PMID- 26345838
OWN - NLM
STAT- MEDLINE
DCOM- 20160607
LR  - 20150908
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 3
DP  - 2015 Aug 7
TI  - Role of inflammatory responses in the pathogenesis of human cerebral aneurysm.
PG  - 9062-70
LID - 10.4238/2015.August.7.15 [doi]
AB  - We aimed to detect expressional profiles of intracellular cell adhesion 
      molecule-1 (ICAM-1), nuclear factor-kappa B (NF-kappaB), and monocyte chemoattractant 
      protein-1 (MCP-1) in human cerebral aneurysm, in order to investigate the effect 
      of chronic inflammation on the pathogenesis of cerebral aneurysm. Samples from 40 
      cases of human cerebral aneurysms diagnosed at our hospital were selected along 
      with 20 normal cerebral artery samples. Western blotting and immunohistochemical 
      (IHC) staining were used to reveal expressional profiles of ICAM-1 and NF-kappaB in 
      the aneurysmal wall of patients and normal cerebral artery tissues. Reverse 
      transcription (RT)-PCR was employed to detect changes in transcript levels of 
      MCP-1 mRNA. Western blotting showed significantly higher expressions of ICAM-1 
      and NF-kappaB in patients with cerebral aneurysm compared to the normal group (P < 
      0.01), which was consistent with IHC staining results. RT-PCR revealed 
      significantly higher MCP-1 transcripts in cerebral aneurysm tissues compared to 
      the normal group (P < 0.01), in addition to a positive relationship between 
      ICAM-1 and NF-kappaB expression levels. In conclusion, expression levels of ICAM-1, 
      NF-kappaB, and MCP-1 in patients are significantly elevated, suggesting an enhanced 
      chronic inflammatory response and a significant correlation between inflammatory 
      factors/adhesion molecules and the pathogenesis of cerebral aneurysm.
FAU - Fan, X J
AU  - Fan XJ
FAU - Zhao, H D
AU  - Zhao HD
FAU - Yu, G
AU  - Yu G
FAU - Zhong, X L
AU  - Zhong XL
FAU - Yao, H
AU  - Yao H
FAU - Yang, Q D
AU  - Yang QD
LA  - eng
PT  - Journal Article
DEP - 20150807
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Adult
MH  - Cerebral Arteries/pathology
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Female
MH  - Gene Expression Regulation
MH  - Humans
MH  - Inflammation/*genetics/pathology
MH  - Intercellular Adhesion Molecule-1/*biosynthesis/genetics
MH  - Intracranial Aneurysm/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - NF-kappa B/*biosynthesis/genetics
MH  - RNA, Messenger/biosynthesis
EDAT- 2015/09/09 06:00
MHDA- 2016/06/09 06:00
CRDT- 2015/09/09 06:00
PHST- 2015/09/09 06:00 [entrez]
PHST- 2015/09/09 06:00 [pubmed]
PHST- 2016/06/09 06:00 [medline]
AID - gmr5611 [pii]
AID - 10.4238/2015.August.7.15 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Aug 7;14(3):9062-70. doi: 10.4238/2015.August.7.15.