PMID- 26343271
OWN - NLM
STAT- MEDLINE
DCOM- 20160125
LR  - 20221207
IS  - 1938-2650 (Electronic)
IS  - 0001-5547 (Linking)
VI  - 59
IP  - 4
DP  - 2015
TI  - Clinical Role of the Detection of Human Telomerase RNA Component Gene 
      Amplification by Fluorescence in situ Hybridization on Liquid-Based Cervical 
      Samples: Comparison with Human Papillomavirus-DNA Testing and Histopathology.
PG  - 345-54
LID - 10.1159/000438719 [doi]
AB  - OBJECTIVE: This study was designed to evaluate whether the adjunct of human 
      telomerase RNA component (hTERC) fluorescence in situ hybridization (FISH) 
      analysis to cytological diagnosis and human papillomavirus (HPV)-DNA testing may 
      serve as a predictive marker for distinguishing cervical lesions destined to 
      regress from those at high risk of progression towards invasive cancer. STUDY 
      DESIGN: hTERC FISH analysis was performed on 54 residual liquid-based cytology 
      specimens obtained from women referred to colposcopy for the detection of 
      atypical squamous cells of undetermined significance or worse (ASCUS+) lesions. 
      Histological diagnosis was considered the gold standard and cervical 
      intraepithelial neoplasia of grade 2 or worse (CIN2+) as the worst outcome. 
      RESULTS: Oncogenic HPV-DNA was found in 96.3% of the specimens. Among these, 
      38.5% revealed a CIN2+ diagnosis. hTERC gene amplification was detected in 37% of 
      the cases; among these, 70% showed up as CIN2+. hTERC FISH analysis significantly 
      improves the specificity and positive predictive value of HPV-DNA testing, thus 
      differentiating patients with a CIN2+ diagnosis from those with a CIN2- 
      diagnosis. CONCLUSIONS: Despite the limitation of a small study sample, our 
      findings provide promising data, indicating the possible role of hTERC analysis 
      in the assessment of the risk of developing cervical cancer. This approach would 
      implement the specificity of DNA testing, avoiding overtreatment at the same 
      time. Prospective follow-up studies are needed with the aim of introducing hTERC 
      FISH into decision-making algorithms.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Zappacosta, Roberta
AU  - Zappacosta R
AD  - Surgical Pathology Unit, x2018;SS Annunziata Hospital' of Chieti, Chieti Scalo, 
      Italy.
FAU - Ianieri, Manuel Maria
AU  - Ianieri MM
FAU - Buca, Danilo
AU  - Buca D
FAU - Repetti, Elena
AU  - Repetti E
FAU - Ricciardulli, Alessandra
AU  - Ricciardulli A
FAU - Liberati, Marco
AU  - Liberati M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20150908
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
RN  - 0 (DNA, Viral)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Colposcopy/methods
MH  - DNA, Viral/*genetics
MH  - Female
MH  - Gene Amplification
MH  - Human Papillomavirus DNA Tests/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Middle Aged
MH  - Papillomaviridae/genetics
MH  - Papillomavirus Infections/diagnosis/pathology/virology
MH  - RNA/*genetics
MH  - Telomerase/*genetics
MH  - Uterine Cervical Neoplasms/*genetics/*pathology/virology
MH  - Young Adult
MH  - Uterine Cervical Dysplasia/diagnosis/pathology/virology
EDAT- 2015/09/08 06:00
MHDA- 2016/01/26 06:00
CRDT- 2015/09/08 06:00
PHST- 2015/02/04 00:00 [received]
PHST- 2015/07/13 00:00 [accepted]
PHST- 2015/09/08 06:00 [entrez]
PHST- 2015/09/08 06:00 [pubmed]
PHST- 2016/01/26 06:00 [medline]
AID - 000438719 [pii]
AID - 10.1159/000438719 [doi]
PST - ppublish
SO  - Acta Cytol. 2015;59(4):345-54. doi: 10.1159/000438719. Epub 2015 Sep 8.