PMID- 26344907
OWN - NLM
STAT- MEDLINE
DCOM- 20160802
LR  - 20181113
IS  - 2213-6711 (Electronic)
IS  - 2213-6711 (Linking)
VI  - 5
IP  - 4
DP  - 2015 Oct 13
TI  - PTPN13 and beta-Catenin Regulate the Quiescence of Hematopoietic Stem Cells and 
      Their Interaction with the Bone Marrow Niche.
PG  - 516-31
LID - S2213-6711(15)00237-4 [pii]
LID - 10.1016/j.stemcr.2015.08.003 [doi]
AB  - The regulation of hematopoietic stem cells (HSCs) depends on the integration of 
      the multiple signals received from the bone marrow niche. We show the relevance 
      of the protein tyrosine phosphatase PTPN13 and beta-catenin as intracellular 
      signaling molecules to control HSCs adhesiveness, cell cycling, and quiescence. 
      Lethally irradiated mice transplanted with Lin(-) bone marrow cells in which 
      PTPN13 or beta-catenin had been silenced showed a significant increase of long-term 
      (LT) and short-term (ST) HSCs. A decrease in cycling cells was also found, 
      together with an increase in quiescence. The decreased expression of PTPN13 or 
      beta-catenin was linked to the upregulation of several genes coding for integrins 
      and several cadherins, explaining the higher cell adhesiveness. Our data are 
      consistent with the notion that the levels of PTPN13 and beta-catenin must be 
      strictly regulated by extracellular signaling to regulate HSC attachment to the 
      niche and the balance between proliferation and quiescence.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Lopez-Ruano, Guillermo
AU  - Lopez-Ruano G
AD  - Department of Biochemistry and Molecular Biology, University of Salamanca, 
      Salamanca 37007, Spain; IBSAL (Instituto de Investigacion Biomedica de 
      Salamanca), Salamanca 37007, Spain.
FAU - Prieto-Bermejo, Rodrigo
AU  - Prieto-Bermejo R
AD  - Department of Biochemistry and Molecular Biology, University of Salamanca, 
      Salamanca 37007, Spain; IBSAL (Instituto de Investigacion Biomedica de 
      Salamanca), Salamanca 37007, Spain.
FAU - Ramos, Teresa L
AU  - Ramos TL
AD  - IBSAL (Instituto de Investigacion Biomedica de Salamanca), Salamanca 37007, 
      Spain.
FAU - San-Segundo, Laura
AU  - San-Segundo L
AD  - IBSAL (Instituto de Investigacion Biomedica de Salamanca), Salamanca 37007, 
      Spain.
FAU - Sanchez-Abarca, Luis Ignacio
AU  - Sanchez-Abarca LI
AD  - Department of Hematology, Hospital Universitario Virgen del 
      Rocio/IBIS/CSIC/University of Seville, Seville 41013, Spain.
FAU - Sanchez-Guijo, Fermin
AU  - Sanchez-Guijo F
AD  - IBSAL (Instituto de Investigacion Biomedica de Salamanca), Salamanca 37007, 
      Spain.
FAU - Perez-Simon, Jose Antonio
AU  - Perez-Simon JA
AD  - Department of Hematology, Hospital Universitario Virgen del 
      Rocio/IBIS/CSIC/University of Seville, Seville 41013, Spain.
FAU - Sanchez-Yague, Jesus
AU  - Sanchez-Yague J
AD  - Department of Biochemistry and Molecular Biology, University of Salamanca, 
      Salamanca 37007, Spain; IBSAL (Instituto de Investigacion Biomedica de 
      Salamanca), Salamanca 37007, Spain.
FAU - Llanillo, Marcial
AU  - Llanillo M
AD  - Department of Biochemistry and Molecular Biology, University of Salamanca, 
      Salamanca 37007, Spain; IBSAL (Instituto de Investigacion Biomedica de 
      Salamanca), Salamanca 37007, Spain.
FAU - Hernandez-Hernandez, Angel
AU  - Hernandez-Hernandez A
AD  - Department of Biochemistry and Molecular Biology, University of Salamanca, 
      Salamanca 37007, Spain; IBSAL (Instituto de Investigacion Biomedica de 
      Salamanca), Salamanca 37007, Spain. Electronic address: angelhh@usal.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150903
PL  - United States
TA  - Stem Cell Reports
JT  - Stem cell reports
JID - 101611300
RN  - 0 (RNA, Small Interfering)
RN  - 0 (beta Catenin)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatase, Non-Receptor Type 13)
RN  - EC 3.1.3.48 (Ptpn13 protein, mouse)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/metabolism
MH  - Cell Adhesion
MH  - Cell Communication
MH  - Cell Line
MH  - Cells, Cultured
MH  - Gene Expression Regulation, Developmental
MH  - HEK293 Cells
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Humans
MH  - *Lymphopoiesis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Protein Tyrosine Phosphatase, Non-Receptor Type 13/genetics/*metabolism
MH  - RNA Interference
MH  - RNA, Small Interfering/genetics
MH  - Stem Cell Niche
MH  - *Thrombopoiesis
MH  - beta Catenin/genetics/*metabolism
PMC - PMC4624939
EDAT- 2015/09/08 06:00
MHDA- 2016/08/03 06:00
PMCR- 2015/09/03
CRDT- 2015/09/08 06:00
PHST- 2015/02/23 00:00 [received]
PHST- 2015/08/04 00:00 [revised]
PHST- 2015/08/05 00:00 [accepted]
PHST- 2015/09/08 06:00 [entrez]
PHST- 2015/09/08 06:00 [pubmed]
PHST- 2016/08/03 06:00 [medline]
PHST- 2015/09/03 00:00 [pmc-release]
AID - S2213-6711(15)00237-4 [pii]
AID - 10.1016/j.stemcr.2015.08.003 [doi]
PST - ppublish
SO  - Stem Cell Reports. 2015 Oct 13;5(4):516-31. doi: 10.1016/j.stemcr.2015.08.003. 
      Epub 2015 Sep 3.