PMID- 26346906
OWN - NLM
STAT- MEDLINE
DCOM- 20160217
LR  - 20151027
IS  - 1365-3083 (Electronic)
IS  - 0300-9475 (Linking)
VI  - 82
IP  - 6
DP  - 2015 Dec
TI  - Enhanced Humoral Responses Induced by Targeting of Antigen to Murine Dendritic 
      Cells.
PG  - 515-22
LID - 10.1111/sji.12387 [doi]
AB  - Dendritic cells (DCs) are superior in their ability to induce and control 
      adaptive immune responses. These qualities have motivated the hypothesis that 
      targeted delivery of antigen to DCs in vivo may be an effective way of enhancing 
      immunization. Recent results show that antigen targeted to certain DC surface 
      molecules may indeed induce robust immune responses. Targeting of antigen to DCs 
      can be accomplished by the means of monoclonal antibodies. This study compared 
      the humoral responses induced in mice by in vivo targeting of DCs using 
      monoclonal antibodies specific for CD11c, CD36, CD205, Clec6A, Clec7A, Clec9A, 
      Siglec-H and PDC-TREM. The results demonstrate that antigen delivery to different 
      targets on DCs in vivo gives rise to humoral responses that differ in strength. 
      Targeting of antigen to CD11c, CD36, CD205, Clec6A, Clec7A and PDC-TREM induced 
      significantly stronger antibody responses compared to non-targeted 
      isotype-matched controls. Targeting of Clec9A and Siglec-H did not lead to 
      efficient antibody responses, which may be due to unfavourable properties of the 
      targeting antibody, in which case, other antibodies with the same specificity 
      might elicit a different outcome. Anti-CD11c was additionally used for 
      elucidating the impact of the route of vaccination, and the results showed only 
      minor differences between the antibody responses induced after immunization 
      either s.c., i.v. or i.p. Altogether, these data show that targeting of different 
      surface molecules on DCs result in very different antibody responses and that, 
      even in the absence of adjuvants, strong humoral responses was induced.
CI  - (c) 2015 The Foundation for the Scandinavian Journal of Immunology.
FAU - Pugholm, L H
AU  - Pugholm LH
AD  - Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
AD  - Laboratory of Immunology, Department of Health Science and Technology, Aalborg 
      University, Aalborg, Denmark.
FAU - Petersen, L R
AU  - Petersen LR
AD  - Laboratory of Immunology, Department of Health Science and Technology, Aalborg 
      University, Aalborg, Denmark.
FAU - Sondergaard, E K L
AU  - Sondergaard EK
AD  - Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
FAU - Varming, K
AU  - Varming K
AD  - Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark.
FAU - Agger, R
AU  - Agger R
AD  - Laboratory of Immunology, Department of Health Science and Technology, Aalborg 
      University, Aalborg, Denmark.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens)
RN  - 0 (Antigens, Surface)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Antibody Formation/immunology
MH  - Antigens/*administration & dosage
MH  - Antigens, Surface/*immunology
MH  - Dendritic Cells/*immunology
MH  - Drug Delivery Systems
MH  - Female
MH  - Immunity, Humoral/immunology
MH  - Immunization/*methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Rats
EDAT- 2015/09/09 06:00
MHDA- 2016/02/18 06:00
CRDT- 2015/09/09 06:00
PHST- 2015/06/09 00:00 [received]
PHST- 2015/08/25 00:00 [accepted]
PHST- 2015/09/09 06:00 [entrez]
PHST- 2015/09/09 06:00 [pubmed]
PHST- 2016/02/18 06:00 [medline]
AID - 10.1111/sji.12387 [doi]
PST - ppublish
SO  - Scand J Immunol. 2015 Dec;82(6):515-22. doi: 10.1111/sji.12387.