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA.
FAU - Opriessnig, Tanja
AU  - Opriessnig T
AD  - The Roslin Institute and The Royal (Dick) School of Veterinary Studies, 
      University of Edinburgh, Midlothian, UK.
FAU - Cao, Dianjun
AU  - Cao D
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA.
FAU - Pineyro, Pablo
AU  - Pineyro P
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA; Department of Veterinary Diagnostic and Production Animal Medicine, Iowa 
      State University College of Veterinary Medicine, Ames, IA 5001, USA.
FAU - Yugo, Danielle M
AU  - Yugo DM
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA.
FAU - Overend, Christopher
AU  - Overend C
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA.
FAU - Cao, Qian
AU  - Cao Q
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA.
FAU - Lynn Heffron, C
AU  - Lynn Heffron C
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA.
FAU - Halbur, Patrick G
AU  - Halbur PG
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University College of Veterinary Medicine, Ames, IA 5001, USA.
FAU - Pearce, Douglas S
AU  - Pearce DS
AD  - Zoetis Inc., Kalamazoo, MI, USA.
FAU - Calvert, Jay G
AU  - Calvert JG
AD  - Zoetis Inc., Kalamazoo, MI, USA.
FAU - Meng, Xiang-Jin
AU  - Meng XJ
AD  - Department of Biomedical Sciences and Pathobiology, College of Veterinary 
      Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA, 
      USA. Electronic address: xjmeng@vt.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150902
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Neutralizing/immunology
MH  - Antibodies, Viral/immunology
MH  - Cell Line
MH  - Cross Protection/*immunology
MH  - Gene Order
MH  - Genome, Viral
MH  - Immunization
MH  - Neutralization Tests
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/pathology/prevention & 
      control/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*immunology
MH  - Reassortant Viruses
MH  - *Recombination, Genetic
MH  - Swine
MH  - Viral Envelope Proteins/*genetics/*immunology
MH  - Virus Replication
OTO - NOTNLM
OT  - Cross-protection
OT  - DNA shuffling
OT  - Envelope genes
OT  - Heterologous strains
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Vaccines
EDAT- 2015/09/08 06:00
MHDA- 2016/02/03 06:00
CRDT- 2015/09/07 06:00
PHST- 2015/06/18 00:00 [received]
PHST- 2015/08/14 00:00 [revised]
PHST- 2015/08/19 00:00 [accepted]
PHST- 2015/09/07 06:00 [entrez]
PHST- 2015/09/08 06:00 [pubmed]
PHST- 2016/02/03 06:00 [medline]
AID - S0042-6822(15)00378-5 [pii]
AID - 10.1016/j.virol.2015.08.021 [doi]
PST - ppublish
SO  - Virology. 2015 Nov;485:402-13. doi: 10.1016/j.virol.2015.08.021. Epub 2015 Sep 2.