PMID- 26348370
OWN - NLM
STAT- MEDLINE
DCOM- 20160803
LR  - 20181113
IS  - 1897-5631 (Electronic)
IS  - 0239-8508 (Print)
IS  - 0239-8508 (Linking)
VI  - 53
IP  - 3
DP  - 2015
TI  - Histological changes of testes in growth hormone transgenic mice with high plasma 
      level of GH and insulin-like growth factor-1.
PG  - 249-58
LID - 10.5603/fhc.a2015.0024 [doi]
AB  - INTRODUCTION: Overexpression of growth hormone (GH) leads to increase in 
      insulin-like growth factor-1 (IGF-1) plasma level, stimulation of growth and 
      increase in body size, organomegaly and reduced body fat. The action of GH 
      affects all the organs and transgenic mice that overexpress bovine GH (bGH mice) 
      serve as convenient model to study somatotropic axis. Male mice overexpressing GH 
      are fertile, however, they show reduced overall lifespan as well as reproductive 
      life span. The aim of the study was to evaluate the morphology and expression of 
      androgen receptor (AR) and luteinizing hormone receptor (LHR) of bGH mice testes. 
      MATERIAL AND METHODS: The experiment was performed on 6 and 12 month-old bGH male 
      mice and 6 and 12 month-old wild type (WT) littermates (8 animals in each group). 
      The morphology of testes was evaluated on deparaffinized sections stained by the 
      periodic acid-Schiff (PAS) method. Expression of AR and LHR was investigated by 
      immunohistochemistry and diameters of seminiferous tubules (ST) were measured on 
      round cross sections of ST. RESULTS: We noted larger testes in 6-month bGH mice 
      as compared to normal WT littermates. The morpho-logical observations revealed 
      essentially normal structure of Leydig cells, seminiferous epithelium and other 
      morphological structures. However, some changes like tubules containing only 
      Sertoli cells, tubules with arrested spermatogenesis or vacuoles in seminiferous 
      epithelium could be attributed to the overexpression of GH. In contrast to WT 
      mice, 12 month-old bGH mice displayed first symptoms of testicular aging. The 
      immunoexpres-sion of AR and LHR was decreased in 12 month-old bGH males as 
      compared to 12 month-old WT mice and younger animals. CONCLUSION: Chronic 
      exposure to elevated GH level accelerates testicular aging and thus potentially 
      may change response of Leydig cells to LH and Sertoli and germ cells to 
      testosterone.
FAU - Piotrowska, Katarzyna
AU  - Piotrowska K
AD  - Department of Physiology, Pomeranian Medical University, Szczecin, Poland. 
      piot.kata@gmail.com.
FAU - Sluczanowska-Glabowska, Sylwia
AU  - Sluczanowska-Glabowska S
FAU - Kucia, Magda
AU  - Kucia M
FAU - Bartke, Andrzej
AU  - Bartke A
FAU - Laszczynska, Maria
AU  - Laszczynska M
FAU - Ratajczak, Mariusz Z
AU  - Ratajczak MZ
LA  - eng
GR  - R01 AG019899/AG/NIA NIH HHS/United States
PT  - Journal Article
DEP - 20150908
PL  - Poland
TA  - Folia Histochem Cytobiol
JT  - Folia histochemica et cytobiologica
JID - 8502651
RN  - 0 (Receptors, Androgen)
RN  - 0 (Receptors, LH)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Gene Expression Regulation/drug effects
MH  - Growth Hormone/*blood/genetics
MH  - *Insulin-Like Growth Factor I
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Receptors, Androgen/genetics
MH  - Receptors, LH/genetics
MH  - Testis/*drug effects/*ultrastructure
PMC - PMC4820830
MID - NIHMS772670
OTO - NOTNLM
OT  - GH transgenic mice
OT  - IHC
OT  - LHR
OT  - androgen receptor
OT  - testis histology
EDAT- 2015/09/09 06:00
MHDA- 2016/08/04 06:00
PMCR- 2016/04/05
CRDT- 2015/09/09 06:00
PHST- 2015/01/22 00:00 [received]
PHST- 2015/09/07 00:00 [accepted]
PHST- 2015/09/08 00:00 [revised]
PHST- 2015/09/09 06:00 [entrez]
PHST- 2015/09/09 06:00 [pubmed]
PHST- 2016/08/04 06:00 [medline]
PHST- 2016/04/05 00:00 [pmc-release]
AID - VM/OJS/J/40921 [pii]
AID - 10.5603/fhc.a2015.0024 [doi]
PST - ppublish
SO  - Folia Histochem Cytobiol. 2015;53(3):249-58. doi: 10.5603/fhc.a2015.0024. Epub 
      2015 Sep 8.