PMID- 26346217
OWN - NLM
STAT- MEDLINE
DCOM- 20160811
LR  - 20220331
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Sep 8
TI  - Bacterial inhibition potential of 3D rapid-prototyped magnesium-based porous 
      composite scaffolds--an in vitro efficacy study.
PG  - 13775
LID - 10.1038/srep13775 [doi]
LID - 13775
AB  - Bone infections are common in trauma-induced open fractures with bone defects. 
      Therefore, developing anti-infection scaffolds for repairing bone defects is 
      desirable. This study develoepd novel Mg-based porous composite scaffolds with a 
      basal matrix composed of poly(lactic-co-glycolicacid) (PLGA) and tricalcium 
      phosphate (TCP). A unique low-temperature rapid prototyping technology was used 
      to fabricate the scaffolds, including PLGA/TCP (PT), PLGA/TCP/5%Mg (PT5M), 
      PLGA/TCP/10%Mg (PT10M), and PLGA/TCP/15%Mg (PT15M). The bacterial adhesion and 
      biofilm formation of Staphylococcus aureus were evaluated. The results indicated 
      that the Mg-based scaffolds significantly inhibited bacterial adhesion and 
      biofilm formation compared to PT, and the PT10M and PT15M exhibited significantly 
      stronger anti-biofilm ability than PT5M. In vitro degratation tests revealed that 
      the degradation of the Mg-based scaffolds caused an increase of pH, Mg(2+) 
      concentration and osmolality, and the increased pH may be one of the major 
      contributing factors to the antibacterial function of the Mg-based scaffolds. 
      Additionally, the PT15M exhibited an inhibitory effect on cell adhesion and 
      proliferation of MC3T3-E1 cells. In conclusion, the PLGA/TCP/Mg scaffolds could 
      inhibit bacterial adhesion and biofilm formation, and the PT10M scaffold was 
      considered to be an effective composition with considerable antibacterial ability 
      and good cytocompatibility.
FAU - Ma, Rui
AU  - Ma R
AD  - Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, 
      Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, China.
AD  - Department of Orthopedic Surgery, the Second Affiliated Hospital of Xi'an 
      Jiaotong University, Xi'an, Shanxi Province, China.
FAU - Lai, Yu-xiao
AU  - Lai YX
AD  - Translational Medicine Research and Development Centre, Institute of Biomedical 
      and Health Engineering, Shenzhen Institute of Advanced Technology, The Chinese 
      Academy of Sciences, Shenzhen, China.
FAU - Li, Long
AU  - Li L
AD  - Translational Medicine Research and Development Centre, Institute of Biomedical 
      and Health Engineering, Shenzhen Institute of Advanced Technology, The Chinese 
      Academy of Sciences, Shenzhen, China.
FAU - Tan, Hong-lue
AU  - Tan HL
AD  - Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, 
      Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, China.
FAU - Wang, Jia-li
AU  - Wang JL
AD  - Department of Orthopedics and Traumatology, The Chinese University of Hong Kong, 
      Hong Kong, China.
FAU - Li, Ye
AU  - Li Y
AD  - Translational Medicine Research and Development Centre, Institute of Biomedical 
      and Health Engineering, Shenzhen Institute of Advanced Technology, The Chinese 
      Academy of Sciences, Shenzhen, China.
FAU - Tang, Ting-ting
AU  - Tang TT
AD  - Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, 
      Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of 
      Medicine, Shanghai, China.
FAU - Qin, Ling
AU  - Qin L
AD  - Translational Medicine Research and Development Centre, Institute of Biomedical 
      and Health Engineering, Shenzhen Institute of Advanced Technology, The Chinese 
      Academy of Sciences, Shenzhen, China.
AD  - Department of Orthopedics and Traumatology, The Chinese University of Hong Kong, 
      Hong Kong, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150908
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Phosphates)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - I38ZP9992A (Magnesium)
RN  - K4C08XP666 (tricalcium phosphate)
SB  - IM
MH  - Animals
MH  - *Anti-Bacterial Agents
MH  - Bacterial Adhesion
MH  - Biofilms
MH  - *Bone Substitutes/chemistry
MH  - Calcium Phosphates/chemistry
MH  - Cell Adhesion
MH  - Cell Line
MH  - Cell Proliferation
MH  - Lactic Acid/chemistry
MH  - *Magnesium/chemistry
MH  - Mice
MH  - Microbial Sensitivity Tests
MH  - Microbial Viability
MH  - Polyglycolic Acid/chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Staphylococcus aureus/drug effects/ultrastructure
MH  - *Tissue Scaffolds/chemistry
PMC - PMC4561899
EDAT- 2015/09/09 06:00
MHDA- 2016/08/12 06:00
PMCR- 2015/09/08
CRDT- 2015/09/09 06:00
PHST- 2015/03/18 00:00 [received]
PHST- 2015/08/05 00:00 [accepted]
PHST- 2015/09/09 06:00 [entrez]
PHST- 2015/09/09 06:00 [pubmed]
PHST- 2016/08/12 06:00 [medline]
PHST- 2015/09/08 00:00 [pmc-release]
AID - srep13775 [pii]
AID - 10.1038/srep13775 [doi]
PST - epublish
SO  - Sci Rep. 2015 Sep 8;5:13775. doi: 10.1038/srep13775.